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    Methods to generate gastrointestinal epithelial tissue constructs
    82.
    发明授权
    Methods to generate gastrointestinal epithelial tissue constructs 有权

    公开(公告)号：US11193110B2

    公开(公告)日：2021-12-07

    申请号：US15545456

    申请日：2016-01-29

    Applicant: The University of North Carolina at Chapel Hill

    Inventor： Nancy Allbritton ,  Yuli Wang ,  Christopher Sims ,  Scott Magness ,  Scott Bultman

    IPC: C12N5/071 ,  C12Q1/08

    Abstract: A method of making a live cell construct is carried out by: (a) providing a non-cellular support having a top surface and a bottom surface, (b) contacting live undifferentiated cells to the non-cellular support, and then (c) propagating a gastrointestinal epithelial cell monolayer on said top surface. In some embodiments, the live cells in the monolayer include: (i) undifferentiated cells (e.g., stem or progenitor cells); and (ii) optionally, but in some embodiments preferably, differentiated cells (e.g., enterocytes, Paneth cells, enteroendocrine cells, tuft cells, microcells, intra-epithelial lymphocytes, and/or goblet cells). Constructs formed by such methods and methods of using the same (e.g., in high through-put screening) are also described.

    Methods and apparatus for spectroscopic identification and/or calibrated quantification of surface concentration of materials
    84.
    发明授权
    Methods and apparatus for spectroscopic identification and/or calibrated quantification of surface concentration of materials 有权

    公开(公告)号：US11022490B1

    公开(公告)日：2021-06-01

    申请号：US16904471

    申请日：2020-06-17

    Applicant: Photon Systems, Inc.

    Inventor： Michael R. Reid

    IPC: G01J3/02 ,  C12Q1/24 ,  G01J3/28 ,  C12Q1/08

    Abstract: Apparatus and methods for creating deposits of uniformly spaced or uniformly overlapping droplets of selected chemicals where each droplet has an a priori known amount of the selected chemical or chemicals is taught (including biological and microbial materials). In some embodiments the deposits may be used as samples of different but known concentrations that may be used to calibrate spectroscopic inspection instruments to enable such instruments to not only provide identification in situ of unknown materials but also to provide calibrated and traceable surface concentrations of such materials. In some embodiments, such calibrated instruments may be used in enhanced processes for validating the cleanliness of manufacturing surfaces such as surfaces of equipment used in the preparation of pharmaceuticals, food, or semiconductor devices. Such instruments may be used to ensure adequate purity, or non-contamination, of surfaces of products themselves or packaging materials or of locations where such products will be used. Such calibrated instruments may also be useful in detecting cleanliness of non-manufacturing surfaces where contamination may be of concern, whether they be public or private spaces such as laboratories, restaurants, airports, satellites or other spacecraft. In some embodiments, such instruments may range from deep UV instruments to far infrared instruments or beyond.

    Test device
    85.
    发明授权
    Test device 有权

    公开(公告)号：US10793821B2

    公开(公告)日：2020-10-06

    申请号：US15544302

    申请日：2016-01-22

    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

    Inventor： Chihiro Uematsu ,  Muneo Maeshima

    IPC: C12M1/34 ,  C12M1/32 ,  C12Q1/08 ,  G02B21/26 ,  G06T7/00 ,  G06T1/00 ,  C12Q1/04 ,  G02B21/36

    Abstract: The invention provides a technology for promptly determining bacterial identification or an antimicrobial susceptibility testing. In the invention, first, a state where the bacteria are divided is monitored by performing microscopic observation with respect to the shape or the number of bacteria in each of wells of a culture plate for bacterial identification culture or the antimicrobial susceptibility testing. In addition, the shape, the number or the area of the bacteria are interpreted from the image obtained by the microscopic observation whether or not the bacteria proliferate at a stage from an induction phase to a logarithmic phase, and the time-dependent changes thereof are made into a graph. From the graph, it is determined whether or not the bacteria proliferate for each measurement, the determination results are displayed on the screen, and accordingly, the result of the antimicrobial susceptibility is provided every time when the measurement is performed (FIG. 12).

    Microorganism enumeration methods
    88.
    发明授权
    Microorganism enumeration methods 有权
    微生物计数方法

    公开(公告)号：US09476084B2

    公开(公告)日：2016-10-25

    申请号：US14684049

    申请日：2015-04-10

    Applicant: MAK Wood, Inc.

    Inventor： Mark A. Brudnak ,  Eric J. Baer

    IPC: C12Q1/06 ,  C12Q1/08

    CPC classification number: C12Q1/06 ,  C12Q1/08

    Abstract: This disclosure provides methods for enumeration of microorganisms within a freeze-dried powder suspected of containing Lactobacilli or Bifidobacteria.

    Abstract translation: 本公开提供了在怀疑含有乳杆菌或双歧杆菌的冷冻干燥粉末中计数微生物的方法。

    Micro-organism test apparatus and methods of using the same
    89.
    发明授权
    Micro-organism test apparatus and methods of using the same 有权
    微生物试验装置及其使用方法

    公开(公告)号：US09260740B2

    公开(公告)日：2016-02-16

    申请号：US11922785

    申请日：2005-06-23

    Applicant: Rosemary Katherine Cameron Sharpin

    Inventor： Rosemary Katherine Cameron Sharpin

    IPC: C12Q1/08 ,  C12Q1/04 ,  C12M1/00 ,  C12M1/34

    CPC classification number: C12Q1/08 ,  C12M23/34 ,  C12M41/36 ,  C12Q1/04 ,  Y10T436/173076

    Abstract: The contamination of foodstuffs, additives, cosmetics, pharmaceuticals and the like by undesirable micro-organisms is threat to public health. On occasion, the level of bacteria that conventional testing techniques are designed to look for is so low that the test result for the bacterium is “negative”. However, the bacterium although present in un-detectably small amounts may multiply under the right conditions and given the shelf life of the product, may be able to recover to an extent whereby public health is threatened. This problem is alleviated by the provision of micro-organism testing apparatus comprising (a) an incubator, and (b) a multi-compartment resealable container provided with or adapted to receive a growth medium in one compartment and having a growth medium additive in another compartment, the compartments being separated by a barrier whose removal or puncture will expose the medium to the additive.

    Abstract translation: 不良微生物对食品，添加剂，化妆品，药​​品等的污染对公众健康构成威胁。 有时，传统测试技术设计的细菌水平很低，使得细菌的检测结果为“阴性”。 然而，细菌虽然以不可检测的少量存在，但可以在合适的条件下繁殖并给予产品的保存期限，可以恢复到公共卫生受到威胁的程度。 通过提供微生物测试装置来减轻这个问题，微生物测试装置包括（a）培养箱和（b）多室可重新密封的容器，其提供或适于在一个隔室中接收生长培养基并且在另一个隔室中具有生长培养基添加剂 隔间，隔室被隔离物隔开，其移除或穿刺将介质暴露于添加剂。

    Method for preparing protein, DNA, and RNA from cell
    90.
    发明授权
    Method for preparing protein, DNA, and RNA from cell 有权
    从细胞中制备蛋白质，DNA和RNA的方法

    公开(公告)号：US09169480B2

    公开(公告)日：2015-10-27

    申请号：US13318337

    申请日：2010-05-26

    Applicant: Nobuko Yamamoto

    Inventor： Nobuko Yamamoto

    IPC: C12Q1/00 ,  C12Q1/08 ,  C12N5/00 ,  C12N9/00 ,  C12N15/10 ,  C07K1/34

    CPC classification number: C12N15/1017 ,  C07K1/34

    Abstract: An object of the present invention is to prepare DNA, RNA, and a protein from one cell and to provide a convenient preparation method with high reproducibility. To prepare a protein, DNA, and RNA from a cell, nuclear and cytoplasmic separation is performed, and a protein, DNA, and RNA are then extracted.

    Abstract translation: 本发明的目的是从一个细胞制备DNA，RNA和蛋白质，并提供方便的制备方法，具有高的再现性。 为了从细胞中制备蛋白质，DNA和RNA，进行核和细胞质分离，然后提取蛋白质，DNA和RNA。

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