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公开(公告)号：US11326185B2
公开(公告)日：2022-05-10
申请号：US16176525
申请日：2018-10-31
申请人： LONZA BIOLOGICS PLC , PFIZER INC.
发明人： James Rance , Robert Young , Michael J. Agostino , Mark Moffat , Lin Zhang , Baohong Zhang
摘要： The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
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公开(公告)号：US09783771B2
公开(公告)日：2017-10-10
申请号：US14885827
申请日：2015-10-16
申请人： Lonza Biologics PLC
发明人： Mohsan Khan
CPC分类号： C12M23/58 , C12M21/08 , C12M27/02 , C12M27/08 , C12M27/20 , C12M29/06 , C12N5/00 , C12N2527/00
摘要： The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
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公开(公告)号：US09670446B2
公开(公告)日：2017-06-06
申请号：US14885802
申请日：2015-10-16
申请人： Lonza Biologics PLC
发明人： Mohsan Khan
CPC分类号： C12M23/58 , C12M21/08 , C12M27/02 , C12M27/08 , C12M27/20 , C12M29/06 , C12N5/00 , C12N2527/00
摘要： The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
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公开(公告)号：US20150167020A1
公开(公告)日：2015-06-18
申请号：US14409283
申请日：2013-06-20
申请人： LONZA BIOLOGICS PLC , PFIZER INC.
发明人： James Rance , Robert Young , Michael J. Agostino , Mark Moffat , Lin Zhang , Baohong Zhang
CPC分类号： C12N15/907 , C07K16/00 , C07K2317/14 , C07K2317/24 , C12N15/10 , C12N15/90 , C12N2800/22 , C12N2800/30 , C12P21/00
摘要： The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e.g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
摘要翻译： 本发明涉及稳定和高产出的位点特异性整合（SSI）宿主细胞，例如， 中国仓鼠卵巢（CHO）衍生的宿主细胞，生产和使用它们的方法。
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公开(公告)号：US08906231B2
公开(公告)日：2014-12-09
申请号：US13394560
申请日：2010-09-10
申请人： Mark R. Whickman , Sam Mansoor
发明人： Mark R. Whickman , Sam Mansoor
摘要： The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
摘要翻译： 本发明提供了用于纯化多肽的方法和自动化系统，包括在纯化后直接过滤含有多肽的溶液。
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公开(公告)号：US20130295596A1
公开(公告)日：2013-11-07
申请号：US13881170
申请日：2011-10-27
申请人： Dietmar Lang , Elaine B. Martin , Gary A. Montague , Christopher J. O'Malley , Tracy S. Root , Carol M. Trim , Jane F. Povey , Christopher M. Smales , Andrew J. Racher
发明人： Dietmar Lang , Elaine B. Martin , Gary A. Montague , Christopher J. O'Malley , Tracy S. Root , Carol M. Trim , Jane F. Povey , Christopher M. Smales , Andrew J. Racher
IPC分类号： G01N27/62
CPC分类号： G01N33/6848 , G01N27/62 , G01N33/5005 , H01J49/0031 , H01J49/164 , H01J49/40
摘要： The present invention relates to a process for the prediction of cell culture performance data of sample cells, a process for the isolation of said cells and a device for the prediction of cell culture performance data of sample cells.
摘要翻译： 本发明涉及预测样本细胞的细胞培养性能数据的方法，分离所述细胞的方法和用于预测样品细胞的细胞培养性能数据的装置。
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公开(公告)号：US08538706B2
公开(公告)日：2013-09-17
申请号：US13270931
申请日：2011-10-11
申请人： Johan Desmet , Geert Meersseman , Nathalie Boutonnet , Jurgen Pletinckx , Krista De Clercq , Ignace Lasters
发明人： Johan Desmet , Geert Meersseman , Nathalie Boutonnet , Jurgen Pletinckx , Krista De Clercq , Ignace Lasters
摘要： The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.
摘要翻译： 本发明涉及肽/蛋白复合物的基于定量结构的亲和评分方法。 更具体地说，本发明包括一种基于应用于肽/受体复合物的全原子结构表示的高度特异性的力场函数（例如CHARMM）来操作的方法。 在详细的旋转异构体取样之后进行受控能量细化，对总亲和力的肽侧链贡献进行评分。 本发明的方法还包括从填充有显性水分子的溶剂盒中的各个氨基酸的模拟估算脱水能量并应用用于评估肽/受体复合物相互作用的相同的力场函数的从头方法。
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公开(公告)号：US20130211808A2
公开(公告)日：2013-08-15
申请号：US13472080
申请日：2012-05-15
CPC分类号： G06F19/16
摘要： The present invention provides methods and apparatus for analyzing a protein structure.
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公开(公告)号：US20080312425A1
公开(公告)日：2008-12-18
申请号：US10595671
申请日：2005-08-30
IPC分类号： C07K1/00
CPC分类号： B01D15/1871 , B01D15/362 , B01D15/363 , B01D15/3809 , C07K16/065 , G01N30/461
摘要： A novel method for purifying antibody and other product protein concomitant with removing aggregates made up from single product protein species is devised.
摘要翻译： 设计了一种用于纯化抗体和其他产品蛋白质的新方法，同时除去由单一产品蛋白质种类组成的聚集体。
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公开(公告)号：US20240141020A1
公开(公告)日：2024-05-02
申请号：US18264831
申请日：2021-03-11
发明人： Julia ARMSTRONG , James GRAHAM
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , C07K2317/14 , C07K2317/41
摘要： The present application is directed to methods for modulating afucosylation of an antibody product that is produced in a bioreactor. The methods include the addition of mannose to the bioreactor to control the afucosylation of the antibody product, including increasing by afucosylation by 1% or more, relative to untreated bioreactor products.
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