公开(公告)号：US20240302339A1

公开(公告)日：2024-09-12

申请号：US18600390

申请日：2024-03-08

申请人： PROMEGA CORPORATION

发明人： Ian MAROZAS ,  Zhiyang ZENG ,  Wenhui ZHOU ,  Mike VALLEY ,  Jolanta VIDUGIRIENE ,  James CALI ,  Tony LAUER

IPC分类号： G01N31/22 ,  C12M1/34 ,  G01N21/64

CPC分类号： G01N31/225 ,  C12M41/46 ,  G01N21/6408 ,  G01N2201/06193

摘要： Provided herein are assays utilizing oxygen-sensitive fluorescent materials for the detection of oxygen. In particular, oxygen-sensitive fluorescent particles are provided for monitoring the oxygen consumption rate and metabolic fitness of living cells.

公开(公告)号：US20240252654A1

公开(公告)日：2024-08-01

申请号：US18252083

申请日：2021-10-19

申请人： University of Dundee ,  Promega Corporation

发明人： Alessio Ciulli ,  Andrea Testa ,  Chiara Maniaci ,  Nikolai Makukhin ,  Satomi Imaide ,  Danette Daniels ,  Kristin Riching

IPC分类号： A61K47/55 ,  A61K47/60

CPC分类号： A61K47/55 ,  A61K47/60

摘要： This invention particularly relates to trifunctional PROTACs of formula I as described herein which bind to a protein within the Bromo- and Extra-Terminal (BET) family of proteins, and especially to PROTACs including small molecule E3 ubiquitin ligase protein binding ligand compounds which induce preferential degradation of the BRD2 protein within the bromodomain of the BET family of proteins.

公开(公告)号：US20240182434A1

公开(公告)日：2024-06-06

申请号：US18440576

申请日：2024-02-13

申请人： Promega Corporation

发明人： Wenhui Zhou ,  Kimberly K. Knoche ,  Douglas R. Storts ,  Min Zhou ,  Poncho Meisenheimer

IPC分类号： C07D307/42 ,  C07C317/28 ,  C07D307/80 ,  C07D311/58 ,  C12N9/12 ,  C12P19/34

CPC分类号： C07D307/42 ,  C07C317/28 ,  C07D307/80 ,  C07D311/58 ,  C12N9/1252 ,  C12P19/34 ,  C12Y207/07007

摘要： The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

公开(公告)号：US20240132859A1

公开(公告)日：2024-04-25

申请号：US18312441

申请日：2023-05-04

申请人： Promega Corporation

发明人： Michael P. Killoran ,  Lance P. Encell ,  Thomas Kirkland ,  Thomas Machleidt ,  Rachel Friedman Ohana ,  Robin Hurst ,  Mark A. Klein ,  Karilyn Porter ,  Rahele Esmatpour ,  Debayan De Bakshi

IPC分类号： C12N9/14

CPC分类号： C12N9/14 ,  C12Y308/01

摘要： Provided herein modified dehalogenases have extended surface loop regions that provide a location for internal fusion insertions and modulate binding interaction and activation of environmentally-sensitive chemistries.

公开(公告)号：US20240068009A1

公开(公告)日：2024-02-29

申请号：US18457868

申请日：2023-08-29

申请人： Promega Corporation

发明人： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC分类号： C12Q1/66 ,  A61K49/00 ,  C07D487/04 ,  C07D519/00 ,  G01N33/58 ,  H04N1/60

CPC分类号： C12Q1/66 ,  A61K49/0013 ,  C07D487/04 ,  C07D519/00 ,  G01N33/581 ,  H04N1/6041 ,  G01N2333/90241

摘要： Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

公开(公告)号：US20240059697A1

公开(公告)日：2024-02-22

申请号：US18320633

申请日：2023-05-19

申请人： PROMEGA CORPORATION

发明人： Joel R. Walker ,  Thomas A. Kirkland ,  Wenhui Zhou ,  Mary P. Hall ,  Harry Tetsuo Uyeda

IPC分类号： C07D487/04 ,  A61K49/00 ,  G01N21/76

CPC分类号： C07D487/04 ,  G01N21/763 ,  A61K49/0021

摘要： Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

公开(公告)号：US20230382875A1

公开(公告)日：2023-11-30

申请号：US18137896

申请日：2023-04-21

申请人： Promega Corporation

发明人： Zhiyang Zeng ,  Wenhui Zhou ,  Douglas R. Storts ,  Poncho Meisenheimer ,  Ian Marozas ,  Andrew Taft ,  Spencer Hermanson ,  Connor Fitzgerald ,  Kasen Riemersma

IPC分类号： C07D265/30 ,  C12N15/10 ,  C07D223/14 ,  C07D249/16 ,  C12Q1/686

CPC分类号： C07D265/30 ,  C12N15/1013 ,  C07D223/14 ,  C07D249/16 ,  C12Q1/686

摘要： Provided herein are compounds, compositions, and methods for rapid isolation of nucleic acids from a sample.

公开(公告)号：US20230366045A1

公开(公告)日：2023-11-16

申请号：US18176918

申请日：2023-03-01

申请人： Promega Corporation

发明人： Subhanjan Mondal ,  Nathan Feirer ,  Min Zhou ,  Michael Scurria ,  Wenhui Zhou ,  Said Goueli ,  James J. Cali

IPC分类号： C12Q1/70 ,  C12Q1/6851 ,  C07D221/12 ,  C07F15/00 ,  C07D235/20 ,  C07F9/6558 ,  C07D417/06 ,  C07D417/14 ,  C07D495/04

CPC分类号： C12Q1/701 ,  C12Q1/6851 ,  C07D221/12 ,  C07F15/0093 ,  C07D235/20 ,  C07F9/65583 ,  C07D417/06 ,  C07D417/14 ,  C07D495/04 ,  C12Q1/6806

摘要： Provided herein multifunctional compounds that bind and modify nucleic acids, forming crosslinks between the nucleic acid strands. In particular embodiments, the multifunctional nucleic-acid binding/modifying compounds are selective for nucleic acids of non-viable cells and viruses (i.e., they are excluded from or degraded within viable cells and viruses), and therefore find use in methods for detecting or discriminating viable and non-viable cells/viruses and nucleic acids therefrom.

公开(公告)号：US20230357266A1

公开(公告)日：2023-11-09

申请号：US18312418

申请日：2023-05-04

申请人： Promega Corporation

发明人： Matthew A. Larsen ,  Rachel Friedman Ohana ,  Wenhui Zhou ,  Ce Shi ,  Jian Cao ,  Robin Hurst ,  Mark A. Klein ,  Hui Wang ,  Weiwei An ,  Karilyn Porter ,  Thomas Machleidt

IPC分类号： C07D495/04 ,  B01J23/46 ,  B01J35/00 ,  C07B59/00

CPC分类号： C07D495/04 ,  B01J23/468 ,  B01J23/462 ,  B01J35/004 ,  C07B59/002 ,  C07B2200/05

摘要： Provided herein are compounds, compositions, systems, and methods for photoactivatable labeling, which can be actuated within biological systems. In particular, compounds disclosed herein include vinyl-extended-aryl azide moieties that undergo photoactivation to generate reactive intermediates, which can form covalent linkages with biomolecules. The photoactivation can be conducted by a variety of mechanisms including ultraviolet (UV) irradiation, visible light irradiation, or energy transfer (e.g., from a photocatalyst). The compounds also include functional moieties that provide useful functionalities, for example detection of biomolecules, such as fluorophores, capture elements (e.g., biotin), or reactive moieties (e.g., click handles) and bifunctional moieties comprising a bioactive compound and a detection/capture element.

公开(公告)号：US20230341410A1

公开(公告)日：2023-10-26

申请号：US18150018

申请日：2023-01-04

申请人： Promega Corporation

发明人： Melanie Dart ,  Lance P. Encell ,  Thomas Kirkland ,  Thomas Machleidt ,  Matthew Robers ,  Brock F. Binkowski ,  Keith Wood ,  Ce Shi

IPC分类号： G01N33/68 ,  G01N33/569 ,  G01N21/64 ,  G01N33/58

CPC分类号： G01N33/6845 ,  G01N33/56916 ,  C12Y113/12007 ,  G01N21/6428 ,  G01N33/581 ,  G01N33/582 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2333/90241 ,  G01N2500/04 ,  G01N2500/10

摘要： Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).