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    METHOD FOR SECRETORY PRODUCTION OF GLYCOPROTEIN HAVING HUMAN-TYPE SUGAR CHAIN USING PLANT CELL
    2.
    发明申请
    METHOD FOR SECRETORY PRODUCTION OF GLYCOPROTEIN HAVING HUMAN-TYPE SUGAR CHAIN USING PLANT CELL 有权
    使用植物细胞分泌生产人类糖链的糖蛋白的方法

    公开(公告)号：US20140377799A1

    公开(公告)日：2014-12-25

    申请号：US14252215

    申请日：2014-04-14

    申请人： Phyton Holdings, LLC

    发明人： Kazuhito Fujiyama ,  Tatsuji Seki ,  Toshiomi Yoshida

    IPC分类号： C12P21/00 ,  C12N9/08

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/00 ,  C12N9/0065 ,  C12N9/1051 ,  C12N15/8257

    摘要： A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.

    摘要翻译： 一种用于分泌具有人型糖链的糖蛋白的方法，包括将能够进行半乳糖残基的转移反应的酶的基因导入非还原性末端乙酰葡糖胺残基的步骤，以及基因 异源糖蛋白，以获得转化的植物细胞，培养植物细胞的步骤和回收植物细胞的培养基的步骤。

    ENHANCED PRODUCTION OF TAXOL AND TAXANES BY CELL CULTURES OF TAXUS SPECIES
    4.
    发明申请
    ENHANCED PRODUCTION OF TAXOL AND TAXANES BY CELL CULTURES OF TAXUS SPECIES 失效
    通过细菌培养物种增强TAXOL和TAXANES的生产

    公开(公告)号：US20110086397A1

    公开(公告)日：2011-04-14

    申请号：US12972064

    申请日：2010-12-17

    申请人： Venkataraman BRINGI ,  Prakash G. Kadkade ,  Christopher L. Prince ,  Braden L. Roach

    发明人： Venkataraman BRINGI ,  Prakash G. Kadkade ,  Christopher L. Prince ,  Braden L. Roach

    IPC分类号： C12P17/02

    CPC分类号： C12N5/0025 ,  C12P15/00 ,  C12P17/02

    摘要： This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T. chinensis is particularly enhanced by use of one or more of these conditions, yield of taxanes for all Taxus species has been found to benefit from use of these conditions.

    摘要翻译： 本发明提供了可以从所有已知的紫杉属物种（例如短枝红，加拿大，西葫芦，芽孢杆菌，球形花粉，花蓟马，堇菜，培养基）中以非常高的产量生产紫杉醇，浆果赤霉素III和其他紫杉醇样化合物或紫杉烷类的方法 和中华。 已经发现了培养条件（即培养基组成和操作模式）的特殊修饰，以提高所有紫杉属种类细胞培养物中各种紫杉烷的产量。 特别优选的增强剂包括银离子或络合物，茉莉酸（特别是甲酯），生长素相关的生长调节剂和苯丙素途径的抑制剂，如3,4-亚甲二氧基-6-硝基肉桂酸。 这些增强剂可以单独使用或彼此组合使用或其它收率增强条件使用。 通过使用这些条件中的一种或多种，​​特别地增强了来自中华根瘤菌植物细胞培养物的紫杉烷的产率，已经发现所有紫杉属物种的紫杉烷的产率受益于使用这些条件。

    PRODUCTION OF INGENOL, INGENOL ESTERS AND/OR TIGLIAN-3-ONE DERIVATIVES BY EUPHORBIACEAE PLANT CELL SUSPENSION CULTURES
    9.
    发明申请
    PRODUCTION OF INGENOL, INGENOL ESTERS AND/OR TIGLIAN-3-ONE DERIVATIVES BY EUPHORBIACEAE PLANT CELL SUSPENSION CULTURES 审中-公开

    公开(公告)号：US20180042194A1

    公开(公告)日：2018-02-15

    申请号：US15559565

    申请日：2016-03-18

    申请人： Phyton Holdings, LLC

    发明人： Gilbert Gorr ,  David Alexander Ullisch ,  Yantree Devi Sankar-Thomas ,  Thomas Selge ,  Thomas Leibold ,  Harald Heckenmüller

    IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  A61K36/47

    CPC分类号： A01H4/001 ,  A61K36/47 ,  C12N5/0025 ,  C12N5/04

    摘要： The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives. The present invention further relates to a plant cell biomass comprising plant cells obtained from the suspension cell culture of the invention, and comprising Ingenol and/or one or more Ingenol esters and/or one or more Tiglian-3-one derivatives. Also, the present invention relates to cryopreserved cells of the plant suspension cell culture of the invention as well as to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives based on these cryopreserved cells.