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公开(公告)号：US20080155705A1
公开(公告)日：2008-06-26
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
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2.发明申请Novel Plant Acyltransferases Specific For Long-Chained, Multiply Unsaturated Fatty Acids 有权新型植物酰基转移酶特异于长链多重不饱和脂肪酸公开(公告)号：US20110023185A1
公开(公告)日：2011-01-27
申请号：US12844938
申请日：2010-07-28
申请人： ANDREAS RENZ , JÖRG BAUER , MARGIT FRENTZEN , NURSEN SÖZER , STOBART KEITH , THOMAS FRASER , COLIN M. LAZARUS , BAOXIU QI , AMINE ABBADI , ERNST HEINZ
发明人： ANDREAS RENZ , JÖRG BAUER , MARGIT FRENTZEN , NURSEN SÖZER , STOBART KEITH , THOMAS FRASER , COLIN M. LAZARUS , BAOXIU QI , AMINE ABBADI , ERNST HEINZ
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含核酸序列的核酸序列和构建体，载体和生物体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。
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公开(公告)号：US20090209774A1
公开(公告)日：2009-08-20
申请号：US12417171
申请日：2009-04-02
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译： 本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。
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4.发明授权Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids 有权植物酰基转移酶特异于长链，多不饱和脂肪酸公开(公告)号：US08354569B2
公开(公告)日：2013-01-15
申请号：US12844938
申请日：2010-07-28
申请人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
发明人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含核酸序列的核酸序列和构建体，载体和生物体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。
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公开(公告)号：US07537920B2
公开(公告)日：2009-05-26
申请号：US10547447
申请日：2004-01-29
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译： 本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。
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6.发明申请Method for Producing Polyunsaturated Long-Chain Fatty Acids in Transgenic Organisms 有权生物多不饱和长链脂肪酸在转基因生物中的应用公开(公告)号：US20090094707A1
公开(公告)日：2009-04-09
申请号：US11793854
申请日：2005-12-20
申请人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
发明人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity am introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译： 本发明涉及在生物体中生产多不饱和脂肪酸的方法，根据该方法将编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽的核酸引入生物体中。 有利地，所述核酸序列可以任选与编码脂肪酸或脂质代谢多肽的其它核酸序列一起在转基因生物体中表达。 本发明还涉及本发明的核酸序列，含有本发明核酸序列的核酸构建体，含有本发明核酸序列和/或核酸构建体的载体，以及含有所述核酸序列，核酸构建体和/ 或向量。 本发明还涉及根据本发明方法生产的油，脂和/或脂肪酸及其用途。
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公开(公告)号：US08486671B2
公开(公告)日：2013-07-16
申请号：US12417171
申请日：2009-04-02
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
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8.发明授权Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids 有权植物酰基转移酶特异于长链，多不饱和脂肪酸公开(公告)号：US07855321B2
公开(公告)日：2010-12-21
申请号：US10552013
申请日：2004-03-26
申请人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
发明人： Andreas Renz , Jörg Bauer , Margit Frentzen , Nursen Sözer , Stobart Keith , Thomas Fraser , Colin M. Lazarus , Baoxiu Qi , Amine Abbadi , Ernst Heinz
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
摘要翻译： 本发明涉及通过向生物体中引入编码具有酰基转移酶活性的多肽的核酸来生产生物体中长链多不饱和脂肪酸的方法。 如果合适的话，这些核酸序列与编码脂肪酸或脂质代谢生物合成的多肽的其它核酸序列一起可以有利地在生物体中表达。 此外，本发明涉及一种具有升高的多链多不饱和脂肪酸含量的油和/或三酰基甘油酯的生产方法。 本发明还涉及包含本发明的核酸序列的核酸序列，核酸构建体，载体和生物，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物 ，核酸构建体和/或载体。 本发明的另一部分涉及通过根据本发明的方法生产的油，脂质和/或脂肪酸及其用途。
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公开(公告)号：US09433228B2
公开(公告)日：2016-09-06
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
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10.发明授权Method for producing polyunsaturated long-chain fatty acids in transgenic organisms 有权在转基因生物体内生产多不饱和长链脂肪酸的方法公开(公告)号：US07871804B2
公开(公告)日：2011-01-18
申请号：US11793854
申请日：2005-12-20
申请人： Petra Cirpus , Jörg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
发明人： Petra Cirpus , Jörg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译： 本发明涉及在生物体中生产多不饱和脂肪酸的方法，根据该方法将编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽的核酸引入生物体中。 有利地，核酸序列可以在转基因生物任选地与编码脂肪酸或脂质代谢多肽的其它核酸序列一起表达。 本发明还涉及本发明的核酸序列，含有本发明的核酸序列的核酸构建体，含有本发明核酸序列和/或核酸构建体的载体，以及含有核酸序列，核酸构建体和/ 或向量。 本发明还涉及根据本发明方法生产的油，脂和/或脂肪酸及其用途。
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