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公开(公告)号：US20240123452A1
公开(公告)日：2024-04-18
申请号：US18320362
申请日：2023-05-19
发明人： Kirk M. Ririe , Aaron D. Wernerehl , Christopher P. Pasko , Ali Laayoun , Carole Vachon , Agnès Dupont-Filliard , Laurent Mesta , Andrew C. Hatch , Erik W. Huynh , David E. Jones
CPC分类号： B01L7/525 , B01L3/502761 , B01L2200/0668 , B01L2200/0689 , B01L2300/0636 , B01L2300/0816 , B01L2300/1811 , B01L2300/1816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/043 , C12Q1/6844
摘要： Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.
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公开(公告)号：US11959132B2
公开(公告)日：2024-04-16
申请号：US17329844
申请日：2021-05-25
发明人： Randy P. Rasmussen , Robert John Crisp , Andrew Clinton Hemmert , Elizabeth Barker Campbell , Thomas Charles Robbins , David J. Eyre
IPC分类号： C12P19/34 , C12Q1/6851 , C12Q1/686 , C12Q1/689 , C12Q1/70
CPC分类号： C12Q1/686 , C12Q1/6851 , C12Q1/689 , C12Q1/705 , C12Q1/6851 , C12Q2527/107 , C12Q2537/165
摘要： Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.
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公开(公告)号：US11268141B2
公开(公告)日：2022-03-08
申请号：US16087724
申请日：2017-03-20
IPC分类号： C12Q1/686 , C12Q1/6851 , G16B30/00 , C07H21/04 , C12M1/26 , C12P19/34 , C12Q1/6806 , C12Q1/70 , C12Q1/689
摘要： Methods, sample vessels, and instruments are provided for quantitative and semi-quantitative amplification.
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公开(公告)号：US11041193B2
公开(公告)日：2021-06-22
申请号：US16037948
申请日：2018-07-17
发明人： Randy P. Rasmussen , Robert John Crisp , Andrew Clinton Hemmert , Elizabeth Barker Campbell , Thomas Charles Robbins , David J. Eyre
IPC分类号： C12P19/34 , C12Q1/686 , C12Q1/6851 , C12Q1/689 , C12Q1/70
摘要： Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.
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公开(公告)号：US20200318160A1
公开(公告)日：2020-10-08
申请号：US16906870
申请日：2020-06-19
发明人： Mark Aaron Poritz , Kirk Max Ririe , Christopher Paul Pasko , Ann Michelle Demogines , Robert John Crisp , Margarita Rogatcheva , Robert Cornelius Trauscht , Matthew Kam Jones , Tyler Lane Healy
IPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6874
摘要： Method and sample vessels are provided for amplification and sequencing of nucleic acids in a sample.
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公开(公告)号：US09102911B2
公开(公告)日：2015-08-11
申请号：US13751560
申请日：2013-01-28
CPC分类号： C12Q1/686 , B01L3/502 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/044 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2400/0481 , B01L2400/049 , B01L2400/0655 , C12M33/00
摘要： Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.
摘要翻译： 提供设备，容器和方法用于在封闭环境中进行生物分析。 说明性的生物学分析包括高密度核酸扩增和检测和免疫PCR。
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公开(公告)号：US08940526B2
公开(公告)日：2015-01-27
申请号：US13765249
申请日：2013-02-12
发明人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
CPC分类号： B01L7/52 , B01L3/502723 , B01L3/50273 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0481 , B01L2400/049 , B01L2400/0644 , B01L2400/065 , B01L2400/0655 , B01L2400/0677 , B01L2400/0683 , C12Q1/6844
摘要： Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyzes include nucleic acid amplification and detection and immuno-PCR.
摘要翻译： 提供设备，容器和方法用于在封闭环境中进行生物分析。 说明性的生物学分析包括核酸扩增和检测和免疫PCR。
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公开(公告)号：US20140283945A1
公开(公告)日：2014-09-25
申请号：US14356837
申请日：2012-11-09
CPC分类号： B01L3/502 , A61J1/1425 , A61J1/20 , A61M5/14228 , B01L3/5082 , B01L7/52 , B01L2200/026 , B01L2300/042 , B01L2300/043 , B01L2300/0838 , B01L2300/0851 , B01L2300/0858 , B01L2300/0864 , B01L2400/0406 , B01L2400/049
摘要： Vials for loading a fluid system are provided. The illustrative vials are configured to minimize air bubbles from being transferred into the fluid system. Certain vial embodiments are provided with filters to allow microbes to pass into the fluidic system, while retaining larger particulate matter.
摘要翻译： 提供了用于装载流体系统的小瓶。 说明性的小瓶被配置为使气泡最小化以将气泡转移到流体系统中。 某些小瓶实施例设置有过滤器，以允许微生物进入流体系统，同时保留较大的颗粒物质。
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公开(公告)号：US20220055033A1
公开(公告)日：2022-02-24
申请号：US17372082
申请日：2021-07-09
IPC分类号： B01L3/00 , B01L7/00 , C12N1/06 , C12Q1/6806
摘要： Instruments and methods for amplifying nucleic acids in a sample provided in a flexible, self-contained, substantially closed sample container.
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公开(公告)号：US20210180112A1
公开(公告)日：2021-06-17
申请号：US17048771
申请日：2019-04-18
发明人： Kate Mariel Broadbent , Robert John Crisp , Ann Michelle Demogines , Matthew Kam Jones , Margarita Rogatcheva , Usha K. Spaulding
IPC分类号： C12Q1/6806 , C12Q1/689
摘要： Methods and kits for normalization and quantification of sequencing data.
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