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    Rapid epidemiologic typing of bacteria
    6.
    发明授权
    Rapid epidemiologic typing of bacteria 有权

    公开(公告)号：US10421991B2

    公开(公告)日：2019-09-24

    申请号：US14938242

    申请日：2015-11-11

    申请人： BioFire Diagnostics, LLC ,  University of Utah Research Foundation

    发明人： Anne Jeannette Blaschke-Bonkowsky ,  Mark Aaron Poritz

    IPC分类号： C12P19/34 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/689

    摘要： Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.

    Organism identification panel
    10.
    发明授权
    Organism identification panel 有权

    公开(公告)号：US10724081B2

    公开(公告)日：2020-07-28

    申请号：US15660609

    申请日：2017-07-26

    申请人： BioFire Diagnostics, LLC ,  University of Utah Research Foundation

    发明人： Mark Aaron Poritz ,  Anne Jeannette Blaschke-Bonkowsky

    IPC分类号： C12P19/34 ,  C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/689

    摘要： Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance.