公开(公告)号：US20100248315A1

公开(公告)日：2010-09-30

申请号：US11882456

申请日：2007-08-01

申请人： Marie-Gabrielle Beuzelin-Ollivier ,  Bastian Chevreux ,  Manuela Dalluegge ,  Marina Van Gelder ,  Markus G. Goese ,  Corina Hauk ,  Bertus P. Koekman ,  Connie Lee ,  Anne F. Mayer ,  Anja Meury ,  Nigel J. Mouncey ,  Dick Schipper ,  Masako Shinjoh ,  Christine Toepfer ,  Adrianus W.H. Vollebregt

发明人： Marie-Gabrielle Beuzelin-Ollivier ,  Bastian Chevreux ,  Manuela Dalluegge ,  Marina Van Gelder ,  Markus G. Goese ,  Corina Hauk ,  Bertus P. Koekman ,  Connie Lee ,  Anne F. Mayer ,  Anja Meury ,  Nigel J. Mouncey ,  Dick Schipper ,  Masako Shinjoh ,  Christine Toepfer ,  Adrianus W.H. Vollebregt

IPC分类号： C12P17/04 ,  C12N1/21

CPC分类号： C12P17/04 ,  C12N9/0008

摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及能够直接生产L-抗坏血酸（以下也称为维生素C）的新鉴定的微生物。 本发明还涉及包含参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽 以及它们的功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：US5108918A

公开(公告)日：1992-04-28

申请号：US305532

申请日：1989-02-01

申请人： Martien A. M. Groenen ,  Annemarie E. Veenstra ,  Pieter van Solingen ,  Bertus P. Koekman

发明人： Martien A. M. Groenen ,  Annemarie E. Veenstra ,  Pieter van Solingen ,  Bertus P. Koekman

IPC分类号： C07K14/375 ,  C12N9/10 ,  C12N9/88 ,  C12N15/10 ,  C12N15/52 ,  C12N15/80 ,  C12P37/00

CPC分类号： C12N15/52 ,  C07K14/375 ,  C12N15/1034 ,  C12N15/80 ,  C12N9/1029 ,  C12N9/88 ,  C12P37/00

摘要： Novel methods and compositions are provided for the enhanced production of secondary metabolites. Methods are provided for identifying sequences which when transformed into secondary metabolite producing hosts, enhance the production of secondary metabolite. The process is exemplified for penicillin. In addition, the P. chrysogenum acyltransferase gene is isolated and sequenced.

摘要翻译： 提供了新的方法和组合物用于增强二级代谢产物的产生。 提供了用于鉴定当转化为次生代谢物产生宿主时的序列的方法，增强次级代谢产物的产生。 该过程示例为青霉素。 另外，产黄青素酰基转移酶基因被分离并测序。

公开(公告)号：US08318462B2

公开(公告)日：2012-11-27

申请号：US11882456

申请日：2007-08-01

申请人： Marie-Gabrielle Beuzelin-Ollivier ,  Bastian Chevreux ,  Manuela Dalluege ,  Marina Van Gelder ,  Markus G. Goese ,  Corina Hauk ,  Bertus P. Koekman ,  Connie Lee ,  Anne F. Mayer ,  Anja Meury ,  Nigel J. Mouncey ,  Dick Schipper ,  Masako Shinjoh ,  Christine Toepfer ,  Adrianus W. H. Vollebregt

发明人： Marie-Gabrielle Beuzelin-Ollivier ,  Bastian Chevreux ,  Manuela Dalluege ,  Marina Van Gelder ,  Markus G. Goese ,  Corina Hauk ,  Bertus P. Koekman ,  Connie Lee ,  Anne F. Mayer ,  Anja Meury ,  Nigel J. Mouncey ,  Dick Schipper ,  Masako Shinjoh ,  Christine Toepfer ,  Adrianus W. H. Vollebregt

IPC分类号： C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0008

摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及能够直接生产L-抗坏血酸（以下也称为维生素C）的新鉴定的微生物。 本发明还涉及包含参与维生素C合成的蛋白质的基因的多核苷酸序列。本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸和片段编码的新多肽 以及它们的功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：US5462862A

公开(公告)日：1995-10-31

申请号：US8688

申请日：1993-01-25

申请人： Martinus A. M. Groenen ,  Annemarie E. Veenstra ,  Pieter Van Solingen ,  Bertus P. Koekman ,  Lucia H. M. Van Der Voort ,  Juan F. Martin ,  Santiago Gutierrez ,  Bruno Diez ,  Emilio Alvarez ,  Jose L. Barredo ,  Christina Esmahan

发明人： Martinus A. M. Groenen ,  Annemarie E. Veenstra ,  Pieter Van Solingen ,  Bertus P. Koekman ,  Lucia H. M. Van Der Voort ,  Juan F. Martin ,  Santiago Gutierrez ,  Bruno Diez ,  Emilio Alvarez ,  Jose L. Barredo ,  Christina Esmahan

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/375 ,  C12N1/15 ,  C12N1/21 ,  C12N9/00 ,  C12N9/10 ,  C12N9/52 ,  C12N9/88 ,  C12N15/10 ,  C12N15/52 ,  C12N15/80 ,  C12P37/00 ,  C12Q1/68 ,  C12R1/04 ,  C12R1/82 ,  C12P21/06 ,  C07H17/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C07K14/375 ,  C12N15/1034 ,  C12N15/52 ,  C12N15/80 ,  C12N9/1029 ,  C12N9/88 ,  C12P37/00

摘要： Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.

摘要翻译： 聚簇抗生素生物合成基因用于改善微生物中抗生素的生产和用于分离参与抗生素生物合成的其它基因。 本发明通过改良产黄青霉菌的生产得到例证，其中分离另一个聚集的生物合成基因，并与产黄青霉菌中的青霉素生物合成基因的表达。