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    Methods for cell-based combinatorial logic
    1.
    发明授权
    Methods for cell-based combinatorial logic 失效
    基于单元的组合逻辑的方法

    公开(公告)号：US07020560B2

    公开(公告)日：2006-03-28

    申请号：US09949015

    申请日：2001-09-06

    Applicant: Gary S. Sayler ,  James T. Fleming ,  Bruce Applegate ,  Michael L. Simpson

    Inventor： Gary S. Sayler ,  James T. Fleming ,  Bruce Applegate ,  Michael L. Simpson

    IPC: G01N21/00 ,  G01N27/00 ,  G01N33/48

    CPC classification number: C12Q1/6897 ,  B82Y10/00 ,  G01N33/5005 ,  G01N33/5438 ,  G06N3/002

    Abstract: A method for cell-based combinatorial logic includes the steps of providing at least one genetically engineered cell, the genetically engineered cell having at least one transcriptional unit. The transcriptional unit includes a gene and a promoter, wherein application of a stimulus to the promoter results in the expression of a gene product. An energetic or chemical stimulus is applied to activate the promoter, wherein the detection of an output signal corresponds to the presence of a gene product. The cell can include a plurality of transcriptional units configured to form logic gates. The logic gates of a plurality of cells can be operably interconnected by release of output signals, such as chemical stimuli.

    Abstract translation: 一种用于基于小区的组合逻辑的方法包括以下步骤：提供至少一个经遗传工程化的细胞，所述基因工程细胞具有至少一个转录单位。 转录单位包括基因和启动子，其中向启动子施加刺激物导致基因产物的表达。 施加能量或化学刺激以激活启动子，其中输出信号的检测对应于基因产物的存在。 该小区可以包括被配置成形成逻辑门的多个转录单元。 多个单元的逻辑门可以通过释放诸如化学刺激的输出信号来可操作地互连。

    Bioluminescent biosensor device
    3.
    发明授权
    Bioluminescent biosensor device 失效
    生物发光生物传感器装置

    公开(公告)号：US06544729B2

    公开(公告)日：2003-04-08

    申请号：US09910360

    申请日：2001-07-20

    Applicant: Gary S. Sayler ,  Steven A. Ripp ,  Bruce Applegate

    Inventor： Gary S. Sayler ,  Steven A. Ripp ,  Bruce Applegate

    IPC: C12O100

    CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N15/63 ,  C12N2795/10343 ,  C12Q1/04 ,  C12Q1/6897 ,  G01N21/763 ,  G01N33/56911 ,  G01N35/00069

    Abstract: Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.

    Abstract translation: 公开了用于检测细菌的方法和装置，所述方法和装置基于对经过遗传修饰的噬菌体的一种或多种所选菌株的识别和感染以在噬菌体感染后在细菌中产生诱导物分子。 诱导物分子从受感染的细菌中释放出来，并被设计成在诱导物刺激时发射生物发光的遗传修饰的细菌生物转运体细胞被检测。 生物发光信号的自动扩增允许检测低浓度的细菌，而不需要样品富集。 还公开了基于所述技术的选择细菌的检测方法和用于检测选择性细菌的试剂盒。

    Continuous-flow solar ultraviolet disinfection system for drinking water
    5.
    发明授权
    Continuous-flow solar ultraviolet disinfection system for drinking water 有权
    饮用水连续太阳紫外线消毒系统

    公开(公告)号：US09546100B2

    公开(公告)日：2017-01-17

    申请号：US14342080

    申请日：2012-08-29

    Applicant: Ernest R Blatchley, III ,  Eric Gentil Mbonimpa ,  Bruce Applegate ,  Bryan Vadheim

    Inventor： Ernest R Blatchley, III ,  Eric Gentil Mbonimpa ,  Bruce Applegate ,  Bryan Vadheim

    IPC: G01N23/10 ,  C02F1/32 ,  G06F17/50 ,  C02F1/00

    CPC classification number: C02F1/32 ,  C02F1/008 ,  C02F1/325 ,  C02F2303/04 ,  G06F17/5009 ,  Y02W10/37

    Abstract: Potable drinking water is a scarce resource in many parts of developing countries, especially rural areas. Due to limited financial means of these countries, low cost point-of-use systems are thought to be appropriate technology to treat water. Systems using solar ultraviolet (UV) radiation could be successful since many vulnerable countries are located where solar radiation is intense and abundant throughout the year. The goal of this disclosure is to develop a simple and low cost point-of-use solar UV reactor to disinfect water. In this disclosure wavelength-dependent microbial dose-response behavior was investigated using surrogates to pathogenic microbes. A solar radiation prediction method based on the SMARTS model was used to predict solar UV intensity as function of geographic location and time. A numerical modeling procedure using the discrete ordinate (DO) model and CFD software (FLUENT) was used to simulate UV dose (distribution) delivery to microorganisms. Then, the dose distribution was combined with the dose response behavior using a segregated flow model to predict microbial inactivation by the reactor. A prototype was produced and tested to validate the numerical modeling procedure. The inactivation results from the prototype were in agreement with numerical inactivation prediction. The modeling procedure permits parameters such as reactor dimensions and material properties to be varied to meet a treatment goal.

    Abstract translation: 饮用水是发展中国家，特别是农村地区许多地方稀缺的资源。 由于这些国家的经济手段有限，低成本的使用点系统被认为是处理水的合适技术。 使用太阳能紫外线（UV）辐射的系统可能是成功的，因为许多脆弱的国家都位于全年太阳辐射强烈和丰富的地方。 本公开的目的是开发一种简单且成本低的太阳能UV反应器来消毒水。 在该公开内容中，使用代谢病原体微生物研究波长依赖性微生物剂量 - 反应行为。 使用基于SMARTS模型的太阳辐射预测方法来预测太阳紫外线强度作为地理位置和时间的函数。 使用离散纵坐标（DO）模型和CFD软件（FLUENT）的数值模拟程序来模拟微生物的紫外线剂量（分布）传递。 然后，使用分离流动模型将剂量分布与剂量反应行为结合，以预测反应器的微生物灭活。 生产和测试了原型，以验证数值模拟程序。 原型的灭活结果与数值灭活预测一致。 建模过程允许改变诸如反应器尺寸和材料性质的参数以满足治疗目标。

    CONTINUOUS-FLOW SOLAR ULTRAVIOLET DISINFECTION SYSTEM FOR DRINKING WATER
    6.
    发明申请
    CONTINUOUS-FLOW SOLAR ULTRAVIOLET DISINFECTION SYSTEM FOR DRINKING WATER 有权
    连续流动太阳能超声波消毒水消毒系统

    公开(公告)号：US20140225002A1

    公开(公告)日：2014-08-14

    申请号：US14342080

    申请日：2012-08-29

    Applicant: Ernest R Blatchley, III ,  Eric Gentil Mbonimpa ,  Bruce Applegate ,  Bryan Vadheim

    Inventor： Ernest R Blatchley, III ,  Eric Gentil Mbonimpa ,  Bruce Applegate ,  Bryan Vadheim

    IPC: C02F1/32 ,  G06F17/50

    CPC classification number: C02F1/32 ,  C02F1/008 ,  C02F1/325 ,  C02F2303/04 ,  G06F17/5009 ,  Y02W10/37

    Abstract: Potable drinking water is a scarce resource in many parts of developing countries, especially rural areas. Due to limited financial means of these countries, low cost point-of-use systems are thought to be appropriate technology to treat water. Systems using solar ultraviolet (UV) radiation could be successful since many vulnerable countries are located where solar radiation is intense and abundant throughout the year. The goal of this disclosure is to develop a simple and low cost point-of-use solar UV reactor to disinfect water. In this disclosure wavelength-dependent microbial dose-response behavior was investigated using surrogates to pathogenic microbes. A solar radiation prediction method based on the SMARTS model was used to predict solar UV intensity as function of geographic location and time. A numerical modeling procedure using the discrete ordinate (DO) model and CFD software (FLUENT) was used to simulate UV dose (distribution) delivery to microorganisms. Then, the dose distribution was combined with the dose response behavior using a segregated flow model to predict microbial inactivation by the reactor. A prototype was produced and tested to validate the numerical modeling procedure. The inactivation results from the prototype were in agreement with numerical inactivation prediction. The modeling procedure permits parameters such as reactor dimensions and material properties to be varied to meet a treatment goal.

    Abstract translation: 饮用水是发展中国家，特别是农村地区许多地方稀缺的资源。 由于这些国家的经济手段有限，低成本的使用点系统被认为是处理水的合适技术。 使用太阳能紫外线（UV）辐射的系统可能是成功的，因为许多脆弱的国家都位于全年太阳辐射强烈和丰富的地方。 本公开的目的是开发一种简单且成本低的太阳能UV反应器来消毒水。 在该公开内容中，使用代谢病原体微生物研究波长依赖性微生物剂量 - 反应行为。 使用基于SMARTS模型的太阳辐射预测方法来预测太阳紫外线强度作为地理位置和时间的函数。 使用离散纵坐标（DO）模型和CFD软件（FLUENT）的数值模拟程序来模拟微生物的紫外线剂量（分布）传递。 然后，使用分离流动模型将剂量分布与剂量反应行为结合，以预测反应器的微生物灭活。 生产和测试了原型，以验证数值模拟程序。 原型的灭活结果与数值灭活预测一致。 建模过程允许改变诸如反应器尺寸和材料性质的参数以满足治疗目标。

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