利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

50 条记录 (耗时 0.036 秒)

    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof
    2.
    发明授权
    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof 有权
    S-腺苷-L-高半胱氨酸水解酶的可逆抑制剂及其用途

    公开(公告)号：US08168643B2

    公开(公告)日：2012-05-01

    申请号：US11649996

    申请日：2007-01-05

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： A61P37/06 ,  C07D473/34 ,  C07D487/04 ,  A61K31/52 ,  A61P31/14 ,  A61P35/00

    CPC分类号： C07D473/34 ,  A61K31/52 ,  A61K45/06 ,  G06F19/16 ,  G06F19/18 ,  A61K2300/00

    摘要： The present invention provides compositions and methods for reversibly inhibiting S-adenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.

    摘要翻译： 本发明提供了可逆抑制S-腺苷-L-高半胱氨酸（SAH）水解酶的组合物和方法。 本发明的化合物可以与抗出血性病毒感染剂，免疫抑制剂，同型半胱氨酸降低剂或抗肿瘤剂组合使用。 本发明的组合物和方法可用于预防和治疗出血性病毒感染，自身免疫疾病，自体移植排斥反应，肿瘤，高半胱氨酸尿症，心血管疾病，中风，阿尔茨海默病或糖尿病。

    METHODS FOR ASSAYING PERCENTAGE OF GLYCATED HEMOGLOBIN
    3.
    发明申请
    METHODS FOR ASSAYING PERCENTAGE OF GLYCATED HEMOGLOBIN 有权
    测定糖化血红蛋白百分比的方法

    公开(公告)号：US20110250627A1

    公开(公告)日：2011-10-13

    申请号：US13083357

    申请日：2011-04-08

    申请人： Chong-Sheng YUAN ,  Abhijit DATTA ,  Limin Liu

    发明人： Chong-Sheng YUAN ,  Abhijit DATTA ,  Limin Liu

    IPC分类号： C12Q1/28 ,  C12Q1/26

    CPC分类号： C12Q1/28 ,  G01N33/723

    摘要： The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase. The amount of hydrogen peroxide generated in the reaction is measured for determination of percentage of glycated hemoglobin in blood samples. The invention provides kits for performing the methods of the invention.

    摘要翻译： 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶催化的酶反应。 测定反应中产生的过氧化氢的量，以测定血液样品中糖化血红蛋白的百分比。 本发明提供了用于实施本发明方法的试剂盒。

    Methods for assaying percentage of glycated hemoglobin
    4.
    发明授权
    Methods for assaying percentage of glycated hemoglobin 有权
    测定糖化血红蛋白百分比的方法

    公开(公告)号：US07855079B2

    公开(公告)日：2010-12-21

    申请号：US11881179

    申请日：2007-07-25

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Limin Liu

    IPC分类号： G01N33/72 ,  G01N33/00

    CPC分类号： G01N33/723 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/25375

    摘要： The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. The invention provides kits for performing the methods of the invention.

    摘要翻译： 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶和过氧化物酶催化的酶反应 。 本发明提供了用于实施本发明方法的试剂盒。

    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof
    6.
    发明申请
    Reversible inhibitors of S-adenosyl-L-homocysteine hydrolase and uses thereof 审中-公开
    S-腺苷-L-高半胱氨酸水解酶的可逆抑制剂及其用途

    公开(公告)号：US20080171049A1

    公开(公告)日：2008-07-17

    申请号：US11894364

    申请日：2007-08-20

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： A61K39/395 ,  C07D473/00 ,  A61P35/00 ,  A61P37/00 ,  A61K31/52 ,  A61K39/38

    CPC分类号： C07D473/34 ,  A61K31/52 ,  A61K45/06 ,  G16B15/00 ,  G16B20/00 ,  A61K2300/00

    摘要： The present invention provides compositions and methods for reversibly inhibiting S-adenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.

    摘要翻译： 本发明提供了可逆抑制S-腺苷-L-高半胱氨酸（SAH）水解酶的组合物和方法。 本发明的化合物可以与抗出血性病毒感染剂，免疫抑制剂，同型半胱氨酸降低剂或抗肿瘤剂组合使用。 本发明的组合物和方法可用于预防和治疗出血性病毒感染，自身免疫疾病，自体移植排斥反应，肿瘤，高半胱氨酸尿症，心血管疾病，中风，阿尔茨海默病或糖尿病。

    METHODS AND COMPOSITIONS FOR ASSAYING HOMOCYSTEINE
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR ASSAYING HOMOCYSTEINE 有权
    用于测定半胱氨酸的方法和组合物

    公开(公告)号：US20060246529A1

    公开(公告)日：2006-11-02

    申请号：US10043787

    申请日：2002-01-10

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： C12Q1/34

    CPC分类号： G01N33/6815 ,  C12N9/14 ,  C12Q1/34 ,  C12Y303/01001

    摘要： The present invention relates to compositions and methods for assaying homocysteine (Hcy) and thus related moieties, e.g., S-adenosylhomocysteine (SAH) or adenosine. More particularly, assay methods that employ, mutant SAH hydrolase having binding affinity for Hcy, SAH or adenosine but has attenuated catalytic activity, are provided. The modified enzymes and fusion proteins containing the modified enzymes are also provided.

    摘要翻译： 本发明涉及用于测定同型半胱氨酸（Hcy）的组合物和方法，因此涉及相关部分，例如S-腺苷高半胱氨酸（SAH）或腺苷。 更具体地，提供了采用对Hcy，SAH或腺苷具有结合亲和力但具有减毒催化活性的突变体SAH水解酶的测定方法。 还提供了含有修饰酶的修饰的酶和融合蛋白。

    Methods and compositions for assaying homocysteine
    8.
    发明授权
    Methods and compositions for assaying homocysteine 有权
    用于测定同型半胱氨酸的方法和组合物

    公开(公告)号：US07097968B2

    公开(公告)日：2006-08-29

    申请号：US10801623

    申请日：2004-03-15

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    IPC分类号： C12Q1/00

    CPC分类号： C12Q1/34 ,  C12Q1/48 ,  C12Q1/485 ,  G01N33/6815

    摘要： This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. A kit for assaying homocysteine based on the same principle is also provided.

    摘要翻译： 本发明一般涉及同型半胱氨酸检测领域。 特别地，本发明提供了一种测定样品中同型半胱氨酸存在或浓度的方法，该方法包括：在Hcy转化反应中使包含或怀疑含有Hcy的样品与Hcy共底物和Hcy转化酶接触以形成Hcy 转化产物和Hcy共底物转化产物; 并评估Hcy共底物转化产物以确定样品中Hcy的存在，不存在和/或量。 还提供了用于基于相同原理测定同型半胱氨酸的试剂盒。

    Methods and compositions for assaying homocysteine
    9.
    发明申请
    Methods and compositions for assaying homocysteine 审中-公开

    公开(公告)号：US20060172362A1

    公开(公告)日：2006-08-03

    申请号：US11394457

    申请日：2006-03-31

    申请人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    发明人： Chong-Sheng Yuan ,  Abhijit Datta ,  Chao Dou

    IPC分类号： C12Q1/48 ,  C12Q1/37

    CPC分类号： C12Q1/34 ,  C12Q1/48 ,  C12Q1/485 ,  G01N33/6815

    摘要： This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. A kit for assaying homocysteine based on the same principle is also provided.

    Enzyme cycling based assays for alpha-methylacyl-CoA racemase
    10.
    发明申请
    Enzyme cycling based assays for alpha-methylacyl-CoA racemase 失效
    α-甲酰基-CoA消旋酶的酶循环测定

    公开(公告)号：US20060084133A1

    公开(公告)日：2006-04-20

    申请号：US11004477

    申请日：2004-12-03

    申请人： Chong-Sheng Yuan

    发明人： Chong-Sheng Yuan

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/533 ,  G01N33/574

    摘要： The present invention provides a method for assaying alpha-methylacyl-CoA racemase activity. In the assay, a sample containing an alpha-methylacyl-CoA racemase or suspected of containing an alpha-methylacyl-CoA racemase is contacted with (2R)-2-methylacyl-CoA. If alpha-methylacyl-CoA racemase is present in the sample, (2R)-2-methylacyl-CoA is converted into (2S)-2-methylacyl-CoA. The method then utilizes a cycling reaction system between (2S)-2-methylacyl-CoA and trans-2,3-dehydroacyl-CoA to generate a detectable signal that corresponds to the alpha-methylacyl-CoA racemase activity. Kits for assaying alpha-methylacyl-CoA racemase based on the same principle are also provided.

    摘要翻译： 本发明提供了测定α-甲酰基-CoA消旋酶活性的方法。 在该测定中，含有α-甲基酰基-CoA消旋酶或怀疑含有α-甲酰基-CoA消旋酶的样品与（2R）-2-甲酰基-CoA接触。 如果样品中存在α-甲基酰基-CoA消旋酶，则将（2R）-2-甲酰基-CoA转化为（2S）-2-甲酰基-CoA。 然后该方法利用（2S）-2-甲基酰基-CoA和反式-2,3-脱氢酰基-CoA之间的循环反应体系产生对应于α-甲酰基-CoA消旋酶活性的可检测信号。 还提供了基于相同原理测定α-甲基酰基-CoA消旋酶的试剂盒。