摘要:
The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.
摘要:
The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.
摘要:
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.
摘要:
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.
摘要:
The present invention is a method of producing porous beads, which comprises the steps of providing a first liquid phase comprising a bead matrix material and essentially edgy templating particle(s), said particle(s) being treated with a surface modifying agent; providing a second liquid phase which is immiscible with the first liquid phase; contacting the first phase and the second phase under conditions resulting in an emulsion of droplets comprised of the first liquid phase dispersed in the continuous second liquid phase; transforming the droplets to mesoporous beads by solidification of the liquid; and removing the templating particle(s) from the beads without causing any essential change of the surrounding bead, whereby hierarchical networks of pores are provided in the beads.
摘要:
The present invention is a process of manufacture of one or more polysaccharide beads, comprising generating an aerosol of an aqueous polysaccharide solution, cooling the droplets of said aerosol in air to initiate gelling thereof and collecting droplets as gelled beads in a liquid or on a surface, characterised by adding a hydrophilic vapour pressure-lowering agent to said polysaccharide solution.
摘要:
The present invention is a process of manufacture of one or more polysaccharide beads, comprising generating an aerosol of an aqueous polysaccharide solution, cooling the droplets of said aerosol in air to initiate gelling thereof and collecting droplets as gelled beads in a liquid or on a surface, characterised by adding a hydrophilic vapour pressure-lowering agent to said polysaccharide solution.
摘要:
The present invention is a method of producing porous beads, which comprises the steps of providing a first liquid phase comprising a bead matrix material and essentially edgy templating particle(s), said particle(s) being treated with a surface modifying agent; providing a second liquid phase which is immiscible with the first liquid phase; contacting the first phase and the second phase under conditions resulting in an emulsion of droplets comprised of the first liquid phase dispersed in the continuous second liquid phase; transforming the droplets to mesoporous beads by solidification of the liquid; and removing the templating particle(s) from the beads without causing any essential change of the surrounding bead, whereby hierarchical networks of pores are provided in the beads.
摘要:
The present invention relates to a microcarrier onto the surface of which a cationic compound has been immobilised via a guanidine group. The microcarrier is capable of attachment of cells, e.g. via charge-based interaction, and is advantageously used as a support in the culture of cells. Said compound may comprise one or two amino acids, such as arginine (Arg) or a dipeptide. The invention also relates to a method of preparing a polycationic microcarrier, which method comprises to immobilise a compound that comprises at least one guanidine group to an epoxide-activated substrate.
摘要:
In order to lease threads (F), in particular warp threads into guide elements (2) of a handling device (1), such as combs or reeds, the threads (F) are first leased in sequence into a leasing comb (11) and are fixed thereto. The threads (F) are subsequently guided through the device (1) by means of the leasing comb (11). During this process, the threads (F) are taken up by the guide elements (2).