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    Novel Carbonyl Reductase, Gene Therefor and Use Thereof
    3.
    发明申请
    Novel Carbonyl Reductase, Gene Therefor and Use Thereof 失效
    新型羰基还原酶，其基因及其用途

    公开(公告)号：US20090029430A1

    公开(公告)日：2009-01-29

    申请号：US11665065

    申请日：2005-10-20

    申请人： Noriyuki Kizaki ,  Mokoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    发明人： Noriyuki Kizaki ,  Mokoto Ueda ,  Daisuke Moriyama ,  Naoaki Taoka ,  Yoshihiko Yasohara

    IPC分类号： C12P7/02 ,  C07H21/04 ,  C07K14/00 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

    摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

    摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离的3-氧代-3-苯基丙腈的活性的多肽，以产生（R）-3-羟基-3-苯基丙腈，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。

    NADH OXIDASE MUTANT HAVING IMPROVED STABILITY AND USE THEREOF
    4.
    发明申请
    NADH OXIDASE MUTANT HAVING IMPROVED STABILITY AND USE THEREOF 有权
    具有改善稳定性的NADH氧化物突变体及其用途

    公开(公告)号：US20130030164A1

    公开(公告)日：2013-01-31

    申请号：US13574458

    申请日：2011-01-19

    申请人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

    发明人： Shinichi Yoshida ,  Akira Iwasaki ,  Motohisa Washida ,  Tozo Nishiyama ,  Daisuke Moriyama ,  Naoaki Taoka

    IPC分类号： C12N9/02 ,  C12N15/63 ,  C07H21/02 ,  C12N1/19 ,  C12N5/10 ,  C12P19/36 ,  C12N15/53 ,  C12N1/21

    CPC分类号： C12N9/0036 ,  C12N9/0065 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04 ,  C12P19/36 ,  C12Y106/03 ,  C12Y106/03001 ,  C12Y111/01001

    摘要： Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.

    摘要翻译： 来自变形链球菌的成水NADH氧化酶在工业生产实际使用的稳定性方面应进一步提高。 本发明的目的是提供通过蛋白质工程技术，通过改性水合NADH氧化酶获得的酶，其可用作由氧化还原酶催化的用于立体选择性氧化的NAD +再生系统，使得酶能够承受 长期使用而不显示其NAD +再生活性的活性降低，即稳定性提高的酶，以及提供有效生产光学活性醇或氨基酸等有用物质的方法。 本发明涉及通过适当地引入突变来提高来自变形链球菌的水形成NADH氧化酶的稳定性的酶修饰方法。

    Novel (S,S) -Butanediol Dehydrogenase, Gene for the Same, and Use of the Same
    6.
    发明申请
    Novel (S,S) -Butanediol Dehydrogenase, Gene for the Same, and Use of the Same 审中-公开
    新型（S，S） - 丁二醇脱氢酶，其基因及其用途

    公开(公告)号：US20110143406A1

    公开(公告)日：2011-06-16

    申请号：US12223319

    申请日：2007-02-02

    申请人： Daisuke Moriyama ,  Naoaki Taoka

    发明人： Daisuke Moriyama ,  Naoaki Taoka

    IPC分类号： C12P7/26 ,  C07H21/04 ,  C12N9/04 ,  C12N15/63 ,  C12N1/21 ,  C12P7/18

    CPC分类号： C12Y101/01304 ,  C12N9/0006 ,  C12P7/18 ,  C12P41/002

    摘要： The present invention has its object to provide a novel (S,S)-butanediol dehydrogenase. The present invention also has its object to provide a gene coding for the enzyme protein, a vector containing the gene, a transformant harboring the vector, and a method of producing an optically active alcohol using the transformant. The polypeptide according to one embodiment of the present invention has the physicochemical properties including actions such as: acting on (2S,3S)-2,3-butanediol to form (S)-acetoin in the presence of NAD+ as a coenzyme; and reducing 2,3-butanedione to form (2S,3S)-2,3-butanediol in the presence of NADH as a coenzyme.

    摘要翻译： 本发明的目的是提供一种新型（S，S） - 丁二醇脱氢酶。 本发明的目的还在于提供编码酶蛋白的基因，含有该基因的载体，携带该载体的转化体，以及使用该转化体制造光学活性醇的方法。 根据本发明一个实施方案的多肽具有包括以下作用的物理化学性质：如在NAD +作为辅酶存在下作用于（2S，3S）-2,3-丁二醇以形成（S） - 乙酰胸苷; 并在NADH作为辅酶存在下还原2,3-丁二酮以形成（2S，3S）-2,3-丁二醇。

    Process for Producing Optically Active Secondary Alcohol
    7.
    发明申请
    Process for Producing Optically Active Secondary Alcohol 审中-公开
    生产光活性二级醇的方法

    公开(公告)号：US20080038803A1

    公开(公告)日：2008-02-14

    申请号：US11885056

    申请日：2006-02-24

    申请人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

    发明人： Akira Iwasaki ,  Motohisa Washida ,  Naoaki Taoka ,  Daisuke Moriyama ,  Junzou Hasegawa

    IPC分类号： C12P7/02 ,  C12N1/00

    CPC分类号： C12P41/002 ,  C12N9/0008 ,  C12N9/0036 ,  C12P7/02 ,  C12P7/18

    摘要： The present invention provides a method enabling convenient production of an optically active secondary alcohol useful as a pharmaceutical intermediate, particularly an optically active 1,2-diol and an optically active 2-alkanol, from an enantiomer mixture thereof. An oxidizing enzyme source having the capability of selectively oxidizing one enantiomer of secondary alcohol is allowed to act on an enantiomer mixture of secondary alcohol in the presence of a reducing enzyme source having the capability of reverse enantio-selectively reducing a ketone derivative, to convert the enantiomer mixture into a substantially single enantiomer at a theoretical percent recovery of 100%, whereby an optically active secondary alcohol is produced.

    摘要翻译： 本发明提供了一种能够方便地制备可用作药物中间体，特别是光学活性的1,2-二醇和光学活性2-链烷醇的光学活性仲醇的方法。 在具有反向对映选择性还原酮衍生物的能力的还原酶源的存在下，使具有选择性氧化仲醇的对映异构体的氧化酶源作用于仲醇的对映体混合物， 对映异构体混合物变成基本上单一的对映体，理论回收率为100％，由此产生光学活性的仲醇。

    Process for producing optically active 3-hydroxypropionic ester derivative
    8.
    发明申请
    Process for producing optically active 3-hydroxypropionic ester derivative 有权
    光学活性3-羟基丙酸酯衍生物的制备方法

    公开(公告)号：US20060166342A1

    公开(公告)日：2006-07-27

    申请号：US10536768

    申请日：2003-12-08

    申请人： Naoaki Taoka ,  Daisuke Moriyama ,  Kohei Mori ,  Takahiro Oishi

    发明人： Naoaki Taoka ,  Daisuke Moriyama ,  Kohei Mori ,  Takahiro Oishi

    IPC分类号： C12P7/62

    CPC分类号： C12P7/62 ,  C07C67/313 ,  C07D317/60 ,  C12P17/04 ,  C12P41/002 ,  C07C69/716

    摘要： The present invention is to provide a process for simply producing an optically active 3-hydroxypropionic ester derivative useful as a medicament intermediate from an inexpensive material. More specifically, the present invention is directed to a process for producing an optically active 3-hydroxypropionic ester derivative comprising reacting an acetic ester derivative available at low cost with abase and a formic ester, thereby converting the acetic ester derivative into a 2-formylacetic ester derivative, and thereafter, stereospecifically reducing the formyl group of the derivative by use of an enzymatic source capable of stereoselectively reducing the formyl group of the derivative.

    摘要翻译： 本发明提供一种简单地制造用作廉价材料的药物中间体的光学活性3-羟基丙酸酯衍生物的方法。 更具体地说，本发明涉及一种光学活性3-羟基丙酸酯衍生物的制备方法，其包括将低成本获得的乙酸酯衍生物与甲酸酯反应，由此将乙酸酯衍生物转化为2-甲酰基乙酸酯 衍生物，然后通过使用能够立体选择性还原衍生物的甲酰基的酶源立体选择性还原衍生物的甲酰基。

    NOVEL CARBONYL REDUCTASE, GENE THEREFOR AND USE THEREOF
    10.
    发明申请
    NOVEL CARBONYL REDUCTASE, GENE THEREFOR AND USE THEREOF 审中-公开
    新型碳水化合物，其基因及其用途

    公开(公告)号：US20130149769A1

    公开(公告)日：2013-06-13

    申请号：US13093610

    申请日：2011-04-25

    申请人： Noriyuki KIZAKI ,  Makoto UEDA ,  Daisuke MORIYAMA ,  Naoaki TAOKA ,  Yoshihiko YASOHARA

    发明人： Noriyuki KIZAKI ,  Makoto UEDA ,  Daisuke MORIYAMA ,  Naoaki TAOKA ,  Yoshihiko YASOHARA

    IPC分类号： C12N9/00

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

    摘要： The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to product (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.

    摘要翻译： 本发明提供一种有效制备包含（R）-3-羟基-3-苯基丙腈的光学活性醇的方法。 本发明的特征之一是具有不对称还原从属于念珠菌属的微生物分离到3-（3-羟基-3-苯基丙腈）产物的3-氧代-3-苯基丙腈的活性的多肽，编码 多肽和产生多肽的转化体。 本发明的另一个特征是通过使用多肽或转化体还原羰基化合物如3-氧代-3-苯基丙腈来制备光学活性醇如（R）-3-羟基-3-苯基丙腈的方法 。