公开(公告)号：US20080038824A1

公开(公告)日：2008-02-14

申请号：US10587776

申请日：2005-01-27

申请人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

发明人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

IPC分类号： C12N15/00 ,  C07K14/00 ,  C07K16/00 ,  C07K16/28

CPC分类号： C12N15/87 ,  C07K16/2842

摘要： It is intended to provide a method whereby transfer efficiency can be improved under any conditions in the case of transferring (in particular, transfecting) a target substance that cannot easily be transferred into cells (for example, a DNA, a polypeptide, a sugar, a complex thereof or the like). Namely, a composition for elevating the transfer efficiency of a target substance into cells which contains a cell adhesion-related factor, and a device and a method using the composition.

摘要翻译： 旨在提供一种方法，其可以在转移（特别是转染）不容易转移到细胞中的目标物质（例如DNA，多肽，糖， 其复合物等）。 即，将目标物质的转移效率提高为含有细胞粘附相关因子的细胞的组合物，以及使用该组合物的装置和方法。

公开(公告)号：US20100144038A1

公开(公告)日：2010-06-10

申请号：US10594349

申请日：2004-03-03

申请人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

发明人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

IPC分类号： C12N15/09 ,  C07K14/00 ,  C12N5/06 ,  C07K14/78

CPC分类号： C12N15/87 ,  C07K14/78 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/31

摘要： The present invention provides a method capable of improving the efficiency of introducing a target substance (e.g., DNA, polypeptides, sugars, or complexes thereof), which is difficult to introduce (particularly, transfect) into a cell in any circumstances. Particularly, the present invention provides a composition for increasing the efficiency of introducing a target substance into a cell, comprising (a) an actin acting substance. The present invention also provides a device and method using such a composition.

摘要翻译： 本发明提供能够提高在任何情况下难以引入（特别是转染）细胞的目标物质（例如DNA，多肽，糖或其复合物）的引入效率的方法。 特别地，本发明提供了用于提高将靶物质导入细胞的效率的组合物，其包含（a）肌动蛋白作用物质。 本发明还提供了使用这种组合物的装置和方法。

公开(公告)号：US20070244568A1

公开(公告)日：2007-10-18

申请号：US10584740

申请日：2004-12-24

申请人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa ,  Shigemitau Iwai ,  Takeyoshi Ota ,  Jun Miyake ,  Masayuki Hara ,  Masakazu Furuta ,  Eiichiro Uchimura

发明人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa ,  Shigemitau Iwai ,  Takeyoshi Ota ,  Jun Miyake ,  Masayuki Hara ,  Masakazu Furuta ,  Eiichiro Uchimura

IPC分类号： A61F2/02

CPC分类号： A61L27/3683 ,  A61L27/3604

摘要： Decellularization of tissue by means of an amphipathic solvent a well-established practice. However, situations exist where the provision of enhanced decellularization is preferred. There is a demand for treating methods for coping with such situations. Thus, it is intended to provide a method for enhancing decellularization. The method comprises not only the immersing of a tissue in a solution containing an amphiphilic molecule in non-micellar form (for example, 1,2-epoxide polymer) but also performing a radical reaction (for example, treatment selected from the group consisting of exposure to gamma-ray irradiation, ultraviolet irradiation, a free radical supply source, ultrasonication, electron beam irradiation, and X-ray irradiation).

摘要翻译： 通过两性溶剂去除细胞组织，这是一个很好的做法。 然而，存在提供增强脱细胞化优先的情况。 需要处理这种情况的方法。 因此，旨在提供一种增强脱细胞化的方法。 该方法不仅包括将组织浸入含有非胶束形式的两亲分子（例如1,2-环氧化物聚合物）的溶液中，而且还进行自由基反应（例如，选自由 暴露于γ射线照射，紫外线照射，自由基供应源，超声波处理，电子束照射和X射线照射）。

公开(公告)号：US20050256588A1

公开(公告)日：2005-11-17

申请号：US10519814

申请日：2003-06-27

申请人： Yoshiki Sawa ,  Satoshi Taketani ,  Shigemitsu Iwai ,  Hikaru Matsuda ,  Masayuki Hara ,  Eiichiro Uchimura ,  Jun Miyake

发明人： Yoshiki Sawa ,  Satoshi Taketani ,  Shigemitsu Iwai ,  Hikaru Matsuda ,  Masayuki Hara ,  Eiichiro Uchimura ,  Jun Miyake

IPC分类号： A61K35/12 ,  A61L27/00 ,  A61L27/36 ,  A61L27/38 ,  C12N5/071 ,  A61F2/02

CPC分类号： C12N5/0697 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L27/3804 ,  A61L2430/40 ,  C12N5/0691

摘要： An objective of the present invention is to overcome a problem that there is an inverse relationship between the decellularization rate and the strength of tissue. This problem was solved by immersing tissue in a solution containing a non-micellar amphipathic molecule (e.g., a 1,2-epoxide polymer). Thus, the present invention provides decellularized tissue, in which the cell survival rate of the tissue is less than a level at which calcification or an immune reaction is elicited in an organism and the tissue damage rate of the tissue is suppressed to a level which permits clinical applications. Tissue prepared by the above-described treatment preferably retains a certain level of tissue strength. Further, the tissue of the present invention has an effect of performing cell replacement.

摘要翻译： 本发明的目的是克服脱细胞率与组织强度之间存在反比关系的问题。 通过将组织浸入含有非胶束两亲性分子（例如1,2-环氧化物聚合物）的溶液中来解决该问题。 因此，本发明提供脱细胞组织，其中组织的细胞存活率小于在生物体中引起钙化或免疫反应的水平，并且组织的组织损伤率被抑制到允许 临床应用。 通过上述处理制备的组织优选保持一定水平的组织强度。 此外，本发明的组织具有进行细胞置换的效果。