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    Composition and Method for Elevating Gene Transfer Efficiency
    3.
    发明申请
    Composition and Method for Elevating Gene Transfer Efficiency 审中-公开
    提高基因转移效率的组成和方法

    公开(公告)号：US20080038824A1

    公开(公告)日：2008-02-14

    申请号：US10587776

    申请日：2005-01-27

    申请人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

    发明人： Masato Miyake ,  Tomohiro Yoshikawa ,  Eiichiro Uchimura ,  Jun Miyake

    IPC分类号： C12N15/00 ,  C07K14/00 ,  C07K16/00 ,  C07K16/28

    CPC分类号： C12N15/87 ,  C07K16/2842

    摘要： It is intended to provide a method whereby transfer efficiency can be improved under any conditions in the case of transferring (in particular, transfecting) a target substance that cannot easily be transferred into cells (for example, a DNA, a polypeptide, a sugar, a complex thereof or the like). Namely, a composition for elevating the transfer efficiency of a target substance into cells which contains a cell adhesion-related factor, and a device and a method using the composition.

    摘要翻译： 旨在提供一种方法，其可以在转移（特别是转染）不容易转移到细胞中的目标物质（例如DNA，多肽，糖， 其复合物等）。 即，将目标物质的转移效率提高为含有细胞粘附相关因子的细胞的组合物，以及使用该组合物的装置和方法。

    Method of forming a structure having multiple cell layers
    5.
    发明授权
    Method of forming a structure having multiple cell layers 失效
    形成具有多个单元层的结构的方法

    公开(公告)号：US06821107B1

    公开(公告)日：2004-11-23

    申请号：US09699133

    申请日：2000-10-27

    申请人： Masayuki Hara ,  Jun Miyake ,  Ayako Yamaki

    发明人： Masayuki Hara ,  Jun Miyake ,  Ayako Yamaki

    IPC分类号： C12N506

    CPC分类号： C12N5/0062 ,  C12N5/0068 ,  C12N2533/54 ,  C12N2533/74

    摘要： Multiple cell layers are formed with one cell layer formed on another cell layer. A carrier is provided having an alginate gel layer formed on a porous membrane. An extracellular matrix component gel layer or extracellular matrix component sponge layer may be formed on the alginate gel layer. A cell layer is formed on the alginate gel layer, or the extracellular matrix component gel layer or extracellular matrix component sponge layer. The alginate gel layer is solubilized such as with a chelating agent to exfoliate the cell layer from the porous membrane, and the exfoliated cell layer is placed on another cell layer on a carrier. The number of cell layers formed on each other may be 1-10, preferably 1-5, and more preferably 1-3.

    摘要翻译： 形成多个单元层，其中一个单元层形成在另一个单元层上。 提供了在多孔膜上形成藻酸盐凝胶层的载体。 可以在藻酸盐凝胶层上形成细胞外基质组分凝胶层或细胞外基质组分海绵层。 在藻酸盐凝胶层或细胞外基质组分凝胶层或细胞外基质组分海绵层上形成细胞层。 藻酸盐凝胶层例如用螯合剂溶解以从多孔膜剥离细胞层，并将剥离的细胞层置于载体上的另一个细胞层上。 彼此形成的电池层的数量可以为1-10，优选为1-5，更优选为1-3。

    Methods and systems for analyzing a network of biological functions
    6.
    发明申请
    Methods and systems for analyzing a network of biological functions 审中-公开
    分析生物功能网络的方法和系统

    公开(公告)号：US20070174009A1

    公开(公告)日：2007-07-26

    申请号：US11262554

    申请日：2005-10-28

    申请人： Masato Miyake ,  Tomohiro Yoshikawa ,  Takanori Ueda ,  Jun Miyake

    发明人： Masato Miyake ,  Tomohiro Yoshikawa ,  Takanori Ueda ,  Jun Miyake

    IPC分类号： G06F19/00

    CPC分类号： G16B5/00 ,  G16B20/00

    摘要： The present invention provides a method and system for analyzing a network of biological functions, such as transcriptional factors, structural genes, cellular markers, cell surface markers, cell shapes, organelle shapes, cell mobility, enzyme activities, metabolite concentrations, and localization of cellular components, in a biological entity such as a cell. Particularly, an object of the present invention is to provide a system and method for presenting biological information in a global manner without modification where the cell is considered a complex system.

    摘要翻译： 本发明提供了用于分析转录因子，结构基因，细胞标志物，细胞表面标志物，细胞形状，细胞器形状，细胞迁移率，酶活性，代谢物浓度和细胞定位的生物学功能网络的方法和系统 组分，在诸如细胞的生物实体中。 特别地，本发明的目的是提供一种用于以全局方式呈现生物信息的系统和方法，而不改变该细胞被认为是复杂的系统。

    Semiconductor memory device
    8.
    发明授权
    Semiconductor memory device 失效

    公开(公告)号：US5444665A

    公开(公告)日：1995-08-22

    申请号：US254416

    申请日：1994-06-06

    申请人： Yasunori Yamaguchi ,  Jun Miyake

    发明人： Yasunori Yamaguchi ,  Jun Miyake

    IPC分类号： G11C7/00 ,  G11C7/10 ,  H04N5/907 ,  G11C8/00

    CPC分类号： H04N5/907 ,  G11C7/00 ,  G11C7/1006

    摘要： A semiconductor memory device is provided with a memory portion, a logical operation circuit which receives the data signal read out from such memory portion and the input data signal to form data to be offered to such memory portion, and a gate circuit. In case a data input operation is required which eliminates the logical operation, the input data signal is fed not via the logical operation circuit, but via the gate circuit directly to the memory portion. The semiconductor memory device constructed as above permits a high speed operation and is suited for use as the memory for image processing.

    SMART METER AND METER READING SYSTEM
    9.
    发明申请
    SMART METER AND METER READING SYSTEM 审中-公开
    智能仪表和仪表读取系统

    公开(公告)号：US20120137126A1

    公开(公告)日：2012-05-31

    申请号：US13305041

    申请日：2011-11-28

    申请人： Kazunari MATSUOKA ,  Jun Miyake ,  Makoto Sato

    发明人： Kazunari MATSUOKA ,  Jun Miyake ,  Makoto Sato

    IPC分类号： H04L9/00

    CPC分类号： H04L9/0897 ,  H04L9/0838 ,  H04L9/3263 ,  H04L9/3271 ,  H04L63/061 ,  H04L63/0823 ,  Y04S40/24

    摘要： The present invention provides a smart meter for use in automatic meter reading of power, gas, and the like, preventing falsification of a program and data and assuring security in a communication path. A smart meter has: a data processor receiving a measurement signal according to a use amount, computing meter read data, and performing communication control by a communication unit coupled to a network; and a secure processor having tamper resistance for internally held information and performing secure authenticating process for a remote access. The data processor encrypts computed meter read data with a public key unique to the smart meter and supplies the encrypted data to the secure processor. The secure processor decrypts the encrypted meter read data with the secret key unique to the smart meter and stores the decrypted or encrypted meter read data into a nonvolatile storage region.

    摘要翻译： 本发明提供一种用于电力，气体等的自动抄表的智能电表，防止程序和数据的伪造，并确保通信路径的安全性。 智能电表具有：数据处理器，根据使用量接收测量信号，计算仪表读取数据，以及通过耦合到网络的通信单元执行通信控制; 以及具有用于内部保持的信息的防篡改的安全处理器，并执行用于远程访问的安全认证处理。 数据处理器使用智能电表独有的公共密钥加密计算的电表读取数据，并将加密的数据提供给安全处理器。 安全处理器使用智能电表特有的秘密密钥解密加密的电表读取数据，并将解密或加密的电表读取数据存储到非易失性存储区域中。

    Digital cell
    10.
    发明授权
    Digital cell 失效
    数字电池

    公开(公告)号：US07747390B2

    公开(公告)日：2010-06-29

    申请号：US10562469

    申请日：2004-06-25

    申请人： Masato Miyake ,  Tomohiro Yoshikawa ,  Jun Miyake

    发明人： Masato Miyake ,  Tomohiro Yoshikawa ,  Jun Miyake

    IPC分类号： G06F19/00 ,  G06F15/00

    CPC分类号： G01N33/5005 ,  C12M41/48

    摘要： It is intended to provide a method and system for carrying out data production with respect to the actual status of cells as a profile. It is also intended to provide a system and method for presenting time-lapse and/or real-time information of cell interior directly, or as it is, from the viewpoint of a complex system. It is further intended to provide a method of presenting a digital cell. Thus, there is provided a method of producing profile data relating to cell information, comprising the step (a) of arranging cells in an immobilized form on a support and the step (b) of monitoring over time biological factors on or inside the cells or an aggregate thereof, thereby producing profile data for the cells. Furthermore, there is provided a method of producing a digital cell, comprising procuring experimental data by the use of the above method.

    摘要翻译： 旨在提供一种用于执行关于作为简档的单元的实际状态的数据生成的方法和系统。 本发明还旨在提供一种从复杂系统的观点直接或者原样呈现小区内部的延时和/或实时信息的系统和方法。 还旨在提供呈现数字单元的方法。 因此，提供了一种产生与细胞信息有关的分布数据的方法，其包括将固定化形式的细胞排列在支持体上的步骤（a）和步骤（b） 其聚合，从而产生细胞的轮廓数据。 此外，提供了一种制造数字单元的方法，包括通过使用上述方法获得实验数据。