利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

7 条记录 (耗时 0.015 秒)

    Replication stable and RNase resistant chimeras of pestivirus with insertion in 3′ nontranslated region (3′NTR)
    2.
    发明授权
    Replication stable and RNase resistant chimeras of pestivirus with insertion in 3′ nontranslated region (3′NTR) 有权
    具有插入3'非翻译区（3'NTR）的瘟病毒复制稳定型和RNA酶抗性嵌合体

    公开(公告)号：US08986673B2

    公开(公告)日：2015-03-24

    申请号：US13470148

    申请日：2012-05-11

    申请人： Erhard Ralf Schoenbrunner ,  Sven-Erik Behrens

    发明人： Erhard Ralf Schoenbrunner ,  Sven-Erik Behrens

    IPC分类号： A01N63/00 ,  C12N15/00 ,  C07H21/02 ,  C12N7/00 ,  C12N15/86

    CPC分类号： C12N7/00 ,  C07H21/02 ,  C12N15/86 ,  C12N2770/24211 ,  C12N2770/24222 ,  C12N2770/24311 ,  C12N2770/24322

    摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.

    摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。

    Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
    3.
    发明申请
    Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR) 有权
    3'非翻译区插入的瘟病毒复制稳定型和RNA酶抗性嵌合体（3'NTR）

    公开(公告)号：US20120231539A1

    公开(公告)日：2012-09-13

    申请号：US13470148

    申请日：2012-05-11

    申请人： Erhard Ralf SCHOENBRUNNER ,  Sven-Erik Behrens

    发明人： Erhard Ralf SCHOENBRUNNER ,  Sven-Erik Behrens

    IPC分类号： C12N15/63 ,  C12N5/10

    CPC分类号： C12N7/00 ,  C07H21/02 ,  C12N15/86 ,  C12N2770/24211 ,  C12N2770/24222 ,  C12N2770/24311 ,  C12N2770/24322

    摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.

    摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。

    Controls For Detecting Methicillin Resistant Staphylococcus Aureus (MRSA)
    4.
    发明申请
    Controls For Detecting Methicillin Resistant Staphylococcus Aureus (MRSA) 审中-公开
    用于检测耐甲氧西林金黄色葡萄球菌（MRSA）的对照

    公开(公告)号：US20090197275A1

    公开(公告)日：2009-08-06

    申请号：US12367432

    申请日：2009-02-06

    申请人： Erhard Ralf Schoenbrunner ,  Alana Benn ,  Jerry Boonyarantanakornkit ,  Mona Shahbazian

    发明人： Erhard Ralf Schoenbrunner ,  Alana Benn ,  Jerry Boonyarantanakornkit ,  Mona Shahbazian

    IPC分类号： C12Q1/68 ,  C12N1/20

    CPC分类号： C12N1/20

    摘要： The invention relates to the quality control of Staphylococcus aureus testing using nucleic acid amplification-based detection assays. A Staphylococcus aureus control containing a quantified amount of the microorganism with high reproducibility across vials and which is used to calibrate, validate, or verify the performance of an MRSA detection assay and methods to test patient samples together with a control. Disclosed are specific Staphylococcus aureus strains that have a phenotype demonstrating reduced aggregation and increased consistency by Real-Time PCR compared to current Staphylococcus aureus strains used as external controls. Also disclosed is a process for increasing the reproducibility of Staphylococcus aureus strains that do not exhibit a non-aggregating phenotype.

    摘要翻译： 本发明涉及使用基于核酸扩增的检测测定法测定金黄色葡萄球菌的质量控制。 金黄色葡萄球菌对照，其含有定量的微生物，在小瓶之间具有高重现性，并且用于校准，验证或验证MRSA检测测定的性能以及与对照一起测试患者样品的方法。 公开了具有表型的表达型的金黄色葡萄球菌菌株，与用作外部对照的当前金黄色葡萄球菌菌株相比，通过实时PCR显示出降低的聚集和增加的一致性。 还公开了增加不显示非聚集表型的金黄色葡萄球菌菌株的再现性的方法。

    Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
    6.
    发明申请
    Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR) 审中-公开
    3'非翻译区插入的瘟病毒复制稳定型和RNA酶抗性嵌合体（3'NTR）

    公开(公告)号：US20100129902A1

    公开(公告)日：2010-05-27

    申请号：US12277282

    申请日：2008-11-24

    申请人： Erhard Ralf Schoenbrunner ,  Sven-Erik Behrens

    发明人： Erhard Ralf Schoenbrunner ,  Sven-Erik Behrens

    IPC分类号： C12N5/06 ,  C07H21/02 ,  C12N15/63

    CPC分类号： C12N7/00 ,  C07H21/02 ,  C12N15/86 ,  C12N2770/24211 ,  C12N2770/24222 ,  C12N2770/24311 ,  C12N2770/24322

    摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.

    摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。