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11 条记录 (耗时 0.033 秒)

    Methods for Identifying Nucleotides of Interest in Target Polynucleotides
    1.
    发明申请
    Methods for Identifying Nucleotides of Interest in Target Polynucleotides 审中-公开
    鉴定目的多核苷酸感兴趣核苷酸的方法

    公开(公告)号：US20080248469A1

    公开(公告)日：2008-10-09

    申请号：US11838639

    申请日：2007-08-14

    申请人： Eugene G. Spier

    发明人： Eugene G. Spier

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686

    摘要： In some embodiments, the present teachings provide a method of identifying a nucleotide of interest in a target polynucleotide. In some embodiments, the method can comprise forming an amplification strand, wherein the amplification strand comprises a hairpinning end region; hybridizing the hairpinning end region of the amplification strand with a hairpinning region of a target polynucleotide, to form a self-complementary amplification product. After performing an extension reaction, wherein the hairpinning end region of the amplification strand is extended, an extended reaction product is formed. Detection of the extended reaction product can result in the identification of a nucleotide of interest in the target polynucleotide. Additional methods, as well as kits, are also provided.

    摘要翻译： 在一些实施方案中，本发明提供了鉴定靶多核苷酸中所关注的核苷酸的方法。 在一些实施方案中，所述方法可以包括形成扩增链，其中所述扩增链包含发夹末端区; 将扩增链的发夹末端区域与靶多核苷酸的发夹区域杂交，形成自身互补扩增产物。 进行延伸反应后，扩增链的发夹末端区延长，形成延伸的反应产物。 延长的反应产物的检测可导致目标多核苷酸中目的核苷酸的鉴定。 还提供了其他方法以及套件。

    MicroRNA and Messenger RNA Detection On Arrays
    2.
    发明申请
    MicroRNA and Messenger RNA Detection On Arrays 审中-公开
    微阵列和信使RNA检测阵列

    公开(公告)号：US20100209932A1

    公开(公告)日：2010-08-19

    申请号：US12706627

    申请日：2010-02-16

    申请人： EUGENE G. SPIER

    发明人： EUGENE G. SPIER

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/143 ,  C12Q2525/207 ,  C12Q2565/501

    摘要： The present teachings provide methods for reverse transcribing, and detecting, a plurality of small nucleic acids such as micro RNAs, from the same reaction mixture as a plurality of messenger RNAs. High levels of multiplexing are provided by the use of a plurality of zip-coded stem-loop reverse transcription primers, along with an oligo-dT-promoter-containing reverse transcription primer, in the same reverse transcription reaction mixture. The resulting products can be amplified in an in vitro transcription reaction, and detected on a solid support such as an array. The present teachings also provide compositions, kits, and devices for performing and detecting the reverse transcription reactions described herein.

    摘要翻译： 本教导提供了与多个信使RNA相同的反应混合物逆转录和检测多个小核酸如微RNA的方法。 通过在相同的逆转录反应混合物中使用多个zip编码的茎环逆转录引物以及含有含有dT启动子的逆转录引物提供高水平的复用。 所得产物可以在体外转录反应中扩增，并在固体支持物如阵列上检测。 本教导还提供用于进行和检测本文所述的逆转录反应的组合物，试剂盒和装置。

    Double-ligation Method for Haplotype and Large-scale Polymorphism Detection
    4.
    发明申请
    Double-ligation Method for Haplotype and Large-scale Polymorphism Detection 审中-公开
    单倍型和大规​​模多态性检测的双重连接方法

    公开(公告)号：US20070196849A1

    公开(公告)日：2007-08-23

    申请号：US11677987

    申请日：2007-02-22

    申请人： Eugene G. Spier

    发明人： Eugene G. Spier

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2525/301 ,  C12Q2521/501

    摘要： The present teachings provide methods, compositions, and kits for querying the identity of a target polynucleotide strand comprising. In some embodiments, the present teachings provide a method comprising forming a reaction complex comprising the target polynucleotide strand hybridized to an upstream probe, a middle probe, and a downstream probe, wherein the middle probe comprises, A) a first target specific portion, B) a second target specific portion, C) a non-target specific portion, wherein the non-target specific portion is located between the first target specific portion and the second target specific portion, wherein the downstream probe comprises a 5′ end that is adjacent with the 3′ end of the middle probe, wherein the upstream probe comprises a 3′ end that is adjacent with the 5′ end of the middle probe; ligating the upstream probe to the middle probe and the middle probe to the downstream probe to form a ligation product; detecting the ligation product; and, determining the identity of the target polynucleotide strand.

    摘要翻译： 本教导提供了用于查询靶多核苷酸链的身份的方法，组合物和试剂盒，其包含。 在一些实施方案中，本发明提供了一种方法，包括形成包含与上游探针，中间探针和下游探针杂交的靶多核苷酸链的反应复合物，其中中间探针包含A）第一靶标特异性部分B ）第二目标特定部分，C）非目标特定部分，其中所述非靶特异部分位于所述第一靶特异部分和所述第二目标特异部分之间，其中所述下游探针包括相邻的5'端 中间探针的3'端，其中上游探针包括与中间探针的5'端相邻的3'端; 将上游探针与中间探针和中间探针连接到下游探针以形成连接产物; 检测结扎产物; 和确定靶多核苷酸链的身份。

    Methods, compositions, and kits for forming self-complementary polynucleotides
    8.
    发明授权
    Methods, compositions, and kits for forming self-complementary polynucleotides 有权
    用于形成自身互补多核苷酸的方法，组合物和试剂盒

    公开(公告)号：US07588896B2

    公开(公告)日：2009-09-15

    申请号：US11622991

    申请日：2007-01-12

    申请人： Eugene G. Spier

    发明人： Eugene G. Spier

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2561/125 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2525/155 ,  C12Q2521/501 ,  C12Q2525/307

    摘要： The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.

    摘要翻译： 本教导通常涉及用于检测靶多核苷酸序列的方法，试剂盒和组合物。 该教导还涉及产生自身互补多核苷酸产物的连接和扩增反应。 在一些实施方案中，使用导致形成自交联结合产物的探针进行连接反应。 将发夹接头连接到自交联结合产物上可以形成环结合产物。 在一些实施方案中，可以用滚环扩增扩增环结合产物。 环路连接产物的检测可以用于确定靶多核苷酸的身份。