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公开(公告)号:US20050181394A1
公开(公告)日:2005-08-18
申请号:US11006096
申请日:2004-12-07
申请人: Frank Steemers , Weihua Chang , Min-Jui Shen , Kevin Gunderson
发明人: Frank Steemers , Weihua Chang , Min-Jui Shen , Kevin Gunderson
CPC分类号: C12Q1/6827 , B01J2219/00432 , B01J2219/00524 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00648 , B01J2219/00662 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6816 , C12Q2565/549 , C12Q2565/537 , C12Q2565/513 , C12Q2537/143 , C12Q2535/125
摘要: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.
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2.
公开(公告)号:US20220033805A1
公开(公告)日:2022-02-03
申请号:US17276667
申请日:2020-03-02
申请人: Sanjay SRIVATSAN , Jose MCFALINE-FIGUEROA , Vijay RAMANI , Junyue CAO , Gregory BOOTH , Jay SHENDURE , Cole TRAPNELL , Frank STEEMERS , ILLUMINA, INC. , UNIVERSITY OF WASHINGTON
发明人: Sanjay Srivatsan , Jose McFaline-Figueroa , Vijay Ramani , Junyue Cao , Gregory Booth , Jay Shendure , Cole Trapnell , Frank J. Steemers
IPC分类号: C12N15/10
摘要: Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the method includes nuclear or cellular hashing which permits increased sample throughput and increased doublet detection at high collision rates. In one embodiment, the method includes normalization hashing which aids in estimating and removing technical noise in cell to cell variation and increases sensitivity and specificity.
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公开(公告)号:US20070122861A1
公开(公告)日:2007-05-31
申请号:US10221815
申请日:2001-03-14
申请人: David Walt , Caroline Schauer , Frank Steemers
发明人: David Walt , Caroline Schauer , Frank Steemers
摘要: The present invention provides a novel cross-reactive sensor system utilizing cross-reactive recognition elements. In the inventive system, each of said one or more cross-reactive recognition elements is capable of interacting with more than one species of liquid analyte of interest, whereby each of said one or more cross-reactive recognition elements reacts in a different manner with each of said one or more species of liquid analytes of interest to produce a detectable agent of each analyte of interest, whereby said detectable agent is analyzed and the information is processed for data acquisition and interpretation. In certain preferred embodiments, the detectable agent and/or change is detected directly, while in certain other preferred embodiments, the detectable agent and/or change is detected with the help of a transducing agent capable of relaying information about each detectable agent generated for each of said species of liquid analyte of interest, whereby said information is processed for data acquisition and interpretation. The present invention also provides method for the analysis of analytes comprising contacting one or more analytes with the inventive system described above.
摘要翻译: 本发明提供了一种利用交叉反应识别元件的新型交叉反应传感器系统。 在本发明的系统中,所述一个或多个交叉反应性识别元件中的每一个能够与多于一种的感兴趣的液体分析物相互作用,由此所述一个或多个交叉反应性识别元件中的每一个以不同的方式与每个 的所述一种或多种感兴趣的液体分析物种类以产生感兴趣的每种分析物的可检测试剂,由此分析所述可检测试剂,并且对所述信息进行处理以进行数据采集和解释。 在某些优选的实施方案中,直接检测可检测的试剂和/或变化,而在某些其它优选的实施方案中,可以借助于能够中继关于每个可产生的每个可检测试剂的信息的转导剂来检测可检测的试剂和/或变化 的所述物质的感兴趣的液体分析物,由此所述信息被处理用于数据采集和解释。 本发明还提供了用于分析分析物的方法,包括使一种或多种分析物与上述本发明的系统接触。
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公开(公告)号:US20120225787A1
公开(公告)日:2012-09-06
申请号:US13473378
申请日:2012-05-16
IPC分类号: C40B20/00
CPC分类号: C12Q1/6806 , C12Q1/6869 , C12Q2522/10 , C12Q2525/191
摘要: The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least one protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments.
摘要翻译: 本发明提供了一种用于制备和分析具有基本均匀尺寸的片段化多核苷酸序列群的方法。 该方法可以包括以下步骤:(a)将至少一种保护分子结合至至少一个多核苷酸序列; (b)切割所述至少一个多核苷酸序列以产生大致均匀尺寸的多个多核苷酸片段序列; (c)扩增多核苷酸片段; 和(d)确定多个多核苷酸片段的序列特征。
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公开(公告)号:US08202691B2
公开(公告)日:2012-06-19
申请号:US12357995
申请日:2009-01-22
CPC分类号: C12Q1/6806 , C12Q1/6869 , C12Q2522/10 , C12Q2525/191
摘要: The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least one protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments.
摘要翻译: 本发明提供了一种用于制备和分析具有基本均匀尺寸的片段化多核苷酸序列群的方法。 该方法可以包括以下步骤:(a)将至少一种保护分子结合至至少一个多核苷酸序列; (b)切割所述至少一个多核苷酸序列以产生大致均匀尺寸的多个多核苷酸片段序列; (c)扩增多核苷酸片段; 和(d)确定多个多核苷酸片段的序列特征。
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6.
公开(公告)号:US07670810B2
公开(公告)日:2010-03-02
申请号:US10872141
申请日:2004-06-17
申请人: Kevin Gunderson , Frank Steemers
发明人: Kevin Gunderson , Frank Steemers
IPC分类号: C12P19/34
CPC分类号: C12Q1/6827 , C12Q1/6816 , C12Q1/682 , C12Q1/6837 , C12Q1/6848 , C12Q2565/537 , C12Q2565/549 , C12Q2565/513 , C12Q2537/143 , C12Q2535/125 , C12Q2537/163 , C12Q2525/301 , C12Q2522/101
摘要: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.
摘要翻译: 本发明提供扩增基因组DNA以获得扩增的代表性基因组片段的方法。 进一步提供了获得所需复杂度的扩增基因组DNA表达的方法。 本发明还提供了用于同时检测扩增的代表性基因组片段群体的大量类型基因座的方法。 因此,该方法可用于在全基因组范围内对个体进行基因型分型。
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公开(公告)号:US20090191563A1
公开(公告)日:2009-07-30
申请号:US12357995
申请日:2009-01-22
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6806 , C12Q1/6869 , C12Q2522/10 , C12Q2525/191
摘要: The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least some protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments.
摘要翻译: 本发明提供了一种用于制备和分析具有基本均匀尺寸的片段化多核苷酸序列群的方法。 该方法可以包括以下步骤:(a)将至少一些保护分子结合至至少一个多核苷酸序列; (b)切割所述至少一个多核苷酸序列以产生大致均匀尺寸的多个多核苷酸片段序列; (c)扩增多核苷酸片段; 和(d)确定多个多核苷酸片段的序列特征。
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8.
公开(公告)号:US20050037393A1
公开(公告)日:2005-02-17
申请号:US10871513
申请日:2004-06-17
申请人: Kevin Gunderson , Frank Steemers
发明人: Kevin Gunderson , Frank Steemers
CPC分类号: C12Q1/6827 , C12Q1/6816 , C12Q1/682 , C12Q1/6837 , C12Q1/6848 , C12Q2565/537 , C12Q2565/549 , C12Q2565/513 , C12Q2537/143 , C12Q2535/125 , C12Q2537/163 , C12Q2525/301 , C12Q2522/101
摘要: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.
摘要翻译: 本发明提供扩增基因组DNA以获得扩增的代表性基因组片段的方法。 进一步提供了获得所需复杂度的扩增基因组DNA表达的方法。 本发明还提供了用于同时检测扩增的代表性基因组片段群体的大量类型基因座的方法。 因此,该方法可用于在全基因组范围内对个体进行基因型分型。
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9.
公开(公告)号:US09045796B2
公开(公告)日:2015-06-02
申请号:US10871513
申请日:2004-06-17
申请人: Kevin Gunderson , Frank Steemers
发明人: Kevin Gunderson , Frank Steemers
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6827 , C12Q1/6816 , C12Q1/682 , C12Q1/6837 , C12Q1/6848 , C12Q2565/537 , C12Q2565/549 , C12Q2565/513 , C12Q2537/143 , C12Q2535/125 , C12Q2537/163 , C12Q2525/301 , C12Q2522/101
摘要: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.
摘要翻译: 本发明提供扩增基因组DNA以获得扩增的代表性基因组片段的方法。 进一步提供了获得所需复杂度的扩增基因组DNA表达的方法。 本发明还提供了用于同时检测扩增的代表性基因组片段群体的大量类型基因座的方法。 因此,该方法可用于在全基因组范围内对个体进行基因分型。
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公开(公告)号:US08609341B2
公开(公告)日:2013-12-17
申请号:US13473378
申请日:2012-05-16
CPC分类号: C12Q1/6806 , C12Q1/6869 , C12Q2522/10 , C12Q2525/191
摘要: The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least one protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments.
摘要翻译: 本发明提供了一种用于制备和分析具有基本均匀尺寸的片段化多核苷酸序列群的方法。 该方法可以包括以下步骤:(a)将至少一种保护分子结合至至少一个多核苷酸序列; (b)切割所述至少一个多核苷酸序列以产生大致均匀尺寸的多个多核苷酸片段序列; (c)扩增多核苷酸片段; 和(d)确定多个多核苷酸片段的序列特征。
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