公开(公告)号：US20050208018A1

公开(公告)日：2005-09-22

申请号：US11040162

申请日：2005-01-20

申请人： Giovanna Tosato ,  Sandra Pike ,  Lei Yao

发明人： Giovanna Tosato ,  Sandra Pike ,  Lei Yao

IPC分类号： C07K14/47 ,  A61K38/20 ,  A61K38/18

CPC分类号： C07K14/4725

摘要： Methods for inhibiting endothelial cell growth and angiogenesis, and suppressing tumor growth using calreticulin, fragments of calreticulin and variants of calreticulin are provided. Such methods are useful for the treatment of cancer and diseases associated with unwanted angiogenesis, for example chronic retinal detachment.

摘要翻译： 提供了抑制内皮细胞生长和血管发生的方法，并且使用钙网蛋白，钙网蛋白的片段和钙网蛋白的变体来抑制肿瘤生长。 这样的方法可用于治疗与不需要的血管发生相关的癌症和疾病，例如慢性视网膜脱离。

公开(公告)号：US08420620B2

公开(公告)日：2013-04-16

申请号：US12864492

申请日：2009-01-23

申请人： Giovanna Tosato ,  Masashi Narazaki

发明人： Giovanna Tosato ,  Masashi Narazaki

IPC分类号： A01N43/04 ,  A61K31/715 ,  C07H21/02 ,  C07H1/00

CPC分类号： C07K19/00 ,  C07H11/00 ,  C07K14/705 ,  C07K2319/01 ,  C12N15/115

摘要： Disclosed is a hetero-bifunctional ligand for use in inducing internalization of a target receptor. The hetero-bifunctional ligand includes a target receptor-binding agent that specifically binds the target receptor linked to an internalizing receptor-binding agent that specifically binds to an internalizing receptor, where the two binding agents are non-identical. Also disclosed is a method of inducing the internalization of a target receptor on a cell. The method includes contacting a cell with a hetero-bifunctional ligand, where binding of the hetero-bifunctional ligand induces internalization of a target receptor of the cell. Also disclosed a method of treating a disease or condition associated with a target receptor using the disclosed hetero-bifunctional ligand and pharmaceutical compositions including a hetero-bifunctional ligand.

摘要翻译： 公开了用于诱导靶受体内化的异双功能配体。 异双功能配体包括特异性结合与特异性结合内化受体的内化受体结合剂连接的靶受体的靶受体结合剂，其中两种结合剂是不相同的。 还公开了诱导细胞上靶受体内化的方法。 该方法包括使细胞与异双功能配体接触，其中杂双功能配体的结合诱导细胞靶受体的内化。 还公开了使用所公开的异双功能配体和包含异双功能配体的药物组合物治疗与靶受体相关的疾病或病症的方法。

公开(公告)号：US20100297124A1

公开(公告)日：2010-11-25

申请号：US12864492

申请日：2009-01-23

申请人： Giovanna Tosato ,  Masashi Narazaki

发明人： Giovanna Tosato ,  Masashi Narazaki

IPC分类号： A61K39/395 ,  C07K16/28 ,  A61P35/00 ,  A61P31/18 ,  C07K14/475 ,  A61K38/18 ,  A61K31/737 ,  C08B37/00 ,  C07H21/04 ,  A61K31/7088 ,  C12N5/02

CPC分类号： C07K19/00 ,  C07H11/00 ,  C07K14/705 ,  C07K2319/01 ,  C12N15/115

摘要： Disclosed is a hetero-bifunctional ligand for use in inducing internalization of a target receptor. The hetero-bifunctional ligand includes a target receptor-binding agent that specifically binds the target receptor linked to an internalizing receptor-binding agent that specifically binds to an internalizing receptor, where the two binding agents are non-identical. Also disclosed is a method of inducing the internalization of a target receptor on a cell. The method includes contacting a cell with a hetero-bifunctional ligand, where binding of the hetero-bifunctional ligand induces internalization of a target receptor of the cell. Also disclosed a method of treating a disease or condition associated with a target receptor using the disclosed hetero-bifunctional ligand and pharmaceutical compositions including a hetero-bifunctional ligand.

摘要翻译： 公开了用于诱导靶受体内化的异双功能配体。 异双功能配体包括特异性结合与特异性结合内化受体的内化受体结合剂连接的靶受体的靶受体结合剂，其中两种结合剂是不相同的。 还公开了诱导细胞上靶受体内化的方法。 该方法包括使细胞与异双功能配体接触，其中杂双功能配体的结合诱导细胞靶受体的内化。 还公开了使用所公开的异双功能配体和包含异双功能配体的药物组合物治疗与靶受体相关的疾病或病症的方法。

公开(公告)号：US20090149380A1

公开(公告)日：2009-06-11

申请号：US12198810

申请日：2008-08-26

申请人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

发明人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

IPC分类号： A61K38/16 ,  C07K14/00 ,  C07K7/00 ,  C12N9/10 ,  C12N5/06 ,  A61K31/7088 ,  C12N15/11 ,  C12N15/00

CPC分类号： C07K14/4725 ,  A61K38/1709

摘要： Specific fragments of vasostatin are disclosed. These fragments are of use in methods of stimulating the proliferation or survival of a hematopoietic cell exposed to a chemotherapeutic agent or irradiation. Methods of stimulating the proliferation or survival of a hematopoietic cell using these fragments are also disclosed. In one embodiment, methods are disclosed for stimulating the growth or survival of a hematopoietic stem cell with a fragment of vasostatin, in the presence of a growth factor.

摘要翻译： 公开了血管抑素的特定片段。 这些片段可用于刺激暴露于化学治疗剂或照射的造血细胞的增殖或存活的方法中。 还公开了使用这些片段刺激造血细胞的增殖或存活的方法。 在一个实施方案中，公开了用于在生长因子存在下刺激造血干细胞与血管抑素片段的生长或存活的方法。

公开(公告)号：US07432236B2

公开(公告)日：2008-10-07

申请号：US10405588

申请日：2003-04-01

申请人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

发明人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

IPC分类号： A61K38/00

CPC分类号： C07K14/4725 ,  A61K38/1709

摘要： Specific fragments of vasostatin are disclosed. Also disclosed is a method of stimulating the proliferation or survival of a hematopoietic cell exposed to a chemotherapeutic agent or irradiation using these fragments. A method of stimulating the proliferation or survival of a hematopoietic cell is also disclosed. In one embodiment, the method is disclosed for stimulating the growth or survival of a hematopoietic stem cell with a fragment of vasostatin, in the presence of a growth factor.

摘要翻译： 公开了血管抑素的特定片段。 还公开了刺激暴露于化学治疗剂或使用这些片段的照射的造血细胞的增殖或存活的方法。 还公开了刺激造血细胞增殖或存活的方法。 在一个实施方案中，公开了用于在生长因子存在下刺激造血干细胞与血管抑素片段的生长或存活的方法。

公开(公告)号：US07374756B2

公开(公告)日：2008-05-20

申请号：US11803732

申请日：2007-05-14

申请人： Yoshiyasu Aoki ,  Giovanna Tosato

发明人： Yoshiyasu Aoki ,  Giovanna Tosato

IPC分类号： A61K39/395

CPC分类号： G01N33/56994 ,  A61K2039/505 ,  C07K16/085 ,  C07K16/248 ,  C12Q1/705 ,  G01N33/56983 ,  G01N33/6869 ,  G01N2333/03 ,  G01N2333/5412 ,  G01N2469/10 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2800/52 ,  C12Q2563/131

摘要： A specific binding agent is provided, wherein the specific binding agent specifically binds Kaposi's sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), and the specific binding agent neutralizes an activity of vIL-6. In one embodiment, the specific binding agent is an antibody. Methods are provided for using a specific binding agent that binds vIL-6, and neutralizes a biological activity of vIL-6. Methods of treatment for a KSHV-associated disorder are also provided. Methods for diagnosing a KSHV-associated disorder are provided, as are kits that include a specific binding agent of the invention. A method is also provided for testing an agent for effectiveness in treating a KSHV-associated disorder. The method includes incubating the agent with a cell free system comprising a vIL-6 receptor component and vIL-6, and comparing the binding of vIL-6 and the receptor component in the presence of the agent to binding of vIL-6 to the receptor component in the absence of the agent. A decrease in the binding of vIL-6 to the receptor component in the presence of the agent indicates that the agent is effective for treating the KSHV-associated disorder.

摘要翻译： 提供特异性结合剂，其中特异性结合剂特异性结合卡波西肉瘤相关疱疹病毒（KSHV）白细胞介素-6（vIL-6），特异性结合剂中和vIL-6的活性。 在一个实施方案中，特异性结合剂是抗体。 提供了使用结合vIL-6的特异性结合剂并中和vIL-6的生物学活性的方法。 还提供了KSHV相关疾病的治疗方法。 提供了用于诊断KSHV相关疾病的方法，以及包括本发明的特异性结合剂的试剂盒。 还提供了用于测试试剂以治疗KSHV相关病症的有效性的方法。 该方法包括将试剂与包含vIL-6受体组分和vIL-6的无细胞系统一起孵育，并比较在试剂存在下vIL-6和受体组分与vIL-6与受体结合的结合 组件在没有代理的情况下。 在试剂存在下，vIL-6与受体成分的结合减少表明该试剂对于治疗KSHV相关病症是有效的。

公开(公告)号：US07235365B2

公开(公告)日：2007-06-26

申请号：US11493687

申请日：2006-07-25

申请人： Yoshiyasu Aoki ,  Giovanna Tosato

发明人： Yoshiyasu Aoki ,  Giovanna Tosato

IPC分类号： C12Q1/68

CPC分类号： G01N33/56994 ,  A61K2039/505 ,  C07K16/085 ,  C07K16/248 ,  C12Q1/705 ,  G01N33/56983 ,  G01N33/6869 ,  G01N2333/03 ,  G01N2333/5412 ,  G01N2469/10 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2800/52 ,  C12Q2563/131

摘要： A specific binding agent is provided, wherein the specific binding agent specifically binds Kaposi's sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), and the specific binding agent neutralizes an activity of vIL-6. In one embodiment, the specific binding agent is an antibody. Methods are provided for using a specific binding agent that binds vIL-6, and neutralizes a biological activity of vIL-6. Methods of treatment for a KSHV-associated disorder are also provided. Methods for diagnosing a KSHV-associated disorder are provided, as are kits that include a specific binding agent of the invention. A method is also provided for testing an agent for effectiveness in treating a KSHV-associated disorder. The method includes incubating the agent with a cell free system comprising a vIL-6 receptor component and vIL-6, and comparing the binding of vIL-6 and the receptor component in the presence of the agent to binding of vIL-6 to the receptor component in the absence of the agent. A decrease in the binding of vIL-6 to the receptor component in the presence of the agent indicates that the agent is effective for treating the KSHV-associated disorder.

摘要翻译： 提供特异性结合剂，其中特异性结合剂特异性结合卡波西肉瘤相关疱疹病毒（KSHV）白细胞介素-6（vIL-6），特异性结合剂中和vIL-6的活性。 在一个实施方案中，特异性结合剂是抗体。 提供了使用结合vIL-6的特异性结合剂并中和vIL-6的生物学活性的方法。 还提供了KSHV相关疾病的治疗方法。 提供了用于诊断KSHV相关疾病的方法，以及包括本发明的特异性结合剂的试剂盒。 还提供了用于测试试剂以治疗KSHV相关病症的有效性的方法。 该方法包括将试剂与包含vIL-6受体组分和vIL-6的无细胞系统一起孵育，并比较在试剂存在下vIL-6和受体组分与vIL-6与受体结合的结合 组件在没有代理的情况下。 在试剂存在下，vIL-6与受体成分的结合减少表明该试剂对于治疗KSHV相关病症是有效的。

公开(公告)号：US06939547B2

公开(公告)日：2005-09-06

申请号：US10333121

申请日：2001-07-31

申请人： Yoshiyasu Aoki ,  Giovanna Tosato

发明人： Yoshiyasu Aoki ,  Giovanna Tosato

IPC分类号： A61P31/20 ,  A61P35/00 ,  C07K16/08 ,  C07K16/24 ,  C12Q1/70 ,  G01N33/569 ,  G01N33/68 ,  A61K39/245

CPC分类号： G01N33/56994 ,  A61K2039/505 ,  C07K16/085 ,  C07K16/248 ,  C12Q1/705 ,  G01N33/56983 ,  G01N33/6869 ,  G01N2333/03 ,  G01N2333/5412 ,  G01N2469/10 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2500/04 ,  G01N2800/52 ,  C12Q2563/131

摘要： A specific binding agent is provided, wherein the specific binding agent specifically binds Kaposi's sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), and the specific binding agent neutralizes an activity of vIL-6. In one embodiment, the specific binding agent is an antibody. Methods are provided for using a specific binding agent that binds vIL-6, and neutralizes a biological activity of vIL-6. Methods of treatment for a KSHV-associated disorder are also provided. Methods for diagnosing a KSHV-associated disorder are provided, as are kits that include a specific binding agent of the invention. A method is also provided for testing an agent for effectiveness in treating a KSHV-associated disorder. The method includes incubating the agent with a cell free system comprising a vIL-6 receptor component and vIL-6, and comparing the binding of vIL-6 and the receptor component in the presence of the agent to binding of vIL-6 to the receptor component in the absence of the agent. A decrease in the binding of vIL-6 to the receptor component in the presence of the agent indicates that the agent is effective for treating the KSHV-associated disorder.

摘要翻译： 提供特异性结合剂，其中特异性结合剂特异性结合卡波西肉瘤相关疱疹病毒（KSHV）白细胞介素-6（vIL-6），特异性结合剂中和vIL-6的活性。 在一个实施方案中，特异性结合剂是抗体。 提供了使用结合vIL-6的特异性结合剂并中和vIL-6的生物学活性的方法。 还提供了KSHV相关疾病的治疗方法。 提供了用于诊断KSHV相关疾病的方法，以及包括本发明的特异性结合剂的试剂盒。 还提供了用于测试试剂以治疗KSHV相关病症的有效性的方法。 该方法包括将试剂与包含vIL-6受体组分和vIL-6的无细胞系统一起孵育，并比较在试剂存在下vIL-6和受体组分与vIL-6与受体结合的结合 组件在没有代理的情况下。 在试剂存在下，vIL-6与受体成分的结合减少表明该试剂对于治疗KSHV相关病症是有效的。

公开(公告)号：US06596690B2

公开(公告)日：2003-07-22

申请号：US09828000

申请日：2001-04-06

申请人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

发明人： Giovanna Tosato ,  Sandra E. Pike ,  Lei Yao

IPC分类号： A61K3816

CPC分类号： C07K14/4725 ,  A61K38/1709

摘要： Specific fragments of vasostatin are disclosed. Also disclosed is a method of stimulating the proliferation or survival of a hematopoietic cell exposed to a chemotherapeutic agent or irradiation using these fragments. A method of stimulating the proliferation or survival of a hematopoietic cell is also disclosed. In one embodiment, the method is disclosed for stimulating the growth or survival of a hematopoietic stem cell with a fragment of vasostatin, in the presence of a growth factor.

摘要翻译： 公开了血管抑素的特定片段。 还公开了刺激暴露于化学治疗剂或使用这些片段的照射的造血细胞的增殖或存活的方法。 还公开了刺激造血细胞增殖或存活的方法。 在一个实施方案中，公开了用于在生长因子存在下刺激造血干细胞与血管抑素片段的生长或存活的方法。

公开(公告)号：US06509321B1

公开(公告)日：2003-01-21

申请号：US09672448

申请日：2000-09-29

申请人： Robert Yarchoan ,  James M. Pluda ,  Kathleen Wyvill ,  Jill Lietzau ,  Gene M. Shearer ,  Ellen Feigal ,  Giovanna Tosato ,  Richard Little ,  Matthew L. Sherman

发明人： Robert Yarchoan ,  James M. Pluda ,  Kathleen Wyvill ,  Jill Lietzau ,  Gene M. Shearer ,  Ellen Feigal ,  Giovanna Tosato ,  Richard Little ,  Matthew L. Sherman

IPC分类号： A01N4304

CPC分类号： A61K38/208 ,  A61K48/00

摘要： Methods are provided for using IL-12 to treat Kaposi's sarcoma (KS), particularly AIDS-associated KS.