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    Lock mass ions for use with derivatized peptides for de novo sequencing using tandem mass spectrometry
    2.
    发明申请
    Lock mass ions for use with derivatized peptides for de novo sequencing using tandem mass spectrometry 审中-公开
    使用串联质谱法将质子离子锁定用于衍生肽进行从头测序

    公开(公告)号：US20060014293A1

    公开(公告)日：2006-01-19

    申请号：US10938690

    申请日：2004-09-10

    申请人： Timothy Joyce ,  Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    发明人： Timothy Joyce ,  Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6851 ,  G01N33/6842 ,  G01N33/6848 ,  H01J49/0031 ,  Y10T436/24

    摘要： Multiple derivatization by chemical reactions of analytes for mass spectrometry is disclosed. The derivatizations enhance the use of MS techniques for analyzing protein samples, particularly when the sequence of a polypeptide is determined by tandem MS/MS. Accurate mass analysis techniques are described for use in sequencing polypeptides, together with the use of sequencing data in protein analysis. An apparatus and method for calibrating a mass spectrometer by internally introducing calibration masses at a post-source stage of the mass spectrometer is also provided. Lock mass ions mix with the derivatized polypeptide analyte ions prior to mass analysis.

    摘要翻译： 公开了通过化学反应进行质谱分析的多重衍生化。 衍生化增强了MS技术用于分析蛋白质样品的用途，特别是当通过串联MS / MS测定多肽的序列时。 描述了准确的质量分析技术用于测序多肽，以及在蛋白质分析中使用测序数据。 还提供了通过在质谱仪的后源阶段内部引入校准质量来校准质谱仪的装置和方法。 在质量分析之前，将质子离子与衍生的多肽分析物离子混合。

    Compositions, methods, systems, and kits for affinity purification
    3.
    发明申请
    Compositions, methods, systems, and kits for affinity purification 审中-公开
    用于亲和纯化的组合物，方法，系统和试剂盒

    公开(公告)号：US20070148721A1

    公开(公告)日：2007-06-28

    申请号：US11319885

    申请日：2005-12-28

    申请人： Gordon Nicol ,  Barry Boyes

    发明人： Gordon Nicol ,  Barry Boyes

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/6842 ,  B01J20/223 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3265 ,  B01J45/00 ,  B01J2220/54 ,  B01J2220/58 ,  C07K1/22

    摘要： The invention provides a method of separating proteins and peptides of a sample comprising contacting the sample with a Pd coordination compound. The Pd coordination compound binds to sulfur and/or nitrogen groups and thus is useful for purifying biomolecules comprising cysteine, methionine, histidine amino acids or derivatized amino acids/residues comprising sulfur or nitrogen groups which bind to coordination sites on the Pd coordination compounds. The invention also relates to methods, systems and kits for using the Pd coordination compound.

    摘要翻译： 本发明提供了分离样品的蛋白质和肽的方法，包括使样品与Pd配位化合物接触。 Pd配位化合物与硫和/或氮基团结合，因此可用于纯化包含半胱氨酸，甲硫氨酸，组氨酸氨基酸或包含与Pd配位化合物上的配位点结合的硫或氮基团的衍生氨基酸/残基的生物分子。 本发明还涉及使用Pd配位化合物的方法，系统和试剂盒。

    Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry
    4.
    发明申请
    Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry 失效
    使用串联质谱法进行从头测序的肽的序列衍生化

    公开(公告)号：US20060014210A1

    公开(公告)日：2006-01-19

    申请号：US10892870

    申请日：2004-07-16

    申请人： Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    发明人： Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    IPC分类号： G01N33/53 ,  G01N33/48 ,  C07K14/47 ,  G06F19/00

    CPC分类号： G01N33/6851 ,  G01N33/6842 ,  G01N33/6848

    摘要： Serial derivatization by chemical reactions of analytes for mass spectrometry is disclosed. The derivatizations enhance the use of MS techniques for analyzing protein samples, particularly when the sequence of a polypeptide is determined by tandem MS/MS. Accurate mass analysis techniques are described for use in sequencing polypeptides, together with the use of sequencing data in protein analysis.

    摘要翻译： 公开了通过化学反应进行质谱分析物的串行衍生化。 衍生化增强了MS技术用于分析蛋白质样品的用途，特别是当通过串联MS / MS测定多肽的序列时。 描述了准确的质量分析技术用于测序多肽，以及在蛋白质分析中使用测序数据。

    MALDI-MS analysis of nucleic acids bound to a surface
    6.
    发明申请
    MALDI-MS analysis of nucleic acids bound to a surface 审中-公开
    与表面结合的核酸的MALDI-MS分析

    公开(公告)号：US20050186579A1

    公开(公告)日：2005-08-25

    申请号：US10785337

    申请日：2004-02-23

    申请人： Douglas Dellinger ,  Barry Boyes ,  Gordon Nicol

    发明人： Douglas Dellinger ,  Barry Boyes ,  Gordon Nicol

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q2565/627

    摘要： A method of analyzing a polynucleotide using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The method includes obtaining the polynucleotide bound to a substrate via a linker moiety having a triaryl methyl linker group. The polynucleotide bound to the substrate is then contacted with a matrix material and analyzed by MALDI-MS. During the MALDI-MS analysis, laser radiation is directed at the matrix material, thereby exciting the matrix material and causing cleavage of the linker moiety. Ions generated as a result of this excitation and cleavage process are then analyzed to provide information about the polynucleotide.

    摘要翻译： 描述了使用基质辅助激光解吸/电离质谱法（MALDI-MS）分析多核苷酸的方法。 该方法包括通过具有三芳基甲基连接基团的接头部分获得与底物结合的多核苷酸。 然后将与底物结合的多核苷酸与基质材料接触并通过MALDI-MS分析。 在MALDI-MS分析期间，激光辐射被引导到基质材料，从而激发基质材料并导致连接体部分的裂解。 然后分析由于该激发和切割过程产生的离子以提供关于多核苷酸的信息。

    Methods, systems and devices for performing analytical protocols
    7.
    发明申请
    Methods, systems and devices for performing analytical protocols 审中-公开
    用于执行分析协议的方法，系统和设备

    公开(公告)号：US20050106740A1

    公开(公告)日：2005-05-19

    申请号：US10713761

    申请日：2003-11-13

    申请人： Barry Boyes ,  Nina Zolotarjova ,  Gordon Nicol

    发明人： Barry Boyes ,  Nina Zolotarjova ,  Gordon Nicol

    IPC分类号： C07K1/22 ,  C07K1/36 ,  G01N27/447 ,  G01N30/46 ,  G01N30/89 ,  G01N33/543 ,  G01N33/68 ,  G01N30/02

    CPC分类号： G01N33/543 ,  C07K1/22 ,  C07K1/36 ,  G01N27/44726 ,  G01N27/44747 ,  G01N27/44773 ,  G01N30/461 ,  B01D15/3804

    摘要： Methods, systems and device for performing analytical protocols are provided. Embodiments of the subject methods include sequentially contacting a sample with at least a first stationary phase and a second stationary phase under chromatographic conditions to at least determine the binding identity of the at least one constituent, where the specificity of the first stationary phase for at least one constituent present in the sample is at least uncertain and the specificity of the second stationary phase for the at least one constituent is certain. Also provided are systems and devices that include at least the first and second stationary phases. Kits for use in practicing the subject methods are also provided.

    摘要翻译： 提供了用于执行分析方案的方法，系统和设备。 本发明方法的实施方案包括在色谱条件下使样品与至少第一固定相和第二固定相顺序接触以至少确定至少一种成分的结合同一性，其中第一固定相的特异性至少为 样品中存在的一个成分至少是不确定的，并且对于至少一种成分的第二固定相的特异性是确定的。 还提供了至少包括第一和第二固定相的系统和装置。 还提供了用于实践主题方法的套件。

    Quality control method for array manufacture
    8.
    发明申请
    Quality control method for array manufacture 审中-公开
    阵列制造质量控制方法

    公开(公告)号：US20050186580A1

    公开(公告)日：2005-08-25

    申请号：US10785898

    申请日：2004-02-23

    申请人： Douglas Dellinger ,  Barry Boyes ,  Gordon Nicol

    发明人： Douglas Dellinger ,  Barry Boyes ,  Gordon Nicol

    IPC分类号： B01J19/00 ,  C12Q1/68 ,  C40B60/14

    CPC分类号： B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00662 ,  B01J2219/00693 ,  B01J2219/00707 ,  C12Q1/6837 ,  C40B60/14

    摘要： A method of analyzing an array during and/or after fabrication to obtain information relating to the quality of the array manufacturing process is described. The method includes providing an array of features on a substrate, wherein each feature has one or more polynucleotides bound to the substrate. At least one of the features of the provided array is a cleavable feature. The cleavable feature has one or more polynucleotides bound to the substrate via a cleavable linker. The cleavable feature is then contacted with a matrix material, and a MALDI-MS protocol is used to obtain information about the one or more polynucleotides of the cleavable feature. This information may then be used to evaluate the manufacturing process.

    摘要翻译： 描述了在制造期间和/或之后分析阵列以获得与阵列制造工艺的质量有关的信息的方法。 该方法包括在衬底上提供特征阵列，其中每个特征具有结合到衬底的一个或多个多核苷酸。 所提供的阵列的至少一个特征是可切割的特征。 可切割特征具有通过可切割连接子与底物结合的一个或多个多核苷酸。 然后将可切割特征与基质材料接触，并使用MALDI-MS方案获得关于可切割特征的一个或多个多核苷酸的信息。 然后可以使用该信息来评估制造过程。