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32 条记录 (耗时 0.024 秒)

    Lock mass ions for use with derivatized peptides for de novo sequencing using tandem mass spectrometry
    1.
    发明申请
    Lock mass ions for use with derivatized peptides for de novo sequencing using tandem mass spectrometry 审中-公开
    使用串联质谱法将质子离子锁定用于衍生肽进行从头测序

    公开(公告)号：US20060014293A1

    公开(公告)日：2006-01-19

    申请号：US10938690

    申请日：2004-09-10

    申请人： Timothy Joyce ,  Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    发明人： Timothy Joyce ,  Barry Boyes ,  Gordon Nicol ,  Hongbin Liu

    IPC分类号： G01N33/00

    CPC分类号： G01N33/6851 ,  G01N33/6842 ,  G01N33/6848 ,  H01J49/0031 ,  Y10T436/24

    摘要： Multiple derivatization by chemical reactions of analytes for mass spectrometry is disclosed. The derivatizations enhance the use of MS techniques for analyzing protein samples, particularly when the sequence of a polypeptide is determined by tandem MS/MS. Accurate mass analysis techniques are described for use in sequencing polypeptides, together with the use of sequencing data in protein analysis. An apparatus and method for calibrating a mass spectrometer by internally introducing calibration masses at a post-source stage of the mass spectrometer is also provided. Lock mass ions mix with the derivatized polypeptide analyte ions prior to mass analysis.

    摘要翻译： 公开了通过化学反应进行质谱分析的多重衍生化。 衍生化增强了MS技术用于分析蛋白质样品的用途，特别是当通过串联MS / MS测定多肽的序列时。 描述了准确的质量分析技术用于测序多肽，以及在蛋白质分析中使用测序数据。 还提供了通过在质谱仪的后源阶段内部引入校准质量来校准质谱仪的装置和方法。 在质量分析之前，将质子离子与衍生的多肽分析物离子混合。

    Nanowire target support and method
    2.
    发明申请
    Nanowire target support and method 失效
    纳米线目标支持和方法

    公开(公告)号：US20060097149A1

    公开(公告)日：2006-05-11

    申请号：US10971164

    申请日：2004-10-22

    申请人： Timothy Joyce

    发明人： Timothy Joyce

    IPC分类号： H01J49/00

    CPC分类号： H01J49/164 ,  H01J49/0418

    摘要： The invention provides an apparatus that produces analyte ions for detection by a detector. The apparatus includes a matrix based ion source having a nanowire target substrate for producing analyte ions, an ion transport system adjacent to the matrix based ion source for transporting analyte ions from the matrix based ion source; and an ion detector downstream from the ion transport system for detecting the analyte ions. The invention also provides a method for producing and detecting the analyte ions.

    摘要翻译： 本发明提供一种生成分析物离子以供检测器检测的装置。 该装置包括具有用于产生分析物离子的纳米线靶基质的基于基质的离子源，与基于基质的离子源相邻的离子传输系统，用于将基于离子源的离子源输送到分析物离子; 以及离子传感系统下游的用于检测分析物离子的离子检测器。 本发明还提供了一种用于产生和检测分析物离子的方法。

    Characterization of biopolymers by resonance tunneling and fluorescence quenching
    4.
    发明申请
    Characterization of biopolymers by resonance tunneling and fluorescence quenching 审中-公开
    通过共振隧道和荧光猝灭表征生物聚合物

    公开(公告)号：US20060019259A1

    公开(公告)日：2006-01-26

    申请号：US10898586

    申请日：2004-07-22

    申请人： Timothy Joyce

    发明人： Timothy Joyce

    IPC分类号： C12Q1/68 ,  C12M3/04

    CPC分类号： G01N33/6818 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2565/607

    摘要： The present invention provides a method and apparatus for determining the identity of a monomeric residue of a biopolymer. The apparatus comprises a substrate having a nanopore, a potential-producing element for producing a ramped potential across electrodes adjacent to the nanopore, and a quenchable excitable moiety adjacent to the nanopore. As a biopolymer passes through the nanopore, the identity of monomeric residues of a biopolymer may be determined by detecting changes in (a) current across the electrodes and (b) a signal of the quenchable excitable molecule. The subject method and apparatus find use in determining the identity of a plurality of monomeric residues of a biopolymer, and, as such, may be employed in a variety of diagnostic and research applications.

    摘要翻译： 本发明提供了用于测定生物聚合物的单体残基的身份的方法和装置。 该装置包括具有纳米孔的底物，用于在邻近纳米孔的电极之间产生斜坡电位的潜在产生元件和与该纳米孔相邻的可淬火的可兴奋部分。 当生物聚合物通过纳米孔时，生物聚合物的单体残基的身份可以通过检测（a）跨越电极的电流的变化和（b）可淬灭的可激发分子的信号来确定。 本发明的方法和装置用于确定生物聚合物的多个单体残基的身份，并且因此可用于多种诊断和研究应用。

    Apparatus and method for ion production enhancement
    7.
    发明申请
    Apparatus and method for ion production enhancement 有权
    用于离子生产增强的装置和方法

    公开(公告)号：US20050151090A1

    公开(公告)日：2005-07-14

    申请号：US11015190

    申请日：2004-12-16

    申请人： Jean-Luc Truche ,  Jian Bai ,  Timothy Joyce

    发明人： Jean-Luc Truche ,  Jian Bai ,  Timothy Joyce

    IPC分类号： H01J49/04 ,  H01J49/16 ,  H01J27/00

    CPC分类号： H01J49/164 ,  H01J49/0477

    摘要： The present invention relates to an apparatus and method for use with a mass spectrometer. The ion enhancement system of the present invention is used to direct a heated gas toward ions produced by a matrix based ion source and detected by a detector. The ion enhancement system is interposed between the ion source and the detector. The analyte ions that contact the heated gas are enhanced and an increased number of ions are more easily detected by a detector. The method of the invention comprises producing analyte ions from a matrix based ion source, enhancing the analyte ions with an ion enhancement system and detecting the enhanced analyte ions with a detector.

    摘要翻译： 本发明涉及一种与质谱仪一起使用的装置和方法。 本发明的离子增强系统用于将加热气体引导到由基于基质的离子源产生的离子并由检测器检测。 离子增强系统介于离子源和检测器之间。 接触加热气体的分析物离子增强，并且更多的离子更容易被检测器检测。 本发明的方法包括从基于基质的离子源产生分析物离子，用离子增强系统增强分析物离子并用检测器检测增强的分析物离子。

    Nanospray ionization device and method
    8.
    发明申请
    Nanospray ionization device and method 有权
    纳米喷雾电离装置及方法

    公开(公告)号：US20070023676A1

    公开(公告)日：2007-02-01

    申请号：US11171976

    申请日：2005-06-29

    申请人： Paul Goodley ,  Timothy Joyce ,  Jerry Dowell

    发明人： Paul Goodley ,  Timothy Joyce ,  Jerry Dowell

    IPC分类号： H01J27/00

    CPC分类号： H01J49/165 ,  H01J49/0468

    摘要： The present invention provides an apparatus and method for use with a mass spectrometry system. The invention provides an ion source for providing radiative heating to an ionization region. The ion source includes a nanospray ionization device for producing ions and a conduit adjacent to the ionization device for receiving ions from the ionization device. The conduit includes a conductive material for providing indirect radiative heating to the ionization region. Direct radiative heating may also be provided using a heater in the conduit. The ion source may be used separately or in conjunction with the mass spectrometry system. When used in conjunction with a mass spectrometry system a detector may also be employed down stream from the device. A method for desolvating an analyte using the device is also disclosed.

    摘要翻译： 本发明提供了一种与质谱系统一起使用的装置和方法。 本发明提供了一种用于向电离区域提供辐射加热的离子源。 离子源包括用于产生离子的纳米喷雾离子化装置和与电离装置相邻的导管，用于从离子化装置接收离子。 导管包括用于向电离区提供间接辐射加热的导电材料。 也可以使用导管中的加热器来提供直接辐射加热。 离子源可以单独使用或与质谱系统结合使用。 当与质谱系统结合使用时，也可以从装置下游使用检测器。 还公开了使用该装置对分析物进行脱溶剂的方法。

    Apparatus and method for ion production enhancement
    9.
    发明授权
    Apparatus and method for ion production enhancement 有权

    公开(公告)号：US07135689B2

    公开(公告)日：2006-11-14

    申请号：US11041118

    申请日：2005-01-21

    申请人： Jean-Luc Truche ,  Jian Bai ,  Timothy Joyce

    发明人： Jean-Luc Truche ,  Jian Bai ,  Timothy Joyce

    IPC分类号： H01J27/00

    CPC分类号： H01J49/164 ,  H01J49/0477

    摘要： The present invention relates to an apparatus and method for use with a mass spectrometer. The ion enhancement system of the present invention is used to direct a heated gas toward ions produced by a matrix based ion source and detected by a detector. The ion enhancement system is interposed between the ion source and the detector. The analyte ions that contact the heated gas are enhanced and an increased number of ions are more easily detected by a detector. The method of the invention comprises producing analyte ions from a matrix based ion source, enhancing the analyte ions with an ion enhancement system and detecting the enhanced analyte ions with a detector.