Potting for electronic components
    1.
    发明授权
    Potting for electronic components 有权
    电子组件灌封

    公开(公告)号:US08614266B2

    公开(公告)日:2013-12-24

    申请号:US13102029

    申请日:2011-05-05

    摘要: A potting material for an electronic component, an electronic component, and a process for positioning ferrites in an electronic material are disclosed. The potting material is formed by curing a mixture. The mixture includes an epoxy component, an organic amine hardener, a viscosity-controlling agent, and a silica. The potting material has a coefficient of thermal expansion between an inorganic ferrite coefficient of thermal expansion and an organic substrate coefficient of thermal expansion of the electronic component. The potting material includes a rigidity permitting via drilling by one or more of mechanical drilling and laser burning.

    摘要翻译: 公开了一种用于电子部件的灌封材料,电子部件以及将铁氧体定位在电子材料中的工艺。 灌封材料通过固化混合物形成。 该混合物包括环氧组分,有机胺硬化剂,粘度控制剂和二氧化硅。 封装材料具有无机铁氧体热膨胀系数与电子部件的有机基板热膨胀系数之间的热膨胀系数。 灌封材料包括通过一次或多次机械钻孔和激光燃烧钻孔所允许的刚度。

    Transposon end compositions and methods for modifying nucleic acids
    4.
    发明授权
    Transposon end compositions and methods for modifying nucleic acids 有权
    转座子末端组合物和修饰核酸的方法

    公开(公告)号:US09080211B2

    公开(公告)日:2015-07-14

    申请号:US12605337

    申请日:2009-10-24

    IPC分类号: C12Q1/68 C12P19/34 C12N15/10

    摘要: The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.).

    摘要翻译: 本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法,组合物和试剂盒,然后使用DNA聚合酶产生5'-和3'标记 单链DNA片段,而不进行PCR扩增反应,其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列,3'-末端的第二个标签显示出 与第一个标签展示的序列不同的序列。 该方法可用于产生用于各种过程的5'-和3'标记的DNA片段,包括环境样品中DNA的宏基因组分析过程,DNA拷贝数变异(CNV)分析和比较基因组测序( CGS),包括大规模并行DNA测序(所谓的“下一代测序”)。

    POTTING FOR ELECTRONIC COMPONENTS
    7.
    发明申请
    POTTING FOR ELECTRONIC COMPONENTS 有权
    电子元器件

    公开(公告)号:US20110272191A1

    公开(公告)日:2011-11-10

    申请号:US13102029

    申请日:2011-05-05

    摘要: A potting material for an electronic component, an electronic component, and a process for positioning ferrites in an electronic material are disclosed. The potting material is formed by curing a mixture. The mixture includes an epoxy component, an organic amine hardener, a viscosity-controlling agent, and a silica. The potting material has a coefficient of thermal expansion between an inorganic ferrite coefficient of thermal expansion and an organic substrate coefficient of thermal expansion of the electronic component. The potting material includes a rigidity permitting via drilling by one or more of mechanical drilling and laser burning.

    摘要翻译: 公开了一种用于电子部件的灌封材料,电子部件以及将铁氧体定位在电子材料中的工艺。 灌封材料通过固化混合物形成。 该混合物包括环氧组分,有机胺硬化剂,粘度控制剂和二氧化硅。 封装材料具有无机铁氧体热膨胀系数与电子部件的有机基板热膨胀系数之间的热膨胀系数。 灌封材料包括通过一次或多次机械钻孔和激光燃烧钻孔所允许的刚度。

    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS
    9.
    发明申请
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIZING NUCLEIC ACIDS

    公开(公告)号:US20140162897A1

    公开(公告)日:2014-06-12

    申请号:US14148463

    申请日:2014-01-06

    IPC分类号: C12Q1/68

    摘要: The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.)

    摘要翻译: 本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法,组合物和试剂盒,然后使用DNA聚合酶产生5'-和3'标记 单链DNA片段,而不进行PCR扩增反应,其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列,3'-末端的第二个标签显示出 与第一个标签展示的序列不同的序列。 该方法可用于产生用于各种过程的5'-和3'标记的DNA片段,包括环境样品中DNA的宏基因组分析过程,DNA拷贝数变异(CNV)分析和比较基因组测序( CGS),包括大规模并行DNA测序(所谓的“下一代测序”)。

    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS
    10.
    发明申请
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIZING NUCLEIC ACIDS

    公开(公告)号:US20100120098A1

    公开(公告)日:2010-05-13

    申请号:US12605337

    申请日:2009-10-24

    摘要: The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.)

    摘要翻译: 本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法,组合物和试剂盒,然后使用DNA聚合酶产生5'-和3'标记 单链DNA片段,而不进行PCR扩增反应,其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列,3'-末端的第二个标签显示出 与第一个标签展示的序列不同的序列。 该方法可用于产生用于各种过程的5'-和3'标记的DNA片段,包括环境样品中DNA的宏基因组分析过程,DNA拷贝数变异(CNV)分析和比较基因组测序( CGS),包括大规模并行DNA测序(所谓的“下一代测序”)。