Ubiquitin E3 ligase
    7.
    发明授权
    Ubiquitin E3 ligase 有权
    泛素E3连接酶

    公开(公告)号:US07923219B2

    公开(公告)日:2011-04-12

    申请号:US11915610

    申请日:2006-06-01

    申请人: Hengbin Wang Yi Zhang

    发明人: Hengbin Wang Yi Zhang

    IPC分类号: C12Q1/37

    摘要: The present invention provides a native or reconstituted complex comprising Bmi1 and/or Ring1 and/or Ring2, wherein the complex has ubiquitin E3 ligase activity. Optionally, the complex further comprises HPH2 and/or HPC3. Also disclosed are methods of producing the reconstituted complex, methods of identifying compounds that modulate the ubiquitin E3 ligase activity of the native or reconstituted complex, and methods of identifying candidate compounds for treating cancer. Kits for determining modulation of protein ubiquitination and/or for ubiquitinating a target substrate are further provided.

    摘要翻译: 本发明提供了包含Bmi1和/或Ring1和/或Ring2的天然或重构的复合物,其中所述复合物具有泛素E3连接酶活性。 任选地,复合物还包含HPH 2和/或HPC 3。 还公开了产生重构复合物的方法,鉴定调节天然或重构复合物的泛素E3连接酶活性的化合物的方法以及鉴定用于治疗癌症的候选化合物的方法。 还提供了用于确定蛋白质泛素化和/或用于泛素化靶基质的调节的试剂盒。

    Purification, Characterization and Reconstitution of a Ubiquitin E3 Ligase
    9.
    发明申请
    Purification, Characterization and Reconstitution of a Ubiquitin E3 Ligase 有权
    泛素E3连接酶的纯化,表征和重构

    公开(公告)号:US20080248458A1

    公开(公告)日:2008-10-09

    申请号:US11915610

    申请日:2006-06-01

    摘要: The present invention provides a native or reconstituted complex comprising Bmi1 and/or Ring1 and/or Ring2, wherein the complex has ubiquitin E3 ligase activity. Optionally, the complex further comprises HPH2 and/or HPC3. Also disclosed are methods of producing the reconstituted complex, methods of identifying compounds that modulate the ubiquitin E3 ligase activity of the native or reconstituted complex, and methods of identifying candidate compounds for treating cancer. Kits for determining modulation of protein ubiquitination and/or for ubiquitinating a target substrate are further provided.

    摘要翻译: 本发明提供了包含Bmi1和/或Ring1和/或Ring2的天然或重构的复合物,其中所述复合物具有泛素E3连接酶活性。 任选地,复合物还包含HPH 2和/或HPC 3。 还公开了产生重构复合物的方法,鉴定调节天然或重构复合物的泛素E3连接酶活性的化合物的方法以及鉴定用于治疗癌症的候选化合物的方法。 还提供了用于确定蛋白质泛素化和/或用于泛素化靶基质的调节的试剂盒。