公开(公告)号：US09766256B2

公开(公告)日：2017-09-19

申请号：US15330443

申请日：2016-09-22

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/80

CPC分类号： G01N33/80 ,  G01N2446/00

摘要： Methods for separating, cells, particles, or other molecules of interest (MOI) from unwanted materials not of interest (MNOI). by forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, unwanted less dense materials from entering. Buoyant or other forces are used to remove any unbound material from the complexes with ASG before reading. Readable labels include the magnetic particle tagged complex itself, and other labels such as enzymes, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.

公开(公告)号：US09518984B2

公开(公告)日：2016-12-13

申请号：US13385427

申请日：2012-02-21

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/543 ,  G01N33/80

CPC分类号： G01N33/54326 ,  G01N33/80 ,  G01N2446/00

摘要： Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.

摘要翻译： 通过以新颖的方式观察潜在的抗原/抗体反应产物，在液体中检测感兴趣的物质，通常是红细胞上的抗体或抗原的方法。 反应产物沉积在磁性底物上，并且将表现出不同的性质，这取决于抗原/抗体反应是否发生。 抗原/抗体反应产物顽固地粘附到自身和底物上，同时任何未反应的磁性标记材料的沉积物弱粘附并容易地破坏并在不足以驱除反应的沉积物的破坏力下脱落。 内聚性的这种差异提供了一种积极结果与否定结果的区别。

公开(公告)号：US5571726A

公开(公告)日：1996-11-05

申请号：US446533

申请日：1995-05-19

申请人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

发明人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

IPC分类号： B01J13/00 ,  G01N33/543 ,  G01N33/558 ,  G01N33/58 ,  G01N33/74 ,  G01N33/551 ,  G01N33/553

CPC分类号： G01N33/587 ,  B01J13/0004 ,  B01J13/0043 ,  G01N33/54326 ,  G01N33/54386 ,  G01N33/558 ,  G01N33/74 ,  Y10S435/975

摘要： A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

摘要翻译： 用于各种免疫测定系统的方法和测试试剂盒。 提供一种制备含有预选尺寸的金属溶胶颗粒的金属溶胶试剂的方法。 在优化的pH条件下还原含金属溶液以制备预选尺寸的金属溶胶颗粒，用偶联化合物涂覆颗粒，然后与至少一种选择的免疫化学反应性组分结合。 具有不同免疫化学特异性的颗粒也混合以产生具有多种选择的免疫化学特异性的试剂。

公开(公告)号：US20160069877A1

公开(公告)日：2016-03-10

申请号：US14756975

申请日：2015-11-04

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/569

CPC分类号： G01N33/56966 ,  G01N33/5304 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54326 ,  G01N33/80 ,  G01N2333/705 ,  G01N2446/00

摘要： Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH).The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique.Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition.Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.

摘要翻译： 用于检测在具有Rh阴性细胞的混合群体中的至少两组细胞特别是Rh阳性细胞的混合细胞群中的一组细胞的存在或不存在并用于定量的方法，如在胎儿母体出血中发现的 （FMH）。 用抗D抗体包被的磁性颗粒与Rh阴性母体血液中的Rh阳性胎儿细胞反应，然后进行特异性分离和定量技术。 重力或磁力用于移动反应的磁性颗粒以分离，区分和定量由抗原组成分化的细胞。 Rh阳性细胞体积与原始血液样品的体积相关，作为需要施用于母亲以防止随后的Rh免疫的RhIG剂量数量的指示。

公开(公告)号：US4435293A

公开(公告)日：1984-03-06

申请号：US290267

申请日：1981-08-05

申请人： Henry A. Graham, Jr. ,  Johnna B. Hawk ,  Rosemary K. Chachowski

发明人： Henry A. Graham, Jr. ,  Johnna B. Hawk ,  Rosemary K. Chachowski

IPC分类号： G01N33/49 ,  B01D11/02 ,  B01L3/14 ,  B01L99/00 ,  B04B5/02 ,  B01D21/26 ,  B01L11/00

CPC分类号： B01L3/5021 ,  B01D11/02 ,  B01L99/00 ,  Y10T436/25375

摘要： A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, an air vent and, wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated within the inner tube but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.

摘要翻译： 描述了一种颗粒洗涤系统和使用方法，其中在优选的实施方案中，含有所需颗粒的流体被放置在其内部附近具有至少等于颗粒直径直径的孔的内管中， 并且其中所述内管定位在外管内，所述外管具有密度至少等于在所述内管内含有待分离的颗粒的溶液的密度至少等于或小于所述颗粒的密度的溶液的密度的流体。 向指向外管底部的颗粒施加离心力使颗粒移动通过孔口并通过外管中包含的外部溶液，使得颗粒从内部溶液中收集，由外部溶液洗涤， 随后沉积在外管的底部。

公开(公告)号：US09488665B2

公开(公告)日：2016-11-08

申请号：US11715411

申请日：2007-03-08

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/553 ,  G01N33/80

CPC分类号： G01N33/80 ,  G01N2446/00

摘要： Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering.Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading. Readable labels, in addition to the magnetic particle tagged complex itself, such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. In most embodiments, materials of interest are delivered to a special final zone for reading or harvesting. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.

摘要翻译： 特别涂覆有反应物的表面与标记的磁性颗粒复合体中的MOI反应，并且在读取之前使用浮力或其它力从表面去除任何未结合的材料。 可以使用除了磁性颗粒标记的复合体本身之外的可读标签，例如酶，荧光体，化学发光材料，放射性同位素，显色和荧光底物等标记。 在大多数实施例中，感兴趣的材料被输送到用于阅读或收获的特殊最后区域。 本发明适用于许多测定，诊断测试，分离程序和化学合成。

公开(公告)号：US20120214176A1

公开(公告)日：2012-08-23

申请号：US13385428

申请日：2012-02-21

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/566

CPC分类号： G01N33/56966 ,  G01N33/5304 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54326 ,  G01N33/80 ,  G01N2333/705 ,  G01N2446/00

摘要： Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH).The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique.Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition.Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.

摘要翻译： 用于检测在具有Rh阴性细胞的混合群体中的至少两组细胞特别是Rh阳性细胞的混合细胞群中的一组细胞的存在或不存在并用于定量的方法，如在胎儿母体出血中发现的 （FMH）。 用抗D抗体包被的磁性颗粒与Rh阴性母体血液中的Rh阳性胎儿细胞反应，然后进行特异性分离和定量技术。 重力或磁力用于移动反应的磁性颗粒以分离，区分和定量由抗原组成分化的细胞。 Rh阳性细胞体积与原始血液样品的体积相关，作为需要施用于母亲以防止随后的Rh免疫的RhIG剂量数量的指示。

公开(公告)号：US20120214175A1

公开(公告)日：2012-08-23

申请号：US13385427

申请日：2012-02-21

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/566

CPC分类号： G01N33/54326 ,  G01N33/80 ,  G01N2446/00

摘要： Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.

摘要翻译： 通过以新颖的方式观察潜在的抗原/抗体反应产物，在液体中检测感兴趣的物质，通常是红细胞上的抗体或抗原的方法。 反应产物沉积在磁性底物上，并且将表现出不同的性质，这取决于抗原/抗体反应是否发生。 抗原/抗体反应产物顽固地粘附到自身和底物上，同时任何未反应的磁性标记材料的沉积物弱粘附并容易地破坏并在不足以驱除反应的沉积物的破坏力下脱落。 内聚性的这种差异提供了一种积极结果与否定结果的区别。

公开(公告)号：US10107811B2

公开(公告)日：2018-10-23

申请号：US14756975

申请日：2015-11-04

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/80 ,  G01N33/569 ,  G01N33/541 ,  G01N33/537 ,  G01N33/53 ,  G01N33/543

摘要： Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH).The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique.Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition.Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.

公开(公告)号：US20170016922A1

公开(公告)日：2017-01-19

申请号：US15330443

申请日：2016-09-22

申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

IPC分类号： G01N33/80

CPC分类号： G01N33/80 ,  G01N2446/00

摘要： Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering.Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading. Readable labels, in addition to the magnetic particle tagged complex itself, such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. In most embodiments, materials of interest are delivered to a special final zone for reading or harvesting. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.

摘要翻译： 特别涂覆有反应物的表面与标记的磁性颗粒复合体中的MOI反应，并且在读取之前使用浮力或其它力从表面去除任何未结合的材料。 可以使用除了磁性颗粒标记的复合体本身之外的可读标签，例如酶，荧光体，化学发光材料，放射性同位素，显色和荧光底物等标记。 在大多数实施例中，感兴趣的材料被输送到用于阅读或收获的特殊最后区域。 本发明适用于许多测定，诊断测试，分离程序和化学合成。