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公开(公告)号：US20090233332A1
公开(公告)日：2009-09-17
申请号：US12090022
申请日：2006-09-26
申请人： Hiroshi Shoji , Toshikazu Sugimoto
发明人： Hiroshi Shoji , Toshikazu Sugimoto
IPC分类号： C12P21/06
摘要： An object of the present invention is to provide a method of mass-producing recombinant protein by the liquid culture method with koji molds as host. According to the present invention, it is provided a method of producing recombinant protein by using the recombinant koji molds which are obtained by transforming koji molds as host comprising: culturing the recombinant koji molds in a liquid medium which contains as culture raw material at least one selected from the group consisting of the cereal of which surface is entirely or partly covered with at least husks, the bean and/or the tuber of which surface is covered with hulls and the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing; and collecting the recombinant protein from the culture product.
摘要翻译： 本发明的目的是提供一种通过液体培养法以曲霉为主体大量生产重组蛋白的方法。 根据本发明，提供了通过使用通过转化曲霉作为宿主获得的重组曲霉菌来制备重组蛋白的方法，包括：将重组曲霉菌在含有作为培养原料的液体培养基中培养至少一种 选自由表面完全或部分地被至少外壳覆盖的谷物组成的组，其中表面被壳体覆盖的豆和/或块茎，苋菜和/或藜麦没有预处理如研磨 或破碎 并从培养产物中收集重组蛋白。
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公开(公告)号：US08802170B2
公开(公告)日：2014-08-12
申请号：US11547809
申请日：2005-03-24
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： A23L1/20
摘要： An object of the present invention is to provide a liquid koji to be used in the production of a fermented food or drink, particularly a liquid koji having glucoamylase and acid-stable α-amylase with high enzymatic activities, which can be used in the brewing of shochu. The present invention provides a method of manufacturing a liquid koji to be used in production of a fermented food or drink, comprising: cultivating a koji mold in a liquid medium that contains, as a raw material, a substance selected from the group consisting of a cereal having a surface covered with a husk; a cereal having a surface from which only a husk (e.g., a chaff) is removed; an unprocessed bean or tuber having a surface covered with a hull; and amaranthus and/or quinoa. According to the present invention, both enzymes of glucoamylase and acid-stable α-amylase are simultaneously produced with high yield in a balanced manner to allow the production of a liquid koji having enzymatic activities required for, for example, the brewing of shochu. By using the liquid koji, fermented foods and drinks such as shochu can be efficiently produced.
摘要翻译： 本发明的目的是提供一种用于生产发酵食品或饮料的液体曲，特别是具有高酶活性的葡糖淀粉酶和酸稳定性α-淀粉酶的液体曲，可用于酿造 的烧酒。 本发明提供一种制造用于生产发酵食品或饮料的液体曲的方法，包括：在液体培养基中培养曲霉，所述液体培养基含有选自以下的物质作为原料： 谷物表面覆盖着果壳; 具有仅除去壳（例如谷壳）的表面的谷物; 未加工的豆或块茎，其表面覆盖有船体; 和苋菜和/或藜麦。 根据本发明，以均衡的方式同时产生葡糖淀粉酶和酸稳定性α-淀粉酶的两种酶，以制备具有例如烧制啤酒所需的酶活性的液体曲。 通过使用液体曲，可以有效地生产发酵食品和饮料如烧酒。
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3.发明授权公开(公告)号：US06911563B2
公开(公告)日：2005-06-28
申请号：US10451624
申请日：2001-12-27
申请人： Fujio Mizukami , Shuichi Niwa , Makoto Toba , Naotsugu Itoh , Tomonari Saito , Takemi Nanba , Hiroshi Shoji , Kazuhiko Haba
发明人： Fujio Mizukami , Shuichi Niwa , Makoto Toba , Naotsugu Itoh , Tomonari Saito , Takemi Nanba , Hiroshi Shoji , Kazuhiko Haba
IPC分类号： B01J8/00 , B01J10/00 , B01J12/00 , B01J14/00 , B01J19/24 , B01J23/44 , B01J35/06 , C07C29/50 , C07C37/58 , C07C37/60 , C07C39/04 , C07C39/07 , C07C45/33 , C07C45/34 , C07C45/35 , C07C45/36 , C07C45/40 , C07D213/63 , C07L37/00
CPC分类号： C07D213/63 , B01J8/009 , B01J10/007 , B01J12/007 , B01J14/005 , B01J19/2475 , B01J23/44 , B01J35/065 , B01J2219/00063 , B01J2219/00094 , B01J2219/00099 , C07C29/50 , C07C37/58 , C07C37/60 , C07C45/33 , C07C45/34 , C07C45/35 , C07C45/36 , C07C45/40 , C07C2601/14 , Y02P20/52 , C07C49/08 , C07C47/22 , C07C35/08 , C07C39/04 , C07C39/07 , C07C39/14 , C07C35/36
摘要： A method for carrying out a reaction of one substance capable of being activated by a catalyst with another substance capable of reacting with said one substance activated, characterized in that the substance capable of being activated is activated by passing the substance through a diaphragm type catalyst and the reaction is thus performed in one reaction step; a method for producing an aromatic alcohol utilizing the above method; and a reaction apparatus suitable for these reactions. In the method, one substance is activated by passing through a diaphragm type catalyst and an objective reaction is carried out by using the activated substance, and the reaction can be performed in one reaction step and with safety. Moreover, the contact of the above activated substance with a compound to be reacted therewith can be freely controlled, and therefore, over-reaction can be prevented and an objective product can be produced in high yield. The method is thus markedly advantageous from an economical view point as a commercial process for producing oxygen-containing organic compounds such as an aromatic alcohol, a ketone, an aldehyde, a carboxylic acid and an epoxide.
摘要翻译： 一种能够使一种能够被催化剂活化的物质与能够与所述一种活化物质反应的物质进行反应的方法，其特征在于，通过使该物质通过隔膜型催化剂而活化， 因此在一个反应步骤中进行反应; 利用上述方法制备芳族醇的方法; 和适用于这些反应的反应装置。 在该方法中，通过隔膜型催化剂活化一种物质，并通过使用活性物质进行目标反应，反应可以在一个反应步骤和安全性下进行。 此外，可以自由地控制上述活性物质与其反应的化合物的接触，因此可以防止过度反应，并且可以高产率制备目标产物。 因此，作为用于生产含氧有机化合物如芳族醇，酮，醛，羧酸和环氧化物的商业方法，从经济角度看，该方法显着是有利的。
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公开(公告)号：US20100120119A1
公开(公告)日：2010-05-13
申请号：US11995942
申请日：2006-07-20
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： C12N9/30
摘要： An object of the present invention is to provide a method of producing liquid koji having high enzymatic activity by optimizing composition of liquid medium for the liquid koji. In particular, the present invention provides a method of producing liquid koji having enhanced enzymatic activity, the method comprising culturing white koji molds and/or black koji molds in liquid medium containing a nitrogen source by using the cereal of which surface is entirely or partly covered with husks as culture raw material.
摘要翻译： 本发明的目的是提供通过优化用于液体曲的液体介质的组成来生产具有高酶活性的液体曲的方法。 特别地，本发明提供了具有增强的酶活性的液体曲的生产方法，该方法包括通过使用表面完全或部分覆盖的谷物来培养含有氮源的液体培养基中的白曲霉和/或黑曲霉 以壳为文化原料。
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公开(公告)号：US20070207238A1
公开(公告)日：2007-09-06
申请号：US11547809
申请日：2005-03-24
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： A23L1/20
摘要： An object of the present invention is to provide a liquid koji to be used in the production of a fermented food or drink, particularly a liquid koji having glucoamylase and acid-stable α-amylase with high enzymatic activities, which can be used in the brewing of shochu. The present invention provides a method of manufacturing a liquid koji to be used in production of a fermented food or drink, comprising: cultivating a koji mold in a liquid medium that contains, as a raw material, a substance selected from the group consisting of a cereal having a surface covered with a husk; a cereal having a surface from which only a husk (e.g., a chaff) is removed; an unprocessed bean or tuber having a surface covered with a hull; and amaranthus and/or quinoa. According to the present invention, both enzymes of glucoamylase and acid-stable α-amylase are simultaneously produced with high yield in a balanced manner to allow the production of a liquid koji having enzymatic activities required for, for example, the brewing of shochu. By using the liquid koji, fermented foods and drinks such as shochu can be efficiently produced.
摘要翻译： 本发明的目的是提供一种用于生产发酵食品或饮料的液体曲，特别是具有高酶活性的具有葡糖淀粉酶和酸稳定的α-淀粉酶的液体曲，可用于酿造中 的烧酒。 本发明提供一种制造用于生产发酵食品或饮料的液体曲的方法，包括：在液体培养基中培养曲霉，所述液体培养基含有选自以下的物质作为原料： 谷物表面覆盖着果壳; 具有仅除去壳（例如谷壳）的表面的谷物; 未加工的豆或块茎，其表面覆盖有船体; 和苋菜和/或藜麦。 根据本发明，以均衡的方式同时以高产率生产葡糖淀粉酶和酸稳定性α-淀粉酶的两种酶，以制备具有例如烧制啤酒所需的酶活性的液体曲。 通过使用液体曲，可以有效地生产发酵食品和饮料如烧酒。
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公开(公告)号：US08124374B2
公开(公告)日：2012-02-28
申请号：US12090022
申请日：2006-09-26
申请人： Hiroshi Shoji , Toshikazu Sugimoto
发明人： Hiroshi Shoji , Toshikazu Sugimoto
摘要： An object of the present invention is to provide a method of mass-producing recombinant protein by the liquid culture method with koji molds as host. According to the present invention, it is provided a method of producing recombinant protein by using the recombinant koji molds which are obtained by transforming koji molds as host comprising: culturing the recombinant koji molds in a liquid medium which contains as culture raw material at least one selected from the group consisting of the cereal of which surface is entirely or partly covered with at least husks, the bean and/or the tuber of which surface is covered with hulls and the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing; and collecting the recombinant protein from the culture product.
摘要翻译： 本发明的目的是提供一种通过液体培养法以曲霉为主体大量生产重组蛋白的方法。 根据本发明，提供了通过使用通过转化曲霉作为宿主获得的重组曲霉菌来制备重组蛋白的方法，包括：将重组曲霉菌在含有作为培养原料的液体培养基中培养至少一种 选自由表面完全或部分地被至少外壳覆盖的谷物组成的组，其中表面被壳体覆盖的豆和/或块茎，苋菜和/或藜麦没有预处理如研磨 或破碎 并从培养产物中收集重组蛋白。
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公开(公告)号：US20110220026A1
公开(公告)日：2011-09-15
申请号：US13129924
申请日：2009-11-19
申请人： Eiji Ino , Akira Watanabe , Shunichi Ishihara , Hajime Ashida , Hiroshi Shoji , Keiichi Yoshimura
发明人： Eiji Ino , Akira Watanabe , Shunichi Ishihara , Hajime Ashida , Hiroshi Shoji , Keiichi Yoshimura
IPC分类号： C23C16/505 , C23C16/455 , C23C16/458
CPC分类号： H01J37/32568 , C23C16/45578 , C23C16/509 , H01J37/3244
摘要： A plasma processing device according to the present invention includes a plasma processing chamber consisting of a vacuum container, a pair of substrate holders standing opposite each other in the plasma processing chamber, a plurality of first reaction gas tubes provided between the two substrate holders, and a plurality of second reaction gas tubes provided between the plurality of first reaction gas tubes and each of the two substrate holders. The first reaction gas tubes, which are made of an electrically conductive material, are electrically connected to a first radio-frequency power source or second radio-frequency power source. The first reaction gas tubes double as a radio-frequency antenna, while the second reaction gas tubes double as an electrode.
摘要翻译： 根据本发明的等离子体处理装置包括等离子体处理室，其由真空容器，在等离子体处理室中彼此相对定位的一对基板保持器组成，多个第一反应气体管设置在两个基板保持器之间，以及 设置在所述多个第一反应气体管与所述两个基板保持件中的每一个之间的多个第二反应气体管。 由导电材料制成的第一反应气体管与第一射频电源或第二射频电源电连接。 第一反应气管作为射频天线加倍，而第二反应气管作为电极加倍。
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8.发明授权Method of producing liquid koji having enhanced plant fiber degeneration enzyme, liquid koji obtained by the method and use thereof 有权具有增强的植物纤维变性酶的液体曲的制备方法及其使用获得的液体曲公开(公告)号：US07998715B2
公开(公告)日：2011-08-16
申请号：US12067423
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
摘要： An object of the present invention is to provide a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme using liquid medium without using an expensive plant fiber degradation enzyme preparation and a recombinant bacterium and methods of producing liquid koji dry product and industrial alcohol (ethanol) using the liquid koji. According to the present invention, there is provided a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme by culturing koji molds with at least one liquid medium selected from the group consisting of a liquid medium containing as culture raw material the cereal of which surface is entirely or partly covered with at least husks, a liquid medium containing as culture raw material the bean and/or the tuber of which surface is covered with hulls, and a liquid medium containing as culture raw material the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing, wherein amount of the culture raw material to be used in the liquid medium is controlled to generate and accumulate simultaneously at least glucoamylase, acid-stable α-amylase and a plant fiber degradation enzyme in the koji mold culture product. There are also provided a method of producing a dry product of liquid koji with liquid medium, characterized by drying the liquid koji obtained by the above-mentioned method, and a method of producing ethanol by a fermentation method using the liquid koji.
摘要翻译： 本发明的目的是提供一种使用液体培养基而不使用昂贵的植物纤维降解酶制剂和重组细菌的植物纤维降解酶的活性增加的液体曲的制造方法和制造液体曲柄干燥产品和工业的方法 酒精（乙醇）使用液体曲。 根据本发明，提供了一种制备具有增强植物纤维降解酶活性的液体曲的方法，该方法通过用至少一种液体培养基培养曲霉，所述液体培养基选自含有作为培养原料的谷物的液体培养基 其表面完全或部分地被至少覆盖壳，包含作为培养原料的豆类和/或其表面被壳体覆盖的块茎的液体培养基，以及含有作为培养原料的苋菜和/或 不经预处理的藜麦如研磨或粉碎，其中控制在液体介质中使用的培养原料的量至少同时产生并积累至少葡糖淀粉酶，酸稳定的α-淀粉酶和植物纤维降解酶 曲曲霉菌产品。 还提供了一种用液体培养基制备液体曲的干燥产物的方法，其特征在于干燥通过上述方法获得的液体曲，以及通过使用液体曲的发酵方法生产乙醇的方法。
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9.发明申请METHOD OF PRODUCING LIQUID KOJI HAVING ENHANCED PLANT FIBER DEGRADATION ENZYME, LIQUID KOJI OBTAINED BY THE METHOD AND USE THEREOF 有权生产具有增强植物纤维降解酶的液体醇的方法和方法获得的液体KOJO公开(公告)号：US20090022848A1
公开(公告)日：2009-01-22
申请号：US12067423
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
摘要： An object of the present invention is to provide a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme using liquid medium without using an expensive plant fiber degradation enzyme preparation and a recombinant bacterium and methods of producing liquid koji dry product and industrial alcohol (ethanol) using the liquid koji. According to the present invention, there is provided a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme by culturing koji molds with at least one liquid medium selected from the group consisting of a liquid medium containing as culture raw material the cereal of which surface is entirely or partly covered with at least husks, a liquid medium containing as culture raw material the bean and/or the tuber of which surface is covered with hulls, and a liquid medium containing as culture raw material the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing, wherein amount of the culture raw material to be used in the liquid medium is controlled to generate and accumulate simultaneously at least glucoamylase, acid-stable α-amylase and a plant fiber degradation enzyme in the koji mold culture product. There are also provided a method of producing a dry product of liquid koji with liquid medium, characterized by drying the liquid koji obtained by the above-mentioned method, and a method of producing ethanol by a fermentation method using the liquid koji.
摘要翻译： 本发明的目的是提供一种使用液体培养基而不使用昂贵的植物纤维降解酶制剂和重组细菌的植物纤维降解酶的活性增加的液体曲的制造方法和制造液体曲柄干燥产品和工业的方法 酒精（乙醇）使用液体曲。 根据本发明，提供了一种制备具有增强植物纤维降解酶活性的液体曲的方法，该方法通过用至少一种液体培养基培养曲霉，所述液体培养基选自含有作为培养原料的谷物的液体培养基 其表面完全或部分地被至少覆盖壳，包含作为培养原料的豆类和/或其表面被壳体覆盖的块茎的液体培养基，以及含有作为培养原料的苋菜和/或 没有预处理的藜麦如研磨或粉碎，其中控制在液体介质中使用的培养原料的量至少同时产生并积累至少葡糖淀粉酶，酸稳定的α-淀粉酶和植物纤维降解酶 曲曲霉菌产品。 还提供了一种用液体培养基制备液体曲的干燥产物的方法，其特征在于干燥通过上述方法获得的液体曲，以及通过使用液体曲的发酵方法生产乙醇的方法。
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公开(公告)号：US4406193A
公开(公告)日：1983-09-27
申请号：US292302
申请日：1981-08-12
申请人： Yoshihide Sugino , Hiroshi Shoji
发明人： Yoshihide Sugino , Hiroshi Shoji
CPC分类号： B23B31/00 , B25B5/062 , Y10T82/2514 , Y10T82/2572
摘要： A workpiece is urged against a chuck by a press plate supported by one end of a piston rod through a ball bearing. A rear end of a cylinder for the piston rod is swingably supported by a ball interposed between the rear surface of the cylinder and a stationary plate. Bolts for securing the cylinder to the stationary plate are formed with tapered portions mating with the tapered openings of bushings fitted to the supporting plate so as to selectively permit swinging motion of the cylinder.
摘要翻译： 通过由活塞杆的一端支撑的压板通过滚珠轴承将工件推靠在卡盘上。 用于活塞杆的气缸的后端由插入在气缸的后表面和固定板之间的球可摆动地支撑。 用于将气缸固定到固定板上的螺栓形成有与装配到支撑板上的衬套的锥形开口配合的锥形部分，以便选择性地允许气缸的摆动。
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