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    Apparatus and methods for chemical synthesis
    1.
    发明授权
    Apparatus and methods for chemical synthesis 失效
    用于化学合成的装置和方法

    公开(公告)号：US06867050B2

    公开(公告)日：2005-03-15

    申请号：US09923464

    申请日：2001-08-06

    申请人： Konan Peck ,  Ji-Yen Cheng

    发明人： Konan Peck ,  Ji-Yen Cheng

    IPC分类号： B01J19/00 ,  G01N1/10 ,  B01L3/02 ,  G01N15/06 ,  G01N21/00

    CPC分类号： B01J19/0046 ,  B01J2219/00274 ,  Y10T436/2575

    摘要： Apparatuses and methods are described for parallel oligonucleotide synthesis of hundreds of different sequences and lengths at a time. Standard phosphoramidite chemistry is employed. The syntheses take place in a reaction plate compatible with the industrial standard microplate format to allow the use of readily available automated instruments for subsequent processing. Key parameters in reducing synthesis volume in small reaction wells are discussed. This invention provides solutions to the difficulties of low volume, high number synthetic reactions.

    摘要翻译： 描述了一次并行寡核苷酸合成数百种不同序列和长度的装置和方法。 使用标准亚磷酰胺化学。 合成在与工业标准微板格式兼容的反应板中进行，以允许使用容易获得的自动化仪器进行后续处理。 讨论了在小反应井中降低合成体积的关键参数。 本发明提供了低容量，高数量的合成反应的困难的解决方案。

    High sensitivity fluorescent single particle and single molecule detection apparatus and method
    2.
    发明授权
    High sensitivity fluorescent single particle and single molecule detection apparatus and method 失效
    高灵敏度荧光单粒子和单分子检测仪器及方法

    公开(公告)号：US4979824A

    公开(公告)日：1990-12-25

    申请号：US358782

    申请日：1989-05-26

    申请人： Richard A. Mathies ,  Konan Peck ,  Lubert Stryer

    发明人： Richard A. Mathies ,  Konan Peck ,  Lubert Stryer

    IPC分类号： G01N21/63 ,  G01N15/02 ,  G01N15/14 ,  G01N21/64 ,  G01N33/543

    CPC分类号： G01N21/645 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1475 ,  G01N2015/0222 ,  G01N21/6456

    摘要： Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.

    摘要翻译： 描述了用于在流体或基板上的单个荧光分子极限处的超灵敏检测的装置，其包括用于照射预定体积的基板的流体或区域的装置，从而发射包括来自流体的背景光的光，以及 从位于该地区的颗粒中发射光子。 实时检测光子脉冲以产生输出代表信号。 信号被接收，并且来自荧光颗粒的能量爆发与背景能区别开来，以提供颗粒或分子的数量，位置或浓度的指示。

    Liquid chromatography absorbance detector
    5.
    发明授权
    Liquid chromatography absorbance detector 失效
    液相色谱吸收检测器

    公开(公告)号：US4795262A

    公开(公告)日：1989-01-03

    申请号：US79026

    申请日：1987-07-29

    申请人： Michael D. Morris ,  Teng-Ke J. Pang ,  Konan Peck

    发明人： Michael D. Morris ,  Teng-Ke J. Pang ,  Konan Peck

    IPC分类号： G01N21/03 ,  G01N21/25 ,  G01N21/85 ,  G01N30/74 ,  G02B5/12 ,  G01N21/01

    CPC分类号： G01N21/255 ,  G01N30/74 ,  G01N21/031 ,  G01N21/85 ,  G02B5/12

    摘要： This invention relates to an absorbance detector particularly adapted to liquid chromatography testing. In modern chromatography techniques, light is focused within a small capacity test sample cell, and an optical system is provided to measure the absorbance of various frequencies of light to thereby identify the existence of selected compounds. Inaccuracies of presently available detecting devices result since localized heating within the test cell generates localized index of refraction variations which refract the light in a random fashion such that it is not sensed by the detector. Additional distorting effects are caused by imperfections in the optical surfaces of the system and turbulence due to fluid flow within the sample cell. In accordance with this invention, an appropriate phase conjugator in the form of a retroreflective array is placed in the path of light exiting the sample cell. The array redirects the light rays along their original paths such that they are again distorted but in a reverse sense from the original distortion. In this manner, all of the distorting effects are compensated for. In addition, various means are employed to eliminate the effects of specular reflection from the various optical elements within the system.

    摘要翻译： 本发明涉及一种特别适用于液相色谱测试的吸光度检测器。 在现代色谱技术中，光聚焦在小容量测试样品池中，并且提供光学系统以测量各种频率的光的吸光度，从而鉴定所选化合物的存在。 目前可用的检测装置的不精确性是由于测试单元内的局部加热产生局部折射率折射率，其以随机方式折射光，使得其不被检测器感测。 额外的扭曲效应是由于系统的光学表面的缺陷和由于样品池内的流体流动引起的湍流引起的。 根据本发明，将回射阵列形式的合适的相位共轭器放置在离开样品池的光的路径中。 阵列将光线沿其原始路径重定向，使得它们再次失真，但是与原始失真相反。 以这种方式，所有的扭曲效应得到补偿。 此外，采用各种方法来消除系统内各种光学元件的镜面反射的影响。

    RAPID EFFICACY ASSESSMENT METHOD FOR LUNG CANCER THERAPY
    6.
    发明申请
    RAPID EFFICACY ASSESSMENT METHOD FOR LUNG CANCER THERAPY 审中-公开
    用于肺癌治疗的快速有效评估方法

    公开(公告)号：US20090220985A1

    公开(公告)日：2009-09-03

    申请号：US12410040

    申请日：2009-03-24

    申请人： Konan Peck ,  Yuh-Pyng Sher ,  Jin-Yuan Shih ,  Pan-Chyr Yang ,  Cheng-Wen Wu

    发明人： Konan Peck ,  Yuh-Pyng Sher ,  Jin-Yuan Shih ,  Pan-Chyr Yang ,  Cheng-Wen Wu

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118

    摘要： The present invention discloses a method for rapid assessment of lung cancer therapy efficacy in a few days instead of weeks by conventional imaging methods. This method can also be used to detect relapse of the cancer and to improve the current TNM cancer staging method for more accurate prognosis. The rapid assessment of therapy efficacy is based on detecting circulating cancer cells in body fluid with high positive detection rate. The high positive detection rate is achieved by using qPCR amplification of multiple marker genes identified by in silico search of DNA sequence database. This invention also discloses a scoring method to calculate the cancer cell load based on qPCR results to correlate the amount of circulating cancer cells in lung cancer patients and predict the treatment outcomes.

    摘要翻译： 本发明公开了一种通过常规成像方法在几天而不是几周内快速评估肺癌疗效的方法。 该方法也可用于检测癌症的复发，并改善目前的TNM癌症分期方法，以获得更准确的预后。 治疗效果的快速评估是基于以高阳性检出率检测体液中的循环癌细胞。 通过使用通过DNA序列数据库的计算机检索鉴定的多个标记基因的qPCR扩增来实现高阳性检测率。 本发明还公开了一种基于qPCR结果计算癌细胞负荷的评分方法，以将肺癌患者中循环癌细胞的量相关并预测治疗结果。

    Rapid efficacy assessment method for lung cancer therapy
    8.
    发明授权
    Rapid efficacy assessment method for lung cancer therapy 有权
    肺癌治疗快速疗效评估方法

    公开(公告)号：US07507534B2

    公开(公告)日：2009-03-24

    申请号：US11306532

    申请日：2005-12-31

    申请人： Konan Peck ,  Yuh-Pyng Sher ,  Jin-Yuan Shih ,  Pan-Chyr Yang ,  Cheng-Wen Wu

    发明人： Konan Peck ,  Yuh-Pyng Sher ,  Jin-Yuan Shih ,  Pan-Chyr Yang ,  Cheng-Wen Wu

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118

    摘要： The present invention discloses a method for rapid assessment of lung cancer therapy efficacy in a few days instead of weeks by conventional imaging methods. This method can also be used to detect relapse of the cancer and to improve the current TNM cancer staging method for more accurate prognosis. The rapid assessment of therapy efficacy is based on detecting circulating cancer cells in body fluid with high positive detection rate. The high positive detection rate is achieved by using qPCR amplification of multiple marker genes identified by in silico search of DNA sequence database. This invention also discloses a scoring method to calculate the cancer cell load based on qPCR results to correlate the amount of circulating cancer cells in lung cancer patients and predict the treatment outcomes.

    摘要翻译： 本发明公开了一种通过常规成像方法在几天而不是几周内快速评估肺癌疗效的方法。 该方法也可用于检测癌症的复发，并改善目前的TNM癌症分期方法，以获得更准确的预后。 治疗效果的快速评估是基于以高阳性检出率检测体液中的循环癌细胞。 通过使用通过DNA序列数据库的计算机检索鉴定的多个标记基因的qPCR扩增来实现高阳性检测率。 本发明还公开了一种基于qPCR结果计算癌细胞负荷的评分方法，以将肺癌患者中循环癌细胞的量相关并预测治疗结果。

    Photothermal densitometer for reading electrophoresis gels
    9.
    发明授权
    Photothermal densitometer for reading electrophoresis gels 失效
    用于读取电泳凝胶的光热密度计

    公开(公告)号：US4938593A

    公开(公告)日：1990-07-03

    申请号：US226627

    申请日：1988-08-10

    申请人： Michael D. Morris ,  Konan Peck

    发明人： Michael D. Morris ,  Konan Peck

    IPC分类号： G01N21/17 ,  G01N21/59 ,  G01N27/447

    CPC分类号： G01N21/171 ,  G01N27/44721 ,  G01N21/5911 ,  G01N2201/1087

    摘要： A densitometer apparatus for evaluating electrophoresis gel samples based on photothermal techniques. In accordance with this invention, electrophoresis gels are characterized by passing a heating light beam through the gel at a particular location. Light absorbed by the presence of staining dyes in that area causes heat evolution which generates a local index of refraction variation or a "thermal lens". A probe beam is passed through the sample in the area of the thermal lens a predetermined period of time after it is generated and the modification to the beam caused by the thermal lens is evaluated. For example, defocusing of the probe beam can be sensed by a detector which receives transmitted light through a limiting aperture. Various means of separating the heating and probe beams are disclosed, including use of separate lasers, crossed beams, modulation by plane of polarization, etc. One embodiment of this invention is particularly adapted for characterizing dry gels in which the heating beam is absorbed by the sample and the probe beam passes across the sample and is modified by a thermal lens generated in the air above the sample. Several embodiments are related to means for offsetting the probe beam from the heating beam for use with samples that are swept by the photothermal techniques in accordance with this invention offer advantages in terms of sensitivity over conventional transmission-type densitometers. These advantages enable increased sensitivity and facilitate the use of simplified staining techniques.

    摘要翻译： 一种用于基于光热技术评估电泳凝胶样品的浓度计装置。 根据本发明，电泳凝胶的特征在于使加热光束在特定位置通过凝胶。 由于该区域染色染料的存在而吸收的光导致产生局部折射率变化或“透镜”的散热。 探测光束在产生之后的预定时间段内通过热透镜的区域中的样品，并且评估由热透镜引起的光束的修改。 例如，探测光束的散焦可以由接收透射光通过限制孔径的检测器来感测。 公开了分离加热和探针光束的各种装置，包括使用单独的激光器，交叉光束，通过偏振平面的调制等。本发明的一个实施例特别适用于表征干凝胶，其中加热光束被吸收 样品和探针光束穿过样品，并通过样品上方的空气中产生的热透镜进行修饰。 几个实施例涉及用于将探针光束从加热光束偏移的装置，以便与根据本发明的光热技术扫描的样品一起使用，以提供与传统透射型密度计相比的灵敏度方面的优点。 这些优点使得灵敏度增加并且便于使用简化的染色技术。