公开(公告)号：US07393640B2

公开(公告)日：2008-07-01

申请号：US10772996

申请日：2004-02-05

申请人： Shiv Kumar ,  Mark McDougall ,  Anup Sood ,  John Nelson ,  Carl Fuller ,  John Macklin ,  Paul Mitsis

发明人： Shiv Kumar ,  Mark McDougall ,  Anup Sood ,  John Nelson ,  Carl Fuller ,  John Macklin ,  Paul Mitsis

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12P19/34

摘要： The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

摘要翻译： 本发明描述了在锰盐存在下使用末端磷酸酯标记的核苷酸以增强其对各种酶的底物性质的方法。 特别描述的是在锰盐存在的情况下，基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物，检测样品中的核酸的方法。 还提供了末端磷酸酯标记的核苷酸的锰络合物以及具有增强的底物性质的新接头的末端磷酸酯标记的核苷酸。

公开(公告)号：US20060013518A1

公开(公告)日：2006-01-19

申请号：US11178937

申请日：2005-07-11

申请人： Robert Foster ,  Paul Bien ,  John Macklin

发明人： Robert Foster ,  Paul Bien ,  John Macklin

IPC分类号： F16C33/02 ,  F16C33/24 ,  F16C1/00 ,  F16C1/24 ,  F16C3/14 ,  F16C5/00 ,  F16C33/10 ,  F16C33/16

CPC分类号： F16C33/103 ,  F16C33/1065

摘要： An improved sleeve bearing for locomotive traction motor that provides additional lubrication over and above that of the one central wick currently used. The sleeve bearing is provided with a recirculation system comprised of either at least two additional wicks located near the 6:00 operating location or multiple oil communication and delivery grooves passageways communicating with the oil flinger grooves placed for the purpose of collecting and providing a mechanism for providing additional lubrication for extending the oil-wetted envelope available for supporting the truck-axle journal in the axial direction.

公开(公告)号：US07560254B2

公开(公告)日：2009-07-14

申请号：US10651558

申请日：2003-08-29

申请人： Anup Sood ,  Shiv Kumar ,  Carl Fuller ,  John Nelson ,  John Macklin

发明人： Anup Sood ,  Shiv Kumar ,  Carl Fuller ,  John Nelson ,  John Macklin

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/125 ,  C12Q2521/525 ,  C12Q2521/319

摘要： A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3′→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.

摘要翻译： 提供了表征核酸样品的方法，其包括以下步骤：（a）进行DNA聚合酶反应，其包括模板，等位基因特异性引物，至少一个末端磷酸酯标记的核苷酸，DNA聚合酶和 任选地当引物不可水解时具有3'→5'核酸外切酶活性的酶，该反应导致标记的多磷酸盐的产生; （b）允许标记的多磷酸酯与磷酸酶反应以产生可检测的物质; （c）检测可检测物种; 和（d）基于这种检测来表征核酸样品。

公开(公告)号：US20070165974A1

公开(公告)日：2007-07-19

申请号：US11680729

申请日：2007-03-01

申请人： Robert Foster ,  Paul Bien ,  John Macklin

发明人： Robert Foster ,  Paul Bien ,  John Macklin

IPC分类号： F16C33/10

CPC分类号： F16C33/103 ,  B61C9/38 ,  B61C17/08 ,  F16C17/02 ,  F16C33/106 ,  F16C33/1065 ,  F16C33/18 ,  F16C33/24

摘要： An improved sleeve bearing for locomotive traction motor that provides additional lubrication over and above that of the one central wick currently used. The sleeve bearing is provided with a recirculation system comprised of either at least two additional wicks located near the 6:00 operating location or multiple oil communication and delivery grooves passageways communicating with the oil flinger grooves placed for the purpose of collecting and providing a mechanism for providing additional lubrication for extending the oil-wetted envelope available for supporting the truck-axle journal in the axial direction.

摘要翻译： 一种用于机车牵引电动机的改进的套筒轴承，其提供超过当前使用的一个中心芯的额外润滑。 套筒轴承设置有再循环系统，该再循环系统由位于6:00操作位置附近的至少两个额外的芯组成，或者与设置用于​​收集和提供机构的油浸沟槽连通的多个油连通和输送槽通道 提供额外的润滑，以延伸油润滑的信封，用于在轴向方向上支撑卡车轴颈。

公开(公告)号：US07189476B1

公开(公告)日：2007-03-13

申请号：US10069011

申请日：2000-08-18

申请人： William John Macklin ,  Derek John Fray

发明人： William John Macklin ,  Derek John Fray

IPC分类号： H01M4/58 ,  H01M4/60

CPC分类号： H01M4/583 ,  B82Y30/00 ,  H01M4/133 ,  H01M10/052

摘要： An anode for a rechargeable lithium cell comprises carbon nanotubes that contain within them an element that can form alloys or compounds reversibly with lithium over a range of compositions. The element within the nanotubes may be aluminum or tin. These carbon nanotubes are bound together to form a coherent layer with a polymeric binder, such as a polymer of viniylidene fluoride. A cell with such an anode should have improved capacity and improved reversibility, because the nanotubes provide a stabilizing framework for the alloy.

摘要翻译： 用于可再充电锂电池的阳极包括碳纳米管，其中包含可在一定范围的组合物中与锂可逆地形成合金或化合物的元素。 纳米管内的元素可以是铝或锡。 这些碳纳米管被结合在一起以形成具有聚合物粘合剂的粘合层，例如维非二氟乙烯的聚合物。 具有这种阳极的电池应该具有改进的容量和改进的可逆性，因为纳米管为合金提供稳定的框架。

公开(公告)号：US20050022476A1

公开(公告)日：2005-02-03

申请号：US10632611

申请日：2003-08-01

申请人： Craig Hamer ,  John Macklin ,  Gary Schukar ,  Jeffery Jackson ,  Neil Schwartzbauer

发明人： Craig Hamer ,  John Macklin ,  Gary Schukar ,  Jeffery Jackson ,  Neil Schwartzbauer

IPC分类号： B29C65/18 ,  B65B51/30 ,  B65B9/06 ,  B65B9/12

CPC分类号： B29C66/83533 ,  B29C65/18 ,  B29C66/1122 ,  B29C66/133 ,  B29C66/4312 ,  B29C66/432 ,  B29C66/71 ,  B29C66/72321 ,  B29C66/72328 ,  B29C66/72341 ,  B29C66/72343 ,  B29C66/81427 ,  B29C66/83421 ,  B29C66/83511 ,  B29C66/83523 ,  B29C66/8491 ,  B65B51/30 ,  B29K2081/04 ,  B29K2075/00 ,  B29K2071/12 ,  B29K2055/02 ,  B29K2033/12 ,  B29K2033/08 ,  B29K2023/12 ,  B29K2023/083 ,  B29K2023/06 ,  B29K2023/04

摘要： A machine for forming filled sealed pouches from a continuously moving sealable web of material includes a first sealing station for forming a longitudinal seal along a side edge of the web, and a horizontal sealing station for forming a plurality of transverse seals in the web. The horizontal sealing station includes a pair of conveyors each carrying jaws that mate along a sealing path to form the transverse seals. A method of forming filled sealed pouches from a continuously moving sealable web of material is also described.

公开(公告)号：US20070169999A1

公开(公告)日：2007-07-26

申请号：US11336039

申请日：2006-01-20

申请人： John MacKlin ,  Robert Foster ,  Paul Bien

发明人： John MacKlin ,  Robert Foster ,  Paul Bien

IPC分类号： F16N7/12

CPC分类号： F16N7/12

摘要： An improved wick-holder for mounting a felt lubricating wick is provided with a passive inertia-activated pump mounted for submersion in the axle-cap lubricant-reservoir at a level that ensures that for all levels of the reservoir, the pump inlet is submerged in the lubricant of the reservoir. In a first embodiment, the passive inertia-activated pump takes the form of a piston-type pump, while in a second embodiment, the passive inertia-activated pump takes the form of a diaphragm pump. In either embodiment, the inlet of the passive inertia-activated pump is submersed in the lubricant-reservoir, and the output of the passive inertia-activated pump is directed to the surface-interface between the felt wick and the axle journal surface-area to increase the lubrication thereof over and above that which normally ensues owing to the conventional capillary action of the felt wick. In a modification of the first embodiment, the piston pump is mounted to a splash sleeve forming part of the wick-holder and which is telescopingly received over the free end of the felt wick, with the output of the passive inertia-activated pump delivering the lubricant to the surface-interface between the felt wick and the axle journal surface-area via the hollow interior-volume of the splash sleeve and wick holder proper. The slosh pump of the invention may replace the wick assembly entirely, whereby the lubrication is achieved solely by the slosh pump of the invention.

摘要翻译： 设置有用于安装毛毡润滑油芯的改进的油绳座设置有被动惯性致动泵，其被安装为浸没在轴套润滑剂储存器中的水平，该水平确保对于储存器的所有水平，泵入口浸没在 蓄水池的润滑剂。 在第一实施例中，被动惯性激活泵采取活塞式泵的形式，而在第二实施例中，被动惯性激活泵采取隔膜泵的形式。 在任一实施例中，被动惯性激活泵的入口浸没在润滑剂储存器中，并且被动惯性激活泵的输出被引导到毡芯和轴颈表面区域之间的表面界面 由于毡芯的常规毛细管作用，增加了其中的润滑性，这超过了常规的润滑。 在第一实施例的变型中，活塞泵安装到形成芯保持器的一部分并且可伸缩地容纳在毡芯的自由端上的飞溅套筒，被动惯性激活泵的输出传递 润滑剂通过飞溅套筒的中空内部容积和芯座固定器到毡芯和轴颈表面区域之间的表面界面。 本发明的冲击泵可以全部更换油绳组件，从而仅通过本发明的冲击泵实现润滑。

公开(公告)号：US20070137940A1

公开(公告)日：2007-06-21

申请号：US11284635

申请日：2005-11-21

申请人： John Macklin ,  Robert Foster ,  Paul Bien

发明人： John Macklin ,  Robert Foster ,  Paul Bien

IPC分类号： F16N7/12

CPC分类号： F16N7/12

摘要： An improved wick-holder for mounting a felt lubricating wick of a lubricating system for lubricating the axle journal surface supported in a support bearing of a traction motor used in a locomotive is provided with a plurality of oil-reservoir louver pockets or splash-cups molded or pressed into the wick holder which store oil from the time that the oil reservoir was full or near its maximum height and which store splashed oil during normal operational use, and delay the run off of the oil to increase the oil saturation of the wick housed within the wick holder. The oil reservoir pockets are located above the minimum serviceable oil level, and are designed to improve and increase oil saturation of the wick. As a result of the increased oil saturation, the wick are, therefore, capable of delivering increased amounts of oil to the axle journal area. The increased amount of oil saturation and reduced wicking height provide increased protection against traction motor support bearing failure if a locomotive is used with axle journal oil levels near the recommended minimum oil levels. Each reservoir pocket or splash cup is preferably louver-shaped, whereby during normal locomotive operating service, the splashing of oil in the bearing housing oil reservoir is collected and delivered to the lubricating wick. In a second embodiment, the reservoir pockets or splash cups are provided on a separate splash sleeve telescopingly mounted over, and to, the lower, free end of the central lubricating wick

摘要翻译： 一种用于安装用于润滑轴颈表面的润滑系统的毡润滑油芯的改进的油绳架，其被支撑在用于机车中的牵引电动机的支撑轴承中，设置有多个油储器百叶窗口袋或成型的飞溅杯 或者从正常操作使用过程中压入从储油器充满或接近其最大高度储存油的油芯夹持器，并延迟油的运行，以增加油箱的油饱和度 在灯芯架内。 储油袋位于最低使用油位之上，旨在改善和提高油芯的油饱和度。 由于油饱和度增加，油芯因此能够将增加量的油输送到轴颈区域。 如果机车与推荐的最低油位附近的轴颈油位一起使用，增加的油饱和度和芯吸高度的降低提高了对牵引电机支撑轴承故障的保护。 每个储存器袋或飞溅杯优选地是百叶窗形状，由此在正常的机车操作服务期间，将油在轴承壳体储油器中的飞溅被收集并输送到润滑油绳。 在第二实施例中，储存袋或飞溅杯设置在可伸缩地安装在中央润滑油芯的下部和下部自由端上的单独的飞溅套筒上

公开(公告)号：US20060174796A1

公开(公告)日：2006-08-10

申请号：US11401177

申请日：2006-04-10

申请人： Paul Bien ,  Robert Foster ,  John Macklin

发明人： Paul Bien ,  Robert Foster ,  John Macklin

IPC分类号： B61F3/00 ,  B61C1/00

CPC分类号： F16C23/041 ,  B61C9/38 ,  F16C17/02 ,  F16C2240/30 ,  F16C2240/40 ,  F16C2326/10

摘要： A contour or profile of a bore of a locomotive traction-motor support bearing structure, where such profile preserves the currently center-loading of the upper load zone but moves inboardly the lower load zone to a more general central location. The profile of the bore for the support bearing, according to the invention, takes into account not only truck-axle bending due to locomotive weight, but also that from motor tilt through bearing clearances, and couple action on the axle from heavy radial loads on PE support bearing and adjacent axle gear. The bore is configured such that the upper surface is horizontal, but the lower surface slopes downwardly in the outboard direction at an angle based on a function dependent upon the three misalignment factors. In a preferred embodiment, the bore mid-section is defined as a frustroconical section of a cone with an altitude having a slope of substantially 1×M1 to the horizontal, and an apex angle of substantially arctan 2×M1, where M1 is the value of the misalignment factor associated with the locomotive load on the axle.

摘要翻译： 机车牵引电动机支撑轴承结构的孔的轮廓或轮廓，其中这种轮廓保持当前中心负载的上部负载区域，但是将下部负载区域内侧移动到更一般的中心位置。 根据本发明的用于支撑轴承的孔的轮廓不仅考虑到机车重量的卡车车身弯曲，而且考虑到从电机通过轴承间隙倾斜，并且从重的径向载荷对轴进行耦合 PE支撑轴承和相邻的轴齿轮。 孔被构造成使得上表面是水平的，但是下表面基于取决于三个不对准因素的函数以一定角度在外侧方向上向下倾斜。 在一个优选实施例中，孔中段被定义为锥体的截头锥形部分，其高度具有与水平方向基本上为1×1m的斜率，基本上为2°M 1的顶角，其中M 1为 与轴上的机车载荷相关的未对准因素。

公开(公告)号：US20050268811A1

公开(公告)日：2005-12-08

申请号：US10862114

申请日：2004-06-03

申请人： Paul Bien ,  Robert Foster ,  John Macklin

发明人： Paul Bien ,  Robert Foster ,  John Macklin

IPC分类号： B61C1/00 ,  B61C9/38 ,  F16C23/04

CPC分类号： F16C23/041 ,  B61C9/38 ,  F16C17/02 ,  F16C2240/30 ,  F16C2240/40 ,  F16C2326/10

摘要： A contour or profile of a bore of a locomotive traction-motor support bearing structure, where such profile preserves the currently center-loading of the upper load zone but moves inboardly the lower load zone to a more general central location. The profile of the bore for the support bearing, according to the invention, takes into account not only truck-axle bending due to locomotive weight, but also that from motor tilt through bearing clearances, and couple action on the axle from heavy radial loads on PE support bearing and adjacent axle gear. The bore is configured such that the upper surface is horizontal, but the lower surface slopes downwardly in the outboard direction at an angle based on a function dependent upon the three misalignment factors. In a preferred embodiment, the bore mid-section is defined as a frustroconical section of a cone with an altitude having a slope of substantially 1×M1 to the horizontal, and an apex angle of substantially arc tan 2×M1, where M1 is the value of the misalignment factor associated with the locomotive load on the axle.

摘要翻译： 机车牵引电动机支撑轴承结构的孔的轮廓或轮廓，其中这种轮廓保持当前中心负载的上部负载区域，但是将下部负载区域内侧移动到更一般的中心位置。 根据本发明的用于支撑轴承的孔的轮廓不仅考虑到机车重量的卡车车身弯曲，而且考虑到从电机通过轴承间隙倾斜，并且从重的径向载荷对轴进行耦合 PE支撑轴承和相邻的轴齿轮。 孔被构造成使得上表面是水平的，但是下表面基于取决于三个不对准因素的函数以一定角度在外侧方向上向下倾斜。 在一个优选实施例中，孔中部被定义为锥体的截头锥形部分，其高度具有与水平方向基本上为1×1m的斜率，并且基本上为弧度2×M 1的顶角，其中M 1为值 与轴上的机车载荷相关的未对准因子。