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1.发明授权公开(公告)号：US06864071B2
公开(公告)日：2005-03-08
申请号：US09865807
申请日：2001-05-25
CPC分类号： C12Q1/6844 , B01J2219/00317 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00614 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10T436/143333 , C12Q2531/113 , C12Q2535/125 , C12Q2565/501
摘要： This invention relates to devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using ligation-dependent strand displacement amplification technologies in combination with bioelectronic microchip technology.
摘要翻译： 本发明涉及使用结合依赖的链置换扩增技术结合生物电子微芯片技术在样品中多重扩增和分析样品中的核酸序列的装置，方法和组合物。
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2.发明授权公开(公告)号：US06238868B1
公开(公告)日：2001-05-29
申请号：US09290577
申请日：1999-04-12
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , B01J2219/00317 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00614 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10T436/143333 , C12Q2531/113 , C12Q2535/125 , C12Q2565/501
摘要： This invention relates to devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using ligation-dependent strand displacement amplification technologies in combination with bioelectronic microchip technology.
摘要翻译： 本发明涉及使用结合依赖的链置换扩增技术结合生物电子微芯片技术在样品中多重扩增和分析样品中的核酸序列的装置，方法和组合物。
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公开(公告)号：US07857957B2
公开(公告)日：2010-12-28
申请号：US11702417
申请日：2007-02-05
申请人： Jing Cheng , Lei Wu , Michael J. Heller , Edward L. Sheldon , Jonathan M. Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
发明人： Jing Cheng , Lei Wu , Michael J. Heller , Edward L. Sheldon , Jonathan M. Diver , James P. O'Connell , Dan Smolko , Shila Jalali , David Willoughby
IPC分类号： G01N27/447 , C12N1/06
CPC分类号： C12Q1/24 , B01L3/502 , B01L2300/0636 , B01L2300/0645 , B01L2300/0877 , B01L2400/0424 , B01L2400/0487 , G01N21/6428
摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。
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