公开(公告)号：US07857957B2

公开(公告)日：2010-12-28

申请号：US11702417

申请日：2007-02-05

申请人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Edward L. Sheldon ,  Jonathan M. Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

发明人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Edward L. Sheldon ,  Jonathan M. Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： G01N27/447 ,  C12N1/06

CPC分类号： C12Q1/24 ,  B01L3/502 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0487 ,  G01N21/6428

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

公开(公告)号：US07172896B2

公开(公告)日：2007-02-06

申请号：US10163835

申请日：2002-06-05

申请人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

发明人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： C12M3/00

CPC分类号： G11C13/0019 ,  B01D57/02 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  C07H21/00 ,  C07K1/045 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6832 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N27/44743 ,  G11C13/0014 ,  G11C13/04 ,  G11C19/00 ,  H01L25/50 ,  H01L2924/0002 ,  C12Q2565/515 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2525/107 ,  C12Q2531/119 ,  C12Q2531/101 ,  C12Q2531/113 ,  H01L2924/00

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

公开(公告)号：US06403367B1

公开(公告)日：2002-06-11

申请号：US09470448

申请日：1999-12-22

申请人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

发明人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Ed Sheldon ,  Jonathan Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： C12M134

CPC分类号： G11C13/0019 ,  B01D57/02 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  C07H21/00 ,  C07K1/045 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6832 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N27/44743 ,  G11C13/0014 ,  G11C13/04 ,  G11C19/00 ,  H01L25/50 ,  H01L2924/0002 ,  C12Q2565/515 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2525/107 ,  C12Q2531/119 ,  C12Q2531/101 ,  C12Q2531/113 ,  H01L2924/00

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

公开(公告)号：US20080047832A1

公开(公告)日：2008-02-28

申请号：US11702417

申请日：2007-02-05

申请人： Jing Cheng ,  Lei Wu ,  Michael Heller ,  Edward Sheldon ,  Jonathan Diver ,  James O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

发明人： Jing Cheng ,  Lei Wu ,  Michael Heller ,  Edward Sheldon ,  Jonathan Diver ,  James O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： C12M1/42

CPC分类号： C12Q1/24 ,  B01L3/502 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0487 ,  G01N21/6428

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

摘要翻译： 我们通过介电电泳在微细电子芯片中进行细胞和癌细胞与外周血液分离。 通过用荧光染料染色细胞核，然后激光诱导荧光成像来检查分离的细胞。 我们还通过电子方式从分离的细胞中释放DNA和RNA，并通过DNA扩增检测特异性标记序列，然后与固定的捕获探针进行电子杂交。 提出了建立“芯片实验室”系统的努力，其中包括选择DNA探针，染料，试剂和完全集成的便携式仪器的原型。

公开(公告)号：US20050271554A1

公开(公告)日：2005-12-08

申请号：US11175195

申请日：2005-07-07

申请人： Shila Jalali ,  Alan Harper ,  Matt Simpson ,  Paul Swanson ,  John Havens

发明人： Shila Jalali ,  Alan Harper ,  Matt Simpson ,  Paul Swanson ,  John Havens

IPC分类号： B01D57/02 ,  B01L3/00 ,  G01N27/447 ,  B01L11/00

CPC分类号： G01N27/44782 ,  B01D57/02 ,  B01L3/5027 ,  G01N27/44717 ,  G01N27/44726 ,  G01N27/44747

摘要： The field of the present invention relates generally to a microstructure apparatus which may be used in a high-throughput screening context to monitor the rate of reaction of an enzyme with its substrate in cases where the product of the reaction has an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatase enzymes, proteases and kinases on charged peptide substrates. The microstructure devices of the present invention comprise a plurality of microstructures, wherein each microstructure comprises a capture matrix located between two electrodes.

摘要翻译： 本发明的领域一般涉及一种微结构装置，其可以在高通量筛选情况下使用，以在反应产物具有改变的净电荷的情况下监测酶与其底物的反应速率。 例如，本文公开的系统和方法可用于检测带电肽底物上的磷酸酶，蛋白酶和激酶的活性。 本发明的微结构器件包括多个微结构，其中每个微结构包括位于两个电极之间的捕获矩阵。

公开(公告)号：US06942778B1

公开(公告)日：2005-09-13

申请号：US09724909

申请日：2000-11-28

申请人： Shila Jalali ,  Alan Harper ,  Matt Simpson ,  Paul Swanson ,  John Havens

发明人： Shila Jalali ,  Alan Harper ,  Matt Simpson ,  Paul Swanson ,  John Havens

IPC分类号： B01D57/02 ,  B01L3/00 ,  G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44782 ,  B01D57/02 ,  B01L3/5027 ,  G01N27/44717 ,  G01N27/44726 ,  G01N27/44747

摘要： The field of the present invention relates generally to a microstructure apparatus which may be used in a high-throughput screening context to monitor the rate of reaction of an enzyme with its substrate in cases where the product of the reaction has an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatase enzymes, proteases and kinases on charged peptide substrates. The microstructure devices of the present invention comprise a plurality of microstructures, wherein each microstructure comprises a capture matrix located between two electrodes.

公开(公告)号：US20050173247A1

公开(公告)日：2005-08-11

申请号：US10980821

申请日：2004-11-04

申请人： Shila Jalali ,  Karla Ewalt ,  Paul Swanson ,  Brian Dwyer ,  Michael Heller ,  John Havens ,  Eugene Tu

发明人： Shila Jalali ,  Karla Ewalt ,  Paul Swanson ,  Brian Dwyer ,  Michael Heller ,  John Havens ,  Eugene Tu

IPC分类号： C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  G01N27/447 ,  G01N33/561 ,  G01N27/453

CPC分类号： G01N33/561 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  G01N27/44717 ,  G01N27/44782

摘要： The present invention relates generally to microtiter plate format devices and methods for separating molecules having different net charges. The devices and methods of the invention are particularly suited for use in high-throughput screening to monitor enzymatic reactions which result in a product having an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatases, proteases and kinases on various peptidic substrates under various conditions.

摘要翻译： 本发明一般涉及用于分离具有不同净电荷的分子的微量滴定板格式装置和方法。 本发明的装置和方法特别适用于高通量筛选以监测酶反应，这导致产品具有改变的净电荷。 例如，本文公开的系统和方法可以用于在各种条件下检测各种肽基底上的磷酸酶，蛋白酶和激酶的活性。