公开(公告)号：US08808982B2

公开(公告)日：2014-08-19

申请号：US12962498

申请日：2010-12-07

Applicant: Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

Inventor： Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

IPC: C12Q1/68 ,  C12N5/071 ,  C12N15/87

CPC classification number: C12N5/0696 ,  A61K38/1709 ,  A61K38/1816 ,  A61K38/44 ,  A61K38/465 ,  A61K38/50 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N9/0075 ,  C12N9/22 ,  C12N9/78 ,  C12N15/117 ,  C12N15/85 ,  C12N15/87 ,  C12N2310/17 ,  C12N2310/335 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12Y114/13039 ,  C12Y301/04012 ,  C12Y305/04004

Abstract: The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.

Abstract translation: 本发明涉及用于改变真核细胞分化状态的方法，所述方法包括将编码一种或多种重编程因子的mRNA引入细胞并将细胞维持在其中细胞存活的条件下，并将导入 表达足够量的细胞并足够的时间产生与引入mRNA的细胞相比表现出改变的分化状态的细胞及其组合物。 例如，本发明提供mRNA分子及其用于将人体细胞重编程为多能干细胞的方法。

公开(公告)号：US20110143397A1

公开(公告)日：2011-06-16

申请号：US12962468

申请日：2010-12-07

Applicant: Katalin Kariko ,  Drew Weissman ,  Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

Inventor： Katalin Kariko ,  Drew Weissman ,  Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

IPC: C12N5/071 ,  C12P21/00

CPC classification number: C12N5/0602 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N5/0696 ,  C12N15/117 ,  C12N15/67 ,  C12N2310/17 ,  C12N2310/33 ,  C12N2310/3341 ,  C12N2501/40

Abstract: The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

Abstract translation: 本发明提供使用包含编码iPS细胞诱导因子的单链mRNA的纯化RNA制备物重编程体细胞的组合物和方法。 纯化的RNA制剂优选基本上不含RNA污染物分子，其可以：i）激活体细胞中的免疫应答，ii）降低体细胞中单链mRNA的表达，和/或iii）活性RNA传感器 在体细胞中。 在某些实施方案中，纯化的RNA制剂基本上不含部分mRNA，双链RNA，未封端的RNA分子和/或单链连续的mRNA。

公开(公告)号：US09012219B2

公开(公告)日：2015-04-21

申请号：US12962468

申请日：2010-12-07

Applicant: Katalin Kariko ,  Drew Weissman ,  Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

Inventor： Katalin Kariko ,  Drew Weissman ,  Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

IPC: C12N5/00 ,  C12N5/02 ,  C12Q1/68 ,  A61K48/00 ,  C07K14/47 ,  C07K14/505 ,  C12N15/117 ,  C12N15/67

CPC classification number: C12N5/0602 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N5/0696 ,  C12N15/117 ,  C12N15/67 ,  C12N2310/17 ,  C12N2310/33 ,  C12N2310/3341 ,  C12N2501/40

Abstract: The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

公开(公告)号：US20110143436A1

公开(公告)日：2011-06-16

申请号：US12962498

申请日：2010-12-07

Applicant: Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

Inventor： Gary Dahl ,  Anthony Person ,  Judith Meis ,  Jerome Jendrisak

IPC: C12N5/00 ,  C07H21/02

CPC classification number: C12N5/0696 ,  A61K38/1709 ,  A61K38/1816 ,  A61K38/44 ,  A61K38/465 ,  A61K38/50 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/47 ,  C07K14/4712 ,  C07K14/505 ,  C12N9/0075 ,  C12N9/22 ,  C12N9/78 ,  C12N15/117 ,  C12N15/85 ,  C12N15/87 ,  C12N2310/17 ,  C12N2310/335 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12Y114/13039 ,  C12Y301/04012 ,  C12Y305/04004

Abstract: The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.

Abstract translation: 本发明涉及用于改变真核细胞分化状态的方法，所述方法包括将编码一种或多种重编程因子的mRNA引入细胞并将细胞维持在其中细胞存活的条件下，并将导入 表达足够量的细胞并足够的时间产生与引入mRNA的细胞相比表现出改变的分化状态的细胞及其组合物。 例如，本发明提供mRNA分子及其用于将人体细胞重编程为多能干细胞的方法。

公开(公告)号：US20060240451A1

公开(公告)日：2006-10-26

申请号：US11352172

申请日：2006-02-09

Applicant: Jerome Jendrisak ,  Judith Meis ,  Ronald Meis ,  Agnes Radek ,  Gary Dahl

Inventor： Jerome Jendrisak ,  Judith Meis ,  Ronald Meis ,  Agnes Radek ,  Gary Dahl

IPC: C12Q1/68 ,  C07H21/02 ,  C12P19/34

CPC classification number: C12N15/1006 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q2521/319

Abstract: The present invention relates to compositions and methods employing 5′-phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5′-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.

Abstract translation: 本发明涉及采用5'-磷酸依赖性核酸外切核酸酶的组合物和方法。 特别地，本发明提供了使用5'-磷酸依赖性核酸外切核酸酶用于选择性富集，分离和扩增特定的一组所需核酸分子的试剂盒和方法，该样品还含有用于各种用途的不想要的核酸分子的样品 。 在优选的实施方案中，所需的核酸分子包含原核和/或真核的mRNA。