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    Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases
    1.
    发明申请
    Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases 审中-公开
    使用5'磷酸依赖性核酸外切核酸酶的组合物和方法

    公开(公告)号：US20060240451A1

    公开(公告)日：2006-10-26

    申请号：US11352172

    申请日：2006-02-09

    申请人： Jerome Jendrisak ,  Judith Meis ,  Ronald Meis ,  Agnes Radek ,  Gary Dahl

    发明人： Jerome Jendrisak ,  Judith Meis ,  Ronald Meis ,  Agnes Radek ,  Gary Dahl

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C12P19/34

    CPC分类号： C12N15/1006 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q2521/319

    摘要： The present invention relates to compositions and methods employing 5′-phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5′-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.

    摘要翻译： 本发明涉及采用5'-磷酸依赖性核酸外切核酸酶的组合物和方法。 特别地，本发明提供了使用5'-磷酸依赖性核酸外切核酸酶用于选择性富集，分离和扩增特定的一组所需核酸分子的试剂盒和方法，该样品还含有用于各种用途的不想要的核酸分子的样品 。 在优选的实施方案中，所需的核酸分子包含原核和/或真核的mRNA。

    Kits and methods for generating 5' capped RNA
    6.
    发明申请
    Kits and methods for generating 5' capped RNA 审中-公开
    用于产生5'封端RNA的试剂盒和方法

    公开(公告)号：US20070281336A1

    公开(公告)日：2007-12-06

    申请号：US11787352

    申请日：2007-04-16

    申请人： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    发明人： Jerome Jendrisak ,  Ronald Meis ,  Gary Dahl

    IPC分类号： C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C12N5/04 ,  C12P21/00 ,  C12N5/10 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12P19/34 ,  C12N9/1007 ,  C12N9/1241 ,  C12N15/1072 ,  C12N15/1075 ,  C12N15/1096 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12Y207/07019 ,  C12Y207/0705

    摘要： The present invention relates to kits and methods for efficiently generating 5′ capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide-capped RNA also provides methods and kits for obtaining only type-specific or condition-specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.

    摘要翻译： 本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种经修饰的核苷酸封端的RNA分子的试剂盒和方法。 本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。 特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。 本发明用于体外生产具有修饰的帽核苷酸的5'上限的RNA，并且通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究，体外或体内产生多肽 ，治疗和商业应用。 本发明还提供用于捕获或分离未包封的RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如在体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。 用于捕获修饰的核苷酸加帽的RNA的方法还提供用于仅通过Cap依赖性减去细胞中捕获的修饰核苷酸加帽的RNA的那部分获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒 与另一类型或病症细胞中的RNA相同的一种类型或病症。 本发明还提供了使用封端酶系统和修饰的帽核苷酸标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的具有5'-二磷酸的初级RNA转录物或RNA。

    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS
    9.
    发明申请
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS 有权
    TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIZING NUCLEIC ACIDS

    公开(公告)号：US20140162897A1

    公开(公告)日：2014-06-12

    申请号：US14148463

    申请日：2014-01-06

    申请人： Haiying Li Grunenwald ,  Nicholas Caruccio ,  Jerome Jendrisak ,  Gary Dahl

    发明人： Haiying Li Grunenwald ,  Nicholas Caruccio ,  Jerome Jendrisak ,  Gary Dahl

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1065 ,  C12N15/10 ,  C12N15/1093 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2521/507

    摘要： The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next-generation sequencing.)

    摘要翻译： 本发明提供了使用转座酶和转座子末端在体外产生双链靶DNA的广泛断裂和5'标记的方法，组合物和试剂盒，然后使用DNA聚合酶产生5'-和3'标记 单链DNA片段，而不进行PCR扩增反应，其中5'-末端的第一个标签显示转移的转座子末端的序列和任选的另外的任意序列，3'-末端的第二个标签显示出 与第一个标签展示的序列不同的序列。 该方法可用于产生用于各种过程的5'-和3'标记的DNA片段，包括环境样品中DNA的宏基因组分析过程，DNA拷贝数变异（CNV）分析和比较基因组测序（ CGS），包括大规模并行DNA测序（所谓的“下一代测序”）。

    Selective 5′ ligation tagging of RNA
    10.
    发明授权
    Selective 5′ ligation tagging of RNA 有权
    RNA的选择性5'连接标记

    公开(公告)号：US08163491B2

    公开(公告)日：2012-04-24

    申请号：US12707243

    申请日：2010-02-17

    申请人： Jerome Jendrisak ,  Ramesh Vaidyanathan ,  Gary Dahl

    发明人： Jerome Jendrisak ,  Ramesh Vaidyanathan ,  Gary Dahl

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q2525/207 ,  C12Q2525/125 ,  C12Q2521/525

    摘要： The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

    摘要翻译： 本发明提供使用RNA 5'多磷酸酶，RNA 5'单磷酸酶，封端酶，脱蛋白酶，核酸焦磷酸酶和RNA连接酶以及其它酶的新型组合物，试剂盒和方法，用于选择性5'连接标记所需类别的 相对于其5'端的特定化学部分不同的RNA分子。 5'标记的RNA分子可用于合成标记的第一支cDNA，双链cDNA和用于各种用途的正义或反义RNA。