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公开(公告)号：US08067164B2
公开(公告)日：2011-11-29
申请号：US12190446
申请日：2008-08-12
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
摘要翻译： 本发明提供了一种用于核酸序列检测的新型阵列方法，其具有改进的特异性，其允许从简单SNP（其中在固定位置发生并且具有有限等位基数的变体）检测遗传变异与更复杂的序列变异模式（例如 与多基因家族或生物体的多种遗传菌株一样，其中各个成员之间的序列变异既不固定也不一致）。 阵列由连接到固体表面的短合成寡核苷酸探针组成，其与单链靶标杂交。 可以使用使用在5'端修饰的引物来防止引入降解未保护链的5'-外切核酸酶降解的方法来产生单链靶标。 本发明还提供用于将寡核苷酸探针固定到阵列表面的印刷缓冲液/溶液。 本发明还提供杂交和洗涤缓冲液和条件以使杂交特异性和信号强度最大化，并减少杂交时间。
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公开(公告)号：US08945928B2
公开(公告)日：2015-02-03
申请号：US13911971
申请日：2013-06-06
申请人： Kerry B Gunning , Mark Aaron Behlke
发明人： Kerry B Gunning , Mark Aaron Behlke
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
摘要翻译： 本发明提供了一种用于核酸序列检测的新型阵列方法，其具有改进的特异性，其允许从简单SNP（其中在固定位置发生并且具有有限等位基数的变体）检测遗传变异与更复杂的序列变异模式（例如 与多基因家族或生物体的多种遗传菌株一样，其中各个成员之间的序列变异既不固定也不一致）。 阵列由连接到固体表面的短合成寡核苷酸探针组成，其与单链靶标杂交。 可以使用使用在5'端修饰的引物来防止引入降解未保护链的5'-外切核酸酶降解的方法来产生单链靶标。 本发明还提供用于将寡核苷酸探针固定到阵列表面的印刷缓冲液/溶液。 本发明还提供杂交和洗涤缓冲液和条件以使杂交特异性和信号强度最大化，并减少杂交时间。
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公开(公告)号：US20150176067A1
公开(公告)日：2015-06-25
申请号：US14576076
申请日：2014-12-18
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
摘要翻译： 本发明提供了一种用于核酸序列检测的新型阵列方法，其具有改进的特异性，其允许从简单SNP（其中在固定位置发生并且具有有限等位基数的变体）检测遗传变异与更复杂的序列变异模式（例如 与多基因家族或生物体的多种遗传菌株一样，其中各个成员之间的序列变异既不固定也不一致）。 阵列由连接到固体表面的短合成寡核苷酸探针组成，其与单链靶标杂交。 可以使用使用在5'端修饰的引物来防止引入降解未保护链的5'-外切核酸酶降解的方法来产生单链靶标。 本发明还提供用于将寡核苷酸探针固定到阵列表面的印刷缓冲液/溶液。 本发明还提供杂交和洗涤缓冲液和条件以使杂交特异性和信号强度最大化，并减少杂交时间。
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公开(公告)号：US20130316927A1
公开(公告)日：2013-11-28
申请号：US13911971
申请日：2013-06-06
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
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公开(公告)号：US20120077712A1
公开(公告)日：2012-03-29
申请号：US13294792
申请日：2011-11-11
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
摘要翻译： 本发明提供了一种用于核酸序列检测的新型阵列方法，其具有改进的特异性，其允许从简单SNP（其中在固定位置发生并且具有有限等位基因数的变体）检测遗传变异与更复杂的序列变异模式（例如 与多基因家族或生物体的多种遗传菌株一样，其中各个成员之间的序列变异既不固定也不一致）。 阵列由连接到固体表面的短合成寡核苷酸探针组成，其与单链靶标杂交。 可以使用使用在5'端修饰的引物来防止引入降解未保护链的5'-外切核酸酶降解的方法来产生单链靶标。 本发明还提供用于将寡核苷酸探针固定到阵列表面的印刷缓冲液/溶液。 本发明还提供杂交和洗涤缓冲液和条件以使杂交特异性和信号强度最大化，并减少杂交时间。
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公开(公告)号：US20090143243A1
公开(公告)日：2009-06-04
申请号：US12190446
申请日：2008-08-12
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
摘要翻译： 本发明提供了一种用于核酸序列检测的新型阵列方法，其具有改进的特异性，其允许从简单SNP（其中在固定位置发生并且具有有限等位基因数的变体）检测遗传变异与更复杂的序列变异模式（例如 与多基因家族或生物体的多种遗传菌株一样，其中各个成员之间的序列变异既不固定也不一致）。 阵列由连接到固体表面的短合成寡核苷酸探针组成，其与单链靶标杂交。 可以使用使用在5'端修饰的引物来防止引入降解未保护链的5'-外切核酸酶降解的方法来产生单链靶标。 本发明还提供用于将寡核苷酸探针固定到阵列表面的印刷缓冲液/溶液。 本发明还提供杂交和洗涤缓冲液和条件以使杂交特异性和信号强度最大化，并减少杂交时间。
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公开(公告)号：US20170369936A1
公开(公告)日：2017-12-28
申请号：US15425882
申请日：2017-02-06
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
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公开(公告)号：US20130040860A1
公开(公告)日：2013-02-14
申请号：US13650087
申请日：2012-10-11
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/0059 , B01J2219/00608 , B01J2219/00626 , B01J2219/00722 , C12P19/34 , C12Q1/6806 , Y10T436/108331 , C12Q2525/125 , C12Q2533/101 , C12Q2521/319
摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
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公开(公告)号：US20090136916A1
公开(公告)日：2009-05-28
申请号：US12191132
申请日：2008-08-13
CPC分类号： C12Q1/701 , B01J2219/00387 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , C12Q1/6881 , C12Q2531/107
摘要： The present invention relates to methods, microarrays and kits for detecting one or more human astrovirus serotypes in a sample (e.g., a fecal sample) from an individual. The method includes amplifying nucleic acid molecules of the sample with one or more primers, to thereby obtain an amplified nucleic acid product; contacting the amplified nucleic acid product with one or more serotype specific probes having a nucleic acid sequence that is specific for only one astrovirus serotype in the group of astroviruses being assessed, wherein the nucleic acid sequence includes between about 9 and 25 nucleic acid bases (e.g., SEQ ID NO: 5-24); and detecting the hybridization complex. The presence of hybridization complexes with a serotype specific probe indicates the presence of one or more specific astrovirus serotypes, and the absence of hybridization complexes with a serotype specific probe indicates the absence of the specific astrovirus serotype. Identification of the astrovirus serotypes allows for one to diagnose an individual infected with the serotype. The present invention further includes microarrays having any one of the astrovirus specific probe, or kits having microarrays and reagents for carrying out the assay.
摘要翻译： 本发明涉及用于检测来自个体的样品（例如，粪便样品）中的一种或多种人星状病毒血清型的方法，微阵列和试剂盒。 该方法包括用一个或多个引物扩增样品的核酸分子，从而获得扩增的核酸产物; 使所述扩增的核酸产物与一种或多种血清型特异性探针接触，所述探针具有在所评估的星状病毒组中仅具有一种星状病毒血清型的核酸序列，其中所述核酸序列包含约9至25个核酸碱基（例如 ，SEQ ID NO：5-24）; 并检测杂交复合物。 与血清型特异性探针的杂交复合物的存在表明存在一种或多种特异性星状病毒血清型，并且与血清型特异性探针不存在杂交复合物表明不存在特异性星状病毒血清型。 识别星状病毒血清型允许一个诊断感染血清型的个体。 本发明还包括具有任何一种星状病毒特异性探针或具有微阵列的试剂盒和用于进行测定的试剂的微阵列。
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10.发明授权Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents 失效底物结合特异性核酸结合剂的杂交率增强公开(公告)号：US06919180B2
公开(公告)日：2005-07-19
申请号：US10104307
申请日：2002-03-22
申请人： Kerry B. Gunning , Tom Powdrill , Michael Hogan
发明人： Kerry B. Gunning , Tom Powdrill , Michael Hogan
IPC分类号： C12N15/09 , C07K5/078 , C07K5/097 , C07K5/117 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B40/10 , G01N33/53
CPC分类号： C12Q1/6832 , B01J2219/00527 , B01J2219/00529 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , C12Q1/6837 , C40B40/06 , C40B40/10 , C12Q2565/501 , C12Q2527/125
摘要： The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
摘要翻译： 本发明涉及用于将核酸与特异性核酸结合剂（例如互补核酸）杂交的试剂盒和方法。 以前，其他人将核酸与结合到底物的这种试剂杂交。 除了该试剂之外，本文所述的改进方法还包括将聚阳离子化的吸引子化合物结合至底物。 合适的多阳离子化吸引子化合物的实例包括多肽，包括具有可调节的可阳离子化氨基酸残基的多肽，例如组氨酸。 公开了利用该杂交速率增强技术的组合物，试剂盒，装置和方法。
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