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    EXO-ENDO CELLULASE FUSION PROTEIN
    3.
    发明申请
    EXO-ENDO CELLULASE FUSION PROTEIN 审中-公开
    EXO-ENDO纤维素酶融合蛋白

    公开(公告)号：US20120135499A1

    公开(公告)日：2012-05-31

    申请号：US13290712

    申请日：2011-11-07

    申请人： Benjamin S. Bower ,  Edmund A. Larenas ,  Colin Mitchinson

    发明人： Benjamin S. Bower ,  Edmund A. Larenas ,  Colin Mitchinson

    IPC分类号： C12N9/42 ,  C12N15/63 ,  C12N1/15 ,  C12N15/62

    CPC分类号： C12N9/2437 ,  C07K2319/00 ,  C07K2319/50 ,  C12N15/625 ,  C12N15/80 ,  C12P21/02 ,  C12P21/06 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.

    摘要翻译： 本发明涉及异源外切纤维素酶融合构建体，其编码具有纤维素分解活性的融合蛋白，其包含衍生自真菌外切纤维二糖水解酶的催化结构域和源自内切葡聚糖酶的催化结构域。 本发明还涉及包含异源外切纤维素酶融合构建体的载体和真菌宿主细胞以及生产纤维素酶融合蛋白和酶纤维素酶组合物的方法。

    Enzyme feed additive and animal feed including it
    7.
    发明授权
    Enzyme feed additive and animal feed including it 有权
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US08715647B2

    公开(公告)日：2014-05-06

    申请号：US11198426

    申请日：2005-08-05

    申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

    发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

    IPC分类号： A23K1/165 ,  A61K38/54 ,  A61K38/47 ,  A61K38/46 ,  A61K38/00

    CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

    摘要： The use is provided composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase.

    摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。

    Beta-Glucosidase Enhanced Filamentous Fungal Whole Cellulase Compositions and Methods Of Use
    9.
    发明申请
    Beta-Glucosidase Enhanced Filamentous Fungal Whole Cellulase Compositions and Methods Of Use 审中-公开
    β-葡萄糖苷酶增强丝状真菌全纤维素酶组合物和使用方法

    公开(公告)号：US20100221784A1

    公开(公告)日：2010-09-02

    申请号：US12676333

    申请日：2008-09-04

    申请人： Meredith K. Fujdala ,  Edmund A. Larenas

    发明人： Meredith K. Fujdala ,  Edmund A. Larenas

    IPC分类号： C12P19/00 ,  C12N9/42

    CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12P19/14 ,  C12Y302/01021

    摘要： The present disclosure provides beta-glucosidase enhanced filamentous fungal whole cellulase compositions. Also provided are methods of hydrolyzing a cellulosic material with beta-glu-cosidase enhanced whole cellulase compositions. The present disclosure further provides methods of decreasing the amount of a whole cellulase required to hydrolyze a cellulosic material by adding an effective amount beta-glucosidase.

    摘要翻译： 本公开提供β-葡糖苷酶增强的丝状真菌全纤维素酶组合物。 还提供了用β-葡糖苷酶增强的全纤维素酶组合物水解纤维素材料的方法。 本公开进一步提供了通过添加有效量的β-葡糖苷酶来减少水解纤维素材料所需的全部纤维素酶的量的方法。

    Enzyme treatment to enhance wettability and absorbency of textiles
    10.
    发明授权
    Enzyme treatment to enhance wettability and absorbency of textiles 失效
    酶处理以增强纺织品的润湿性和吸收性

    公开(公告)号：US6066494A

    公开(公告)日：2000-05-23

    申请号：US952617

    申请日：1998-03-16

    申请人： You-lo Hsieh ,  Mary Michelle Hartzell ,  Matthew G. Boston ,  Kathleen A. Clarkson ,  Katherine D. Collier ,  Thomas P. Graycar ,  Edmund A. Larenas

    发明人： You-lo Hsieh ,  Mary Michelle Hartzell ,  Matthew G. Boston ,  Kathleen A. Clarkson ,  Katherine D. Collier ,  Thomas P. Graycar ,  Edmund A. Larenas

    IPC分类号： D06B21/00 ,  C12S3/02 ,  C12S11/00 ,  D06M16/00 ,  D06M101/32

    CPC分类号： D06M16/003 ,  D06M2101/06 ,  D06M2101/32 ,  D06M2200/00 ,  Y10S8/04

    摘要： Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55.degree. C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25.degree. C.). Contrary to the results with alkaline hydrolysis, the improved water wettability is accompanied by full strength retention. Lipase has also shown to be effective in improving the wetting and absorbent properties of sulfonated polyester and microdenier polyester fabrics.

    摘要翻译： PCT No.PCT / US97 / 03411 Sec。 371日期：1998年3月16日 102（e）1998年3月16日PCT 1997年3月6日PCT公布。 第WO97 / 33001号公报 日期1997年9月12日在没有表面活性剂的情况下用酶处理纺织纤维，具有增加纤维的润湿性和吸收性的作用。 酶是果胶酶，纤维素酶，蛋白酶，脂肪酶或其组合。 通过用纤维素酶和果胶酶的混合物处理，发现棉纤维的润湿性能最显着地提高。 已经研究了五种水解酶对改善几种聚酯织物亲水性的影响。 所研究的五种脂肪中的四种在最佳条件下（3N NaOH在55℃下2小时）改善了常规聚酯织物的水润湿性和吸水性，而不是碱性水解。 与水解水解相比，酶反应在环境温度（25℃）下在更温和的条件下有效，包括较低的浓度（0.01g / L），较短的反应时间（10分钟）。 与碱性水解的结果相反，改善的水润湿性伴随着完全的强度保持。 脂肪酶也显示出有效地改善了磺化聚酯和微米聚酯织物的润湿和吸收性能。