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1.发明申请公开(公告)号：US20080081358A1
公开(公告)日：2008-04-03
申请号：US11862522
申请日：2007-09-27
申请人： PAUL VIITANEN , Carol Mc Cutchen , Xu Li , Mark Emptage , Perry Caimi , Min Zhang , Yat-Chen Chou , Mary Franden
发明人： PAUL VIITANEN , Carol Mc Cutchen , Xu Li , Mark Emptage , Perry Caimi , Min Zhang , Yat-Chen Chou , Mary Franden
摘要： Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
摘要翻译： 当发现含木糖的运动发酵单胞菌菌株在含有木糖的混合糖的培养基中生长时，如果在培养基中包含山梨糖醇或甘露醇，则发现其具有改善的乙醇生产。 在没有生长滞后期的混合糖的浓度以及较高的糖浓度下观察到效果。
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公开(公告)号：US08465961B2
公开(公告)日：2013-06-18
申请号：US13540641
申请日：2012-07-03
申请人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US07629156B2
公开(公告)日：2009-12-08
申请号：US11862522
申请日：2007-09-27
申请人： Paul V. Viitanen , Carol M. Mc Cutchen , Xu Li , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Carol M. Mc Cutchen , Xu Li , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou , Mary Ann Franden
摘要： Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
摘要翻译： 当发现含木糖的运动发酵单胞菌菌株在含有木糖的混合糖的培养基中生长时，如果在培养基中包含山梨糖醇或甘露醇，则发现其具有改善的乙醇生产。 在没有生长滞后期的混合糖的浓度以及较高的糖浓度下观察到效果。
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5.发明申请公开(公告)号：US20110014670A1
公开(公告)日：2011-01-20
申请号：US12641642
申请日：2009-12-18
申请人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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6.发明授权Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas 有权使用木糖利用酵母单体的木糖醇合成突变体的乙醇生产公开(公告)号：US07741084B2
公开(公告)日：2010-06-22
申请号：US11862736
申请日：2007-09-27
申请人： Paul V. Viitanen , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
发明人： Paul V. Viitanen , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
摘要： Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
摘要翻译： 发现使用具有葡萄糖 - 果糖氧化还原酶基因的遗传修饰的具有木糖利用的酵母菌菌株的乙醇的生产由于发酵期间合成的木糖代谢的有害副产物木糖醇的产生大大降低而得到改善。
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7.发明申请ETHANOL PRODUCTION USING XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS 有权使用XYLITOL合成的XYLOSE-ZYMOMONAS合成酶的乙醇生产公开(公告)号：US20080187973A1
公开(公告)日：2008-08-07
申请号：US11862736
申请日：2007-09-27
申请人： PAUL V. VIITANEN , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
发明人： PAUL V. VIITANEN , Carol M. McCutchen , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou
IPC分类号： C12P7/06
摘要： Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
摘要翻译： 发现使用具有葡萄糖 - 果糖氧化还原酶基因的遗传修饰的具有木糖利用的酵母菌菌株的乙醇的生产由于发酵期间合成的木糖代谢的有害副产物木糖醇的产生大大降低而得到改善。
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公开(公告)号：US07067300B2
公开(公告)日：2006-06-27
申请号：US10277249
申请日：2002-10-21
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
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公开(公告)号：US20130177947A1
公开(公告)日：2013-07-11
申请号：US13498069
申请日：2010-09-22
申请人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
发明人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
CPC分类号： C12N9/248 , C12N9/0006 , C12N9/0065 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/2485 , C12P19/00 , C12P19/02 , C12P19/14 , C12Y302/01 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01055 , Y02E50/343
摘要： The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
摘要翻译： 本公开一般涉及糖基水解酶，包含这些酶的组合物，以及使用酶和组合物的方法，例如将纤维素和半纤维素材料糖化为糖。
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公开(公告)号：US20060148053A1
公开(公告)日：2006-07-06
申请号：US11282497
申请日：2006-01-16
申请人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
发明人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
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