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公开(公告)号：US20100310552A1
公开(公告)日：2010-12-09
申请号：US12799282
申请日：2010-04-21
IPC分类号： C12N15/877 , C12P21/00 , C07K16/02 , A61K39/395
CPC分类号： C07K16/00 , A01K67/0275 , A01K2207/15 , A01K2217/052 , A01K2217/206 , A01K2227/30 , A01K2267/01 , C07K14/505 , C07K14/535 , C07K14/56 , C07K14/8135 , C07K2317/11 , C07K2317/23 , C07K2317/92 , C12N9/2462 , C12N9/86 , C12N15/85 , C12N15/8509 , C12N15/87 , C12N2799/027 , C12N2830/15 , C12N2830/90 , C12N2840/203
摘要： The invention relates to transgenic avians which produce antibodies in the egg white by introducing a nucleic acid sequence into the genome of an avian embryo wherein the nucleic acid sequence comprises a nucleotide sequence encoding an antibody and to the antibodies and to methods related thereto.
摘要翻译： 本发明涉及通过将核酸序列引入禽胚胎的基因组中而产生蛋清中的抗体的转基因鸟类，其中所述核酸序列包含编码抗体的核苷酸序列和与抗体相关的方法。
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公开(公告)号：US20100083389A1
公开(公告)日：2010-04-01
申请号：US12387434
申请日：2009-05-01
IPC分类号： A01K67/027 , C12N15/09 , C12N15/13
CPC分类号： C07K16/00 , A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K2317/20 , C07K2317/23 , C12N15/8509 , C12N2799/027 , C12N2800/30 , C12N2830/008 , C12N2830/15 , C12N2830/46 , C12N2830/90 , C12N2840/203
摘要： The invention includes methods of making transgenic avians by introducing a nucleic acid sequence into the genome of an avian embryo wherein the nucleic acid sequence comprises a nucleotide sequence encoding a heterologous protein, developing the avian embryo to hatch, developing the hatched chick to sexual maturity and obtaining offspring from the sexually mature hatched chick which produce egg white containing the heterologous protein.
摘要翻译： 本发明包括通过将核酸序列引入禽胚胎的基因组中来制备转基因鸟类的方法，其中所述核酸序列包含编码异源蛋白质的核苷酸序列，开发禽类胚胎孵化，将孵出的雏鸡发育成性成熟， 从产生含有异源蛋白质的蛋白的性成熟孵出的小鸡获得后代。
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公开(公告)号：US20080145861A1
公开(公告)日：2008-06-19
申请号：US12012387
申请日：2008-02-01
申请人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
发明人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
CPC分类号： C12P21/02
摘要： The present invention relates to cells that can be passaged in culture and can be used for, among other things, promoter assays and the production of heterologous proteins.
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公开(公告)号：US07351582B2
公开(公告)日：2008-04-01
申请号：US11454399
申请日：2006-06-16
申请人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
发明人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
CPC分类号： C12P21/02
摘要： The present invention relates to cells that can be passaged in culture and can be used for, among other things, promoter assays and the production of heterologous proteins.
摘要翻译： 本发明涉及可以在培养物中传代的细胞，并且可以用于促进剂测定和异源蛋白质的生产。
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公开(公告)号：US07432103B2
公开(公告)日：2008-10-07
申请号：US12012387
申请日：2008-02-01
申请人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
发明人： Jeffrey C. Rapp , Stephen H. Parker , Markley C. Leavitt , Dawn M. Eberhardt , Leandro Christmann , Alex J. Harvey
CPC分类号： C12P21/02
摘要： The present invention relates to cells that can be passaged in culture and can be used for, among other things, promoter assays and the production of heterologous proteins.
摘要翻译： 本发明涉及可以在培养物中传代的细胞，并且可以用于促进剂测定和异源蛋白质的生产。
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公开(公告)号：US20090299037A1
公开(公告)日：2009-12-03
申请号：US12536832
申请日：2009-08-06
IPC分类号： C07K1/18
CPC分类号： C07K14/56 , C07K14/505 , C07K14/52 , C07K14/535
摘要： The invention provides methods of purifying proteins, e.g., cytokines, from egg whites.
摘要翻译： 本发明提供从蛋清纯化蛋白质例如细胞因子的方法。
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公开(公告)号：US20080071067A1
公开(公告)日：2008-03-20
申请号：US11901029
申请日：2007-09-14
IPC分类号： C07K1/18
CPC分类号： C07K14/56 , C07K14/505 , C07K14/52 , C07K14/535
摘要： The invention provides methods of purifying proteins, e.g., cytokines, from egg whites.
摘要翻译： 本发明提供从蛋清纯化蛋白质例如细胞因子的方法。
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公开(公告)号：US20080222743A1
公开(公告)日：2008-09-11
申请号：US11799253
申请日：2007-05-01
IPC分类号： C12N15/87 , A01K67/027
CPC分类号： C12N15/1133 , A01K67/0275 , A01K2217/058 , A01K2227/30 , A01K2267/02 , A01K2267/0337 , C12N15/1131 , C12N15/8509 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2799/027
摘要： The invention relates to transgenic avians whose genome contains nucleotide sequences which encode therapeutic polynucleotides that correspond to one or more certain sequences in the genome of an avian pathogen.
摘要翻译： 本发明涉及转基因鸟类，其基因组包含编码对应于禽类病原体基因组中的一个或多个特定序列的治疗性多核苷酸的核苷酸序列。
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9.发明授权Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy 有权使用双光子激光扫描显微镜去除禽类卵母细胞或受精卵的方法公开(公告)号：US07049480B1
公开(公告)日：2006-05-23
申请号：US09654293
申请日：2000-09-01
CPC分类号： C12N15/8509 , A01K67/0273 , A01K2217/05 , A01K2217/075 , A01K2227/30 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N2770/24222
摘要： The invention includes methods of enucleating avian eggs comprising visualizing internal structure of an avian egg utilizing TPLSM and ablating the nucleus of the egg by near-infrared light.
摘要翻译： 本发明包括使鸟类卵切核的方法，包括使用TPLSM观察禽蛋的内部结构，并通过近红外光消除卵的细胞核。
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公开(公告)号：US07312374B2
公开(公告)日：2007-12-25
申请号：US10251364
申请日：2002-09-18
IPC分类号： C12N15/00 , A01K67/027
CPC分类号： A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/20 , C07K2317/23 , C12N15/8509 , C12N2799/027 , C12N2800/30 , C12N2830/008 , C12N2830/15 , C12N2830/46 , C12N2830/90 , C12N2840/203
摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.
摘要翻译： 本发明提供了将异源编码序列稳定引入鸟的基因组并表达编码序列以产生所需蛋白质或改变鸟的表型的方法。 本发明提供了通过细胞质显微注射将转基因导入禽胚胎细胞的细胞质的优选方法。 然后胚胎发育成能够表达异源蛋白质和/或能够通过育种产生一系列转基因鸟类的转基因成体。 本发明还提供了可用于该方法的合成载体和基因启动子，以及表达异源蛋白质和含有异源蛋白质的禽卵的转基因鸟类。
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