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    QUANTITATIVE STANDARD FOR MASS SPECTROMETRY OF PROTEINS
    1.
    发明申请
    QUANTITATIVE STANDARD FOR MASS SPECTROMETRY OF PROTEINS 有权

    公开(公告)号：US20140072991A1

    公开(公告)日：2014-03-13

    申请号：US14009801

    申请日：2012-04-04

    申请人： Matthias Mann ,  Marlis Zeiler ,  Mathias Uhlen ,  Emma Lundberg ,  Werner Straube

    发明人： Matthias Mann ,  Marlis Zeiler ,  Mathias Uhlen ,  Emma Lundberg ,  Werner Straube

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/50 ,  C07K2319/705

    摘要： The present invention provides a method of determining the absolute amount of a target polypeptide in a sample, said method comprising the following steps: (a) adding (aa) a fusion polypeptide to said sample, said fusion polypeptide comprising (i) at least one tag sequence and (ii) a subsequence of the target polypeptide; and (ab) a known absolute amount of a tag polypeptide comprising or consisting of said tag sequence according to (aa) to said sample, wherein said fusion polypeptide on the one hand is mass-altered as compared to said target polypeptide and said tag polypeptide on the other hand, for example, said fusion polypeptide on the one hand and said target polypeptide and said tag polypeptide on the other hand are differently isotope labeled; (b) performing proteolytic digestion of the mixture obtained in step (a); (c) subjecting the result of proteolytic digestion of step (b), optionally after chromatography, to mass spectrometric analysis; and (d) determining the absolute amount of said target polypeptide from (i) the peak intensities in the mass spectrum acquired in step (c) of said fusion polypeptide, said tag polypeptide and said target polypeptide and (ii) said known absolute amount of said tag polypeptide.Furthermore provided is a fusion polypeptide for the quantification of a target polypeptide by mass spectroscopy, wherein: said fusion polypeptide consists of 35 to 455 amino acid residues and comprises (i) a target region, which is a fragment of the target polypeptide, and (ii) a tag region, which is not a fragment of the target polypeptide, said target region consists of 15 to 205 amino acid residues and comprises at least two signature regions; said tag region consists of 20 to 250 amino acid residues and comprises at least two signature regions; and each signature region has the structure Y-Z-X4-28-Y-Z, wherein all Y:s are selected from one of (i)-(iv), wherein (i) is R or K, (ii) is Y, F, W or L, (iii) is E and (iv) is D and each X and each Z are independently any amino acid residue, provided that the Z:s are not P if the Y:s are selected from (i)-(iii); and each signature region comprises at least one amino acid residue comprising a heavy isotope.

    HOT1 AND USES THEREOF
    2.
    发明申请
    HOT1 AND USES THEREOF 审中-公开
    HOT1及其用途

    公开(公告)号：US20130303594A1

    公开(公告)日：2013-11-14

    申请号：US13823431

    申请日：2011-09-14

    申请人： Dennis Kappei ,  Frank Buchholz ,  Matthias Mann ,  Falk Butter

    发明人： Dennis Kappei ,  Frank Buchholz ,  Matthias Mann ,  Falk Butter

    IPC分类号： C07K14/47

    CPC分类号： C07K14/4703 ,  A61K38/00 ,  C07K14/47 ,  C12N15/113 ,  C12N2310/14

    摘要： The invention relates to polynucleotides and polypeptides as defined in the claims for use in the treatment of a disorder associated with abnormal telomerase activity and/or abnormal telomere length in comparison to healthy subjects in a subject. Moreover, the present invention provides a method of identifying an agent capable of decreasing telomerase activity and/or telomere length, and a method of classifying a cancer as a telomerase-negative cancer, as defined in the claims.

    摘要翻译： 本发明涉及权利要求中定义的多核苷酸和多肽，其用于治疗与受试者中的健康受试者相比，与端粒酶活性异常和/或异常端粒长度相关的病症。 此外，本发明提供了鉴定能够降低端粒酶活性和/或端粒长度的试剂的方法，以及如权利要求中所定义的将癌症分类为端粒酶阴性癌症的方法。

    Therapeutic use of antibodies directed against repulsive guidance molecule (RGM)
    5.
    发明授权
    Therapeutic use of antibodies directed against repulsive guidance molecule (RGM) 有权
    针对排斥指导分子（RGM）的抗体的治疗用途

    公开(公告)号：US07981420B2

    公开(公告)日：2011-07-19

    申请号：US10451586

    申请日：2001-12-21

    申请人： Bernhard K. Mueller ,  Philippe P. Monnier ,  Paolo Macchi ,  Friedrich Bonhoeffer ,  Bernd Stahl ,  Matthias Mann ,  Jens S. Anderson

    发明人： Bernhard K. Mueller ,  Philippe P. Monnier ,  Paolo Macchi ,  Friedrich Bonhoeffer ,  Bernd Stahl ,  Matthias Mann ,  Jens S. Anderson

    IPC分类号： A61K39/395 ,  C07K16/28

    CPC分类号： C07K14/47 ,  A61K38/1709 ,  A61K2039/505 ,  C07K14/465 ,  C07K14/4702 ,  C07K16/28

    摘要： The present invention relates to the use of a modulator of a polypeptide having or comprising an amino acid sequence as disclosed herein or of a functional fragment or derivative thereof or of a polynucleotide encoding said polypeptide or fragment or derivative for the preparation of a pharmaceutical composition for preventing, alleviating or treating diseases or conditions associated with the degeneration or injury of vertebrate nervous tissue, associated with seizures or associated with angiogenic disorders or disorders of the cardio-vascular system. Furthermore, the invention provides for the use of a modulator of a polypeptide having or comprising said amino acid sequence of a functional fragment or derivative thereof or of a polynucleotide encoding said polypeptide or fragment or derivative for the preparation of a pharmaceutical composition for preventing, alleviating or treating diseases or conditions associated with the degeneration or injury of vertebrate nervous tissue, associated with angiogenic disorders or disorders of the cardio-vascular system. In addition the invention provides for the use of said polypeptide or said functional fragment or derivative thereof for the preparation of a pharmaceutical composition for preventing or treating tumor growth or formation of tumor metastases or as a marker of stem cells.

    摘要翻译： 本发明涉及具有或包含本文公开的氨基酸序列或其功能片段或衍生物或编码所述多肽或片段或衍生物的多核苷酸的调节剂用于制备药物组合物的用途 预防，减轻或治疗与癫痫相关的脊椎动物神经组织的退化或损伤相关的疾病或病症或与心血管系统的血管生成障碍或病症有关。 此外，本发明提供了具有或包含其功能片段或其衍生物的所述氨基酸序列或编码所述多肽或片段或衍生物的多核苷酸的多肽的调节剂用于制备用于预防，缓解的药物组合物 或治疗与脊髓动物神经组织的变性或损伤相关的疾病或病症，与血管生成障碍或心血管系统的病症有关。 此外，本发明提供了所述多肽或其功能片段或其衍生物用于制备用于预防或治疗肿瘤生长或肿瘤转移形成或作为干细胞标记的药物组合物的用途。

    USE OF RGM AND ITS MODULATORS
    6.
    发明申请
    USE OF RGM AND ITS MODULATORS 审中-公开
    RGM及其调制器的使用

    公开(公告)号：US20110112280A1

    公开(公告)日：2011-05-12

    申请号：US12939823

    申请日：2010-11-04

    申请人： Bernhard K. Mueller ,  Philippe P. Monnier ,  Paolo Macchi ,  Friedrich Bonhoeffer ,  Bernd Stahl ,  Matthias Mann ,  Jens S. Andersen

    发明人： Bernhard K. Mueller ,  Philippe P. Monnier ,  Paolo Macchi ,  Friedrich Bonhoeffer ,  Bernd Stahl ,  Matthias Mann ,  Jens S. Andersen

    IPC分类号： C07K16/00 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/47 ,  A61K38/1709 ,  A61K2039/505 ,  C07K14/465 ,  C07K14/4702 ,  C07K16/28

    摘要： The present invention relates to the use of a modulator of a polypeptide having or comprising an amino acid sequence as disclosed herein or of a functional fragment or derivative thereof or of a polynucleotide encoding said polypeptide or fragment or derivative for the preparation of a pharmaceutical composition for preventing, alleviating or treating diseases or conditions associated with the degeneration or injury of vertebrate nervous tissue, associated with seizures or associated with angiogenic disorders or disorders of the cardiovascular system. Furthermore, the invention provides for the use of a modulator of a polypeptide having or comprising said amino acid sequence of a functional fragment or derivative thereof or of a polynucleotide encoding said polypeptide or fragment or derivative for the preparation of a pharmaceutical composition for preventing, alleviating or treating diseases or conditions associated with the degeneration or injury of vertebrate nervous tissue, associated with angiogenic disorders or disorders of the cardiovascular system. In addition, the invention provides for the use of said polypeptide or said functional fragment or derivative thereof for the preparation of a pharmaceutical compositions for preventing or treating tumor growth or formation of tumor metastases or as a marker of stem cells.

    摘要翻译： 本发明涉及具有或包含本文公开的氨基酸序列或其功能片段或衍生物或编码所述多肽或片段或衍生物的多核苷酸的调节剂用于制备药物组合物的用途 预防，减轻或治疗与癫痫相关的脊髓动物神经组织的退化或损伤相关的疾病或病症，或与心血管系统的血管生成障碍或病症有关。 此外，本发明提供了具有或包含其功能片段或其衍生物的所述氨基酸序列或编码所述多肽或片段或衍生物的多核苷酸的多肽的调节剂用于制备用于预防，缓解的药物组合物 或治疗与血管生成障碍或心血管系统疾病相关的脊椎动物神经组织的退化或损伤相关的疾病或病症。 此外，本发明提供了所述多肽或其功能片段或衍生物用于制备用于预防或治疗肿瘤生长或肿瘤转移形成或作为干细胞标志物的药物组合物的用途。

    ROLLER PRESS, PARTICULARLY FOR INTERPARTICLE COMMINUTION
    7.
    发明申请
    ROLLER PRESS, PARTICULARLY FOR INTERPARTICLE COMMINUTION 审中-公开
    滚动式压力机，特别适用于模块间协调

    公开(公告)号：US20100127110A1

    公开(公告)日：2010-05-27

    申请号：US12373364

    申请日：2007-07-13

    申请人： Alexander Hagedorn ,  Christian Splinter ,  Meinhard Frangenberg ,  Matthias Mann

    发明人： Alexander Hagedorn ,  Christian Splinter ,  Meinhard Frangenberg ,  Matthias Mann

    IPC分类号： B02C4/32 ,  B02C4/02

    CPC分类号： B02C4/02 ,  B02C4/32

    摘要： In order to create a roller press, particularly for comminuting a bed of material, the roller press having two rollers separated from each other by a roller nip and using hydraulic cylinders to press the one roller against the oppositely situated roller over the material situated in the roller nip, the stress on the surface of the rollers to be reduced and their service life to be increased, it is proposed according to the invention that both rollers are designed as loose rollers that are displaceable transversely relative to the roller nip, the bearing housings of the rollers being supported on side parts of the machine frame by hydraulic cylinders that apply the roller compaction pressure.

    摘要翻译： 为了创建辊压机，特别是用于粉碎床层的辊压机，辊压机具有通过辊隙彼此分离的辊，并且使用液压缸将一个辊压靠在相对位置的辊上方位于位于 轧辊辊隙，要减少辊子表面的应力和使用寿命得到提高，根据本发明提出，两个辊子被设计成相对于轧辊辊隙横向移位的松散辊子，轴承座 通过施加辊压作用压力的液压缸支撑在机架的侧部上。

    Method of ionizing atoms or molecules by electrospraying
    9.
    发明授权
    Method of ionizing atoms or molecules by electrospraying 失效
    通过电喷雾电离原子或分子的方法

    公开(公告)号：US5504329A

    公开(公告)日：1996-04-02

    申请号：US401405

    申请日：1995-03-09

    申请人： Matthias Mann ,  Matthias Wilm

    发明人： Matthias Mann ,  Matthias Wilm

    IPC分类号： G01N30/28 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

    CPC分类号： H01J49/165 ,  G01N30/7266 ,  G01N2030/285

    摘要： A method of electrospraying and thereby ionizing an analyte present in a liquid, using a capillary nozzle, in which an electric field is generated at the nozzle to ionise the analyte and produce a divergent beam of charged spray particles. The pressure within the capillary is such that flow of the liquid through the capillary occurs only in the presence of the electric field.

    摘要翻译： 使用毛细管喷嘴电喷雾并从而电离存在于液体中的分析物的方法，其中在喷嘴处产生电场以离子化分析物并产生带电喷雾颗粒的发散束。 毛细管内的压力使得液体通过毛细管的流动仅在存在电场的情况下发生。