Process for producing ethanol
    1.
    发明授权
    Process for producing ethanol 失效
    生产乙醇的方法

    公开(公告)号:US07754456B2

    公开(公告)日:2010-07-13

    申请号:US11628341

    申请日:2005-06-06

    IPC分类号: C12P7/10

    CPC分类号: C12P7/10 Y02E50/16 Y02E50/17

    摘要: A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.

    摘要翻译: 从纤维状木质纤维素原料生产乙醇的方法。 在原料预处理之后,纤维部分首先以高稠度水解,然后将改性材料同时进行纤维素酶持续水解,并在发酵混合物中进行乙醇发酵。 发酵继续将可用纤维素的重要部分转化为乙醇,然后将含有可溶性半纤维素的液体级分加入到发酵混合物中并继续发酵。 通过本发明,可以实现高发酵速率,高乙醇浓度和低乙醇生产成本。

    Mannanase enzymes, genes coding for them and a method for isolating the
genes, as well as a process for bleaching of lignocellulosic pulp
    3.
    发明授权
    Mannanase enzymes, genes coding for them and a method for isolating the genes, as well as a process for bleaching of lignocellulosic pulp 失效
    甘露聚糖酶,编码它们的基因和分离基因的方法,以及木质纤维素浆的漂白方法

    公开(公告)号:US5661021A

    公开(公告)日:1997-08-26

    申请号:US341568

    申请日:1994-11-22

    摘要: The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing. The endomannanase enzyme and enzyme products according to the invention can be used for hydrolyzation of mannopolymers, in particular in connection with bleaching of lignocellulosic pulps.

    摘要翻译: PCT No.PCT / FI93 / 00219 Sec。 371日期:1994年11月22日 102(e)1994年11月22日日期PCT提交1993年5月24日PCT公布。 出版物WO93 / 24622 日期:1993年12月9日本发明涉及一种DNA序列,其编码由木霉属真菌产生并转移到酵母或真菌菌株中的内切葡聚糖酶诱导该菌株产生内切葡聚糖酶,以及分离基因编码方法 内分泌酶。 本发明还涉及含有DNA序列的载体,酵母菌株和真菌菌株。 此外,本发明提供一种含有至少一种含有甘露糖醛酸酶的酶产物,其含有由木霉属真菌产生的至少一种以下的甘露聚糖酶:具有约3.8的甘露聚糖酶活性和等电点(pI)的酶,其具有 甘露聚糖酶活性和约4.1的pI,具有约4.5的甘露聚糖酶活性和pI的酶,具有甘露聚糖酶活性和pI约为5.4的酶和具有约6.5的甘露聚糖酶活性和pI的酶,测定等电点 通过等电聚焦。 根据本发明的内切单体酶和酶产物可用于水解甘露聚合物,特别是与木质纤维素纸浆的漂白有关。