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23 条记录 (耗时 0.027 秒)

    Process for producing ethanol
    1.
    发明授权
    Process for producing ethanol 失效
    生产乙醇的方法

    公开(公告)号：US07754456B2

    公开(公告)日：2010-07-13

    申请号：US11628341

    申请日：2005-06-06

    申请人： Merja Penttilä ,  Matti Siika-Aho ,  Jaana Uusitalo ,  Liisa Viikari

    发明人： Merja Penttilä ,  Matti Siika-Aho ,  Jaana Uusitalo ,  Liisa Viikari

    IPC分类号： C12P7/10

    CPC分类号： C12P7/10 ,  Y02E50/16 ,  Y02E50/17

    摘要： A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.

    摘要翻译： 从纤维状木质纤维素原料生产乙醇的方法。 在原料预处理之后，纤维部分首先以高稠度水解，然后将改性材料同时进行纤维素酶持续水解，并在发酵混合物中进行乙醇发酵。 发酵继续将可用纤维素的重要部分转化为乙醇，然后将含有可溶性半纤维素的液体级分加入到发酵混合物中并继续发酵。 通过本发明，可以实现高发酵速率，高乙醇浓度和低乙醇生产成本。

    Engineering fungi for the utilisation of L-arabinose
    7.
    发明授权
    Engineering fungi for the utilisation of L-arabinose 失效
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US07527951B2

    公开(公告)日：2009-05-05

    申请号：US10257821

    申请日：2002-02-15

    申请人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    IPC分类号： C12P7/06 ,  C12N1/18 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    Manufacture of xylonic acid
    8.
    发明授权
    Manufacture of xylonic acid 有权
    木糖酸的制造

    公开(公告)号：US09315834B2

    公开(公告)日：2016-04-19

    申请号：US13256559

    申请日：2010-03-17

    申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： C12P7/58 ,  C12N9/06 ,  C08B31/00 ,  C12N9/04

    CPC分类号： C12P7/58 ,  C12N9/0006

    摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

    摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质生产木糖酸。

    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION
    9.
    发明申请
    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION 有权
    遗传改良真菌及其在脂生产中的应用

    公开(公告)号：US20130102042A1

    公开(公告)日：2013-04-25

    申请号：US13806281

    申请日：2011-06-21

    申请人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： C12P7/64 ,  C12N15/80

    CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93 ,  C12N15/80 ,  C12P7/6463 ,  C12P7/649 ,  C12Y102/0101 ,  C12Y203/0102 ,  C12Y401/01001 ,  C12Y602/01001 ,  Y02E50/13 ,  Y02P20/52

    摘要： The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

    摘要翻译： 本发明涉及真菌细胞，尤其涉及已被遗传修饰以产生丙酮酸脱氢酶旁路途径的酶以增强其脂质产生的油质真菌细胞。 特别地，细胞被修饰为过度表达编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和/或乙酰辅酶A合成酶（ACS）的基因，任选与编码二酰基甘油酰基转移酶（DAT）的基因一起，或表达编码 PDC与ALD和/或ACS。 还公开了使用改性真菌生产脂质，生物燃料和润滑剂的方法以及在其中有用的表达盒。 还公开了具有磷脂的新型酶：二酰基甘油酰基转移酶（PDAT）活性及其编码它的多核苷酸，其可用于脂质生产。 描述了重组隐球藻细胞及其构建。