公开(公告)号：US3939350A

公开(公告)日：1976-02-17

申请号：US465009

申请日：1974-04-29

申请人： Melvyn N. Kronick ,  William A. Little

发明人： Melvyn N. Kronick ,  William A. Little

IPC分类号： G01N21/64 ,  G01N33/542 ,  G01N33/86 ,  G01T1/20

CPC分类号： G01N21/6428 ,  G01N33/542 ,  G01N33/86 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807

摘要： Receptors are employed to which one or more fluroescent molecules are bound. When the receptors are bound to the epitopic sites on the surface and light of predetermined wave length is directed toward the surface, the fluorescing molecules are activated and fluoresce.In carrying out an assay, receptor is combined with an unknown suspected of containing molecules having the same epitopic sites bound to the surface. The receptor will bind to these molecules reducing the number of receptor sites available for binding to the epitopic sites on the surface. When the assay medium is contacted with the surface, the amount of receptor which binds to the surface, will be a function of available binding sites and, therefore, to the number of the molecules present in the unknown. Upon irradiation of the surface, substantially only the fluoroescent molecules bound to the surface will fluoresce. By monitoring the fluorescence, one can determine the presence and number of molecules of interest present in the unknown.The apparatus consists of a transparent solid sheet, conveniently as part of or optically connected to a prism, a light source set at an angle to provide total internal reflection at the sheet, a cell which includes the reflecting surface as a wall, and a fluorescence detector. Various optics and filters may be employed to modify the light source beam and the fluorescence beam.This work was carried out under a grant of the National Science Foundation.

摘要翻译： 使用一种或多种荧光分子结合的受体。 当受体结合到表面上的表位并且预定波长的光指向表面时，荧光分子被激活并发荧光。

公开(公告)号：US4192996A

公开(公告)日：1980-03-11

申请号：US923844

申请日：1978-07-12

申请人： Melvyn N. Kronick ,  Charles E. Bryson, III ,  John A. Bridgham ,  Sam H. Eletr

发明人： Melvyn N. Kronick ,  Charles E. Bryson, III ,  John A. Bridgham ,  Sam H. Eletr

IPC分类号： G01N21/33 ,  G01J1/42

CPC分类号： G01N21/33

摘要： A device is provided for measuring the concentration of oxygen in a sample utilizing the differential absorption by oxygen (O.sub.2) of two closely spaced ultraviolet (UV) radiation lines. The two UV lines are preferably isotope-shifted Hg lines in the vicinity of the Hg atomic transistion line at 1849.5A.

摘要翻译： 提供了一种用于通过两个紧密间隔的紫外线（UV）辐射线的氧（O 2）的差分吸收来测量样品中的氧浓度的装置。 两条UV线优选在1849.5A的Hg原子转移线附近的同位素偏移的Hg线。

公开(公告)号：US5443791A

公开(公告)日：1995-08-22

申请号：US927254

申请日：1992-08-07

申请人： G. Richard Cathcart ,  Thomas Brennan-Marquez ,  John A. Bridgham ,  George S. Golda ,  Harry A. Guiremand ,  Marianne Hane ,  Louis B. Hoff ,  Eric Lachenmeier ,  Melvyn N. Kronick ,  Douglas H. Keith ,  Paul E. Mayrand ,  Michael L. Metzker ,  William J. Mordan ,  Lincoln J. McBride ,  John Shigeura ,  Chen-Hanson Ting ,  Norman M. Whiteley

发明人： G. Richard Cathcart ,  Thomas Brennan-Marquez ,  John A. Bridgham ,  George S. Golda ,  Harry A. Guiremand ,  Marianne Hane ,  Louis B. Hoff ,  Eric Lachenmeier ,  Melvyn N. Kronick ,  Douglas H. Keith ,  Paul E. Mayrand ,  Michael L. Metzker ,  William J. Mordan ,  Lincoln J. McBride ,  John Shigeura ,  Chen-Hanson Ting ,  Norman M. Whiteley

IPC分类号： B01L3/14 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N21/01 ,  G01N1/10 ,  G01N21/11 ,  G01N35/08

CPC分类号： G01N35/0099 ,  B01L3/50825 ,  G01N35/0098 ,  B01F2215/0037 ,  G01N2035/00079 ,  G01N2035/00237 ,  G01N2035/00356 ,  G01N2035/00435 ,  G01N2035/0403 ,  G01N2035/0475 ,  G01N2035/0491 ,  G01N2035/1013 ,  G01N2035/102 ,  G01N2035/1034 ,  G01N2035/1058 ,  G01N2035/106 ,  G01N35/028 ,  G01N35/1004 ,  G01N35/109 ,  Y10S435/809 ,  Y10T436/119163 ,  Y10T436/2575

摘要： A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for programming and editing. A gauge block registered on the worksurface provides for calibration using the sensing tip, and register cavities on the worksurface provide for modular stations. There is a wash station fop the pipette tip on the worksurface. An automated laboratory based on the liquid-handling system has heating and cooling and a sealable incubation station as well as a magnetic separation station. Methods are disclosed using the apparatus to convey droplets of liquid, to aspirate with minimum tip contamination, to mix liquids in containers, and to validate the worksurface. A duck-billed closure is disclosed for minimizing evaporation and cross-contamination during processing, and is a part of a container disclosed for storing and transporting liquids.

摘要翻译： 液体处理仪器具有工作表面，其具有用于模块化站的注册以支持液体容器，具有连接到感测电路的移液管尖端的移液管装置，用于移动吸头的机器人翻译系统以及具有标识用户界面的控制系统 用于编程和编辑。 在工作表面上注册的量块提供了使用感应尖端的校准，工件表面上的注册腔提供模块化站。 在工作台上有一个洗手台，用于吸取吸头。 基于液体处理系统的自动化实验室具有加热和冷却以及可密封的孵化站以及磁选机。 公开了使用装置输送液体的方法，以最小的尖端污染抽吸以将容器中的液体混合并验证工作面。 公开了一种用于最小化加工期间的蒸发和交叉污染的鸭嘴盖，并且是用于储存和运输液体的容器的一部分。