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公开(公告)号：US07271139B2
公开(公告)日：2007-09-18
申请号：US11474151
申请日：2006-06-23
CPC分类号： C11D3/386 , A21D8/042 , C11D3/38627 , C12N9/20 , C12N15/1027
摘要： The inventors have isolated novel lipolytic enzyme genes with a high homology to the T. lanuginosus lipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
摘要翻译： 本发明人已经分离出与丝状芽孢杆菌脂肪酶基因具有高同源性的新型脂肪分解酶基因，因此非常适合用于基因改组。 因此，本发明提供了一种通过编码脂肪分解酶的两个或更多个同源基因的家族洗牌来产生脂肪分解酶的遗传多样性的方法。 DNA混洗技术用于创建洗牌基因文库，并在合适的表达系统中表达，并筛选表达的蛋白质以进行脂肪分解酶活性。 本发明还提供了包含编码脂肪分解酶和脂肪分解酶（具有脂肪分解酶活性的多肽）的核苷酸序列的多核苷酸。
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2.发明授权Lipolytic enzyme variant with improved stability and polynucleotides encoding same 有权具有改善稳定性的脂肪分解酶变体和编码相同的多核苷酸公开(公告)号：US09163203B2
公开(公告)日：2015-10-20
申请号：US12393512
申请日：2009-02-26
申请人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
发明人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
CPC分类号： C11D3/38627 , C12N9/20 , C12N15/00
摘要： The invention provides lipolytic enzyme variants having improved in-detergent stability and polynucleotides encoding same. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.
摘要翻译： 本发明提供具有改进的洗涤剂稳定性的脂肪分解酶变体和编码它的多核苷酸。 通过用母体脂肪分解酶中的某些特定的氨基酸残基取代具有改善的洗涤剂稳定性的脂肪分解酶变体。
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公开(公告)号：US08679774B2
公开(公告)日：2014-03-25
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20100105092A1
公开(公告)日：2010-04-29
申请号：US12443625
申请日：2007-10-02
申请人： Allan Svendsen , Jesper Vind , Shamkant Anant Patkar , Kim Borch
发明人： Allan Svendsen , Jesper Vind , Shamkant Anant Patkar , Kim Borch
CPC分类号： C12N9/20 , C07K2299/00
摘要： Variants with increased acyl transferase activity can be designed on the basis of a three-dimensional model by making amino acid alterations near the active Ser of lipolytic enzymes such as C. antarctica lipase A or the lipase/acyl transferase from C. parapsilosis.
摘要翻译： 具有增加的酰基转移酶活性的变体可以在三维模型的基础上通过在脂肪分解酶如南极洲洲油脂酶A或来自副杆菌病的脂肪酶/酰基转移酶的活性Ser附近进行氨基酸改变来设计。
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公开(公告)号：US07632669B2
公开(公告)日：2009-12-15
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/20
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20090047384A1
公开(公告)日：2009-02-19
申请号：US12245082
申请日：2008-10-03
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： A21D8/04
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20080131951A1
公开(公告)日：2008-06-05
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/16
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US07282233B2
公开(公告)日：2007-10-16
申请号：US11214188
申请日：2005-08-29
IPC分类号： A21D10/00
CPC分类号： C11D3/38627 , C12N9/20
摘要： Lipolytic enzymes are chemically modified by covalently linking one or more (particularly 1-3) hydrophobic groups to the enzyme molecule. The chemical modification improves the performance of the lipolytic enzyme, e.g., in baking or in detergents.
摘要翻译： 通过将一个或多个（特别是1-3个）疏水基团共价连接到酶分子上来对脂肪分解酶进行化学修饰。 化学修饰改善了脂肪分解酶的性能，例如在烘烤中或在洗涤剂中。
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公开(公告)号：US07396657B2
公开(公告)日：2008-07-08
申请号：US11602553
申请日：2006-11-21
申请人： Signe Munk , Jesper Vind , Kim Borch , Shamkant Anant Patkar , Sanne O. Schroder Glad , Allan Svendsen
发明人： Signe Munk , Jesper Vind , Kim Borch , Shamkant Anant Patkar , Sanne O. Schroder Glad , Allan Svendsen
CPC分类号： C11D3/38627
摘要： Attaching a peptide extension to the C-terminal amino acid of a lipase reduces the tendency to form odor. This may lead to lipase variants with a reduced odor generation when washing textile soiled with fat which includes relatively short-chain fatty acyl groups (e.g., up to C8) such as dairy stains containing butter fat or tropical oils such as coconut oil or palm kernel oil.
摘要翻译： 将肽延伸连接到脂肪酶的C末端氨基酸可降低形成气味的倾向。 当洗涤用脂肪污染的纺织品时，可能导致脂肪酶变体具有减少的气味产生，所述脂肪包括相对短链的脂肪酰基（例如高达C 8 N 8），例如含有脂肪或热带 油如椰子油或棕榈仁油。
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公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞 序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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