公开(公告)号：US08143023B2

公开(公告)日：2012-03-27

申请号：US12521856

申请日：2008-01-25

申请人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

发明人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

IPC分类号： C12P21/06

CPC分类号： C12P21/02 ,  C07K14/39 ,  C12N15/815

摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.

摘要翻译： 描述了在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子表达阳性调节剂，阳性调节剂从 甲醇诱导型启动子，和ii）不加甲醇。

公开(公告)号：US20120142053A1

公开(公告)日：2012-06-07

申请号：US13396925

申请日：2012-02-15

申请人： Noriko Tsutsumi ,  Yuji Terui ,  Shinobu Takagi ,  XiangYu Kong

发明人： Noriko Tsutsumi ,  Yuji Terui ,  Shinobu Takagi ,  XiangYu Kong

IPC分类号： C12P21/00 ,  C07K2/00

CPC分类号： C12P21/02 ,  C07K14/39 ,  C12N15/815

摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.

摘要翻译： 描述了在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子表达阳性调节剂，阳性调节剂从 甲醇诱导型启动子，和ii）不加甲醇。

公开(公告)号：US08236528B2

公开(公告)日：2012-08-07

申请号：US13396925

申请日：2012-02-15

申请人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

发明人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

IPC分类号： C12P21/06

CPC分类号： C12P21/02 ,  C07K14/39 ,  C12N15/815

摘要： A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.

公开(公告)号：US20090311749A1

公开(公告)日：2009-12-17

申请号：US12521856

申请日：2008-01-25

申请人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

发明人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

IPC分类号： C12P21/00

CPC分类号： C12P21/02 ,  C07K14/39 ,  C12N15/815

摘要： The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.

摘要翻译： 本发明涉及在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子，所述阳性调节子 激活甲醇诱导型启动子的转录，以及ii）不添加甲醇。

公开(公告)号：US20100261259A1

公开(公告)日：2010-10-14

申请号：US12376372

申请日：2007-08-03

申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。