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    USE OF TREHALASE TO OBTAIN DROUGHT RESISTANCE IN PLANTS
    1.
    发明申请
    USE OF TREHALASE TO OBTAIN DROUGHT RESISTANCE IN PLANTS 审中-公开
    使用TREHALASE获得植物耐旱性

    公开(公告)号：US20120005774A1

    公开(公告)日：2012-01-05

    申请号：US13148655

    申请日：2010-02-08

    申请人： Patrick van Dijck ,  Lies Vandesteene ,  Nelson Avonce

    发明人： Patrick van Dijck ,  Lies Vandesteene ,  Nelson Avonce

    IPC分类号： A01H1/06

    CPC分类号： C12N15/8273

    摘要： The present invention relates to the use of trehalase to obtain drought and/or salt resistance in plants. More specifically, it relates to genetically modified constructs, transformed into plants and resulting in overexpression of trehalase, whereby the transformed plants show a significantly better drought resistance during the drought period, and a better recovery when water is supplied after the drought period.

    摘要翻译： 本发明涉及海藻糖酶在植物中获得干旱和/或耐盐性的用途。 更具体地说，它涉及转基因植物，转化成植物并导致海藻糖酶的过表达，由此转变的植物在干旱期间显示出更好的抗旱性，并且在干旱期后供水时恢复更好。

    SPECIFIC GENETIC MODIFICATION OF THE ACTIVITY OF TREHALOSE-6-PHOSPHATE SYNTHASE AND EXPRESSION IN A HOMOLOGOUS OR HETEROLOGOUS ENVIRONMENT
    2.
    发明申请
    SPECIFIC GENETIC MODIFICATION OF THE ACTIVITY OF TREHALOSE-6-PHOSPHATE SYNTHASE AND EXPRESSION IN A HOMOLOGOUS OR HETEROLOGOUS ENVIRONMENT 有权
    TREHALOSE-6-PHOSPHATE SYNTH酶活性的特异性遗传修饰及其在异位或异位环境中的表达

    公开(公告)号：US20110296554A1

    公开(公告)日：2011-12-01

    申请号：US12849258

    申请日：2010-08-03

    申请人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    发明人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    IPC分类号： A01H1/06 ,  C12N15/63 ,  C12N1/15 ,  C12Q1/44 ,  C12N15/74

    CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

    摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

    摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。

    Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
    5.
    发明授权
    Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment 有权
    特异性遗传修饰海藻糖-6-磷酸合成酶的活性和在同源或异源环境中的表达

    公开(公告)号：US07781646B2

    公开(公告)日：2010-08-24

    申请号：US11065184

    申请日：2005-02-24

    申请人： Gabriel Iturriaga De La Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    发明人： Gabriel Iturriaga De La Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    IPC分类号： C12N15/82 ,  C12N15/63 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

    摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

    摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。

    Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
    8.
    发明授权
    Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment 有权
    特异性遗传修饰海藻糖-6-磷酸合成酶的活性和在同源或异源环境中的表达

    公开(公告)号：US08796505B2

    公开(公告)日：2014-08-05

    申请号：US12849258

    申请日：2010-08-03

    申请人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  Jose Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    发明人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  Jose Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

    IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

    摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

    摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。