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公开(公告)号：US08859263B2
公开(公告)日：2014-10-14
申请号：US13568900
申请日：2012-08-07
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US06465252B1
公开(公告)日：2002-10-15
申请号：US09599891
申请日：2000-06-21
申请人： Mehmet Toner , Arno W. Tilles , Ulysses J. Balis , Martin L. Yarmush , Maury D. Cosman , Paul A. Dimilla
发明人： Mehmet Toner , Arno W. Tilles , Ulysses J. Balis , Martin L. Yarmush , Maury D. Cosman , Paul A. Dimilla
IPC分类号： C12N500
摘要： The invention features modular cell culturing devices including one or more flat-plate modules, and is based on the discovery that if the flows of liquid medium and oxygenated fluid are separated by a gas-permeable, liquid-impermeable membrane, and the cells are grown attached to the liquid side of the membrane, the device can be used to culture cells with transport of oxygen through the membrane (i.e., direct oxygenation), without regard for the flow rate of the liquid medium passing through the device. The new flow-through cell culturing devices can thus be used to culture cells, e.g., hepatocytes, with high levels of cell function in organ, e.g., liver, assist systems, for production of cells, for production of cell-derived products, such as, proteins or viruses, or for systems to treat biological liquids to remove toxins, such as, ammonia, or add cell-synthesized products, or both.
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公开(公告)号：US20130023041A1
公开(公告)日：2013-01-24
申请号：US13568900
申请日：2012-08-07
IPC分类号： C12M1/34
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US07867752B1
公开(公告)日：2011-01-11
申请号：US09292056
申请日：1999-04-14
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
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公开(公告)号：US20110195055A1
公开(公告)日：2011-08-11
申请号：US12930471
申请日：2011-01-07
IPC分类号： A61K35/12 , C12M1/34 , C12M1/36 , C12N5/071 , C12Q1/02 , C12N5/09 , C12N5/0789 , A61P37/00 , A61P7/00
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且其中细胞可以在环境被动态控制并保持在所需条件下被检查。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US08445261B2
公开(公告)日：2013-05-21
申请号：US12930471
申请日：2011-01-07
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： An apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises an imaging mechanism for automatically determining the state of an individual cell of the cells over time. The imaging mechanism is in communication with the incubating mechanism.
摘要翻译： 用于保持细胞的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于随时间自动确定单元格的单个单元的状态的成像机构。 成像机制与孵化机构相通。
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公开(公告)号：US08241892B2
公开(公告)日：2012-08-14
申请号：US10114892
申请日：2002-04-02
CPC分类号： G01N33/5008 , C12M23/12 , C12M41/36 , C12M41/46 , C12M41/48 , G02B21/16 , G02B21/34 , Y10S435/802 , Y10S435/808 , Y10S435/809 , Y10S435/813
摘要： The present invention pertains to an apparatus for holding cells. The apparatus comprises a mechanism for incubating cells having a dynamically controlled environment in which the cells are grown, which are maintained in a desired condition and in which cells can be examined while the environment is dynamically controlled and maintained in the desired condition. The apparatus also comprises a mechanism for determining the state of the cells. The determining mechanism is in communication with the incubating mechanism. The present invention pertains to a method for holding cells. The method comprises the steps of incubating the cells in a dynamically controlled environment which is maintained in a desired condition and in which the cells can be examined while the environment is dynamically controlled and maintained in the desired condition. Additionally, there is the step of determining the state of the cells.
摘要翻译： 本发明涉及一种用于保持电池的装置。 该装置包括用于孵育具有动态控制的环境的细胞的机制，其中细胞生长，其保持在期望的条件下，并且在环境被动态控制并保持在所需条件下可以检测细胞。 该装置还包括用于确定单元的状态的机构。 确定机制与孵化机制有关。 本发明涉及一种保持细胞的方法。 该方法包括以下步骤：在动态控制的环境中孵育细胞，该环境被保持在期望的状态，并且其中可以在环境被动态地控制并保持在所需条件下细胞被检查。 此外，存在确定细胞状态的步骤。
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公开(公告)号：US07348176B2
公开(公告)日：2008-03-25
申请号：US11057772
申请日：2005-02-14
申请人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
发明人： Paul A. DiMilla , Maury D. Cosman , Rachel Halych , Lisa Romito , Chris Gemmeti , Kevin Odlum
IPC分类号： C12M3/00
摘要： The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a substantially incompressible stop can be adjusted using a gasket that forms a fluid-tight seal between the plates at a plurality of levels of compression. The invention enables the culture of cells between substantially parallel and rigid plates in which a relatively large volume can be used to seed the cells and the holdup volume reduced for perfusion without opening or otherwise disassembling the system to compromise its liquidtightness and sterility. The new closed, modular and scalable cell-culturing chamber can be thus perfused and used to culture cells (e.g., hepatocytes) with high levels of cell function in organ (e.g., liver) assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquids to remove toxins, such as ammonia, add cell-synthesized products, or both.
摘要翻译： 本发明提供了用于培养细胞的模块化室，其中可以调节室的体积而不损害室的密封或不可靠性。 本发明基于这样的原理，即可以使用在多个压缩水平下在板之间形成流体密封的垫圈来调节形成在夹住可压缩垫圈的两个板和基本上不可压缩的止挡件之间的腔室的体积。 本发明使得能够在基本上平行的和刚性的板之间培养细胞，其中可以使用相对较大体积的细胞来种植细胞，并且减少用于灌注的保留体积，而不打开或以其他方式分解系统以损害其液体密度和不育。 因此可以将新的封闭的，模块化和可扩展的细胞培养室灌注并用于培养器官（例如肝）辅助系统中具有高水平细胞功能的细胞（例如肝细胞），用于细胞的产生，用于生产细胞 或衍生的产品，如蛋白质或病毒，或用于处理生物液体以除去毒素（如氨），添加细胞合成产物或两者的系统。
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公开(公告)号：US07169607B2
公开(公告)日：2007-01-30
申请号：US10607695
申请日：2003-06-27
IPC分类号： C12N5/00
CPC分类号： A61K35/407 , A61K35/12 , C12N5/067 , C12N2501/999
摘要： The invention features a liver cell culture comprising hepatocytes that have increased detoxification enzyme activity when isolated from a liver of a donor that had been administered at least one induction agent prior isolation of hepatocyte cells. The induced hepatocytes are used in a bioreactor and cultured to produce hepatocyte cell products or metabolize toxins added to the culture. The bioreactor is, or is an integral part of, a liver assist device used to treat a patient in need of liver assist.
摘要翻译： 本发明的特征在于包含肝细胞的肝细胞培养物，当从肝细胞细胞分离前至少施用至少一种诱导剂的供体肝脏分离时，其具有增强的解毒酶活性。 诱导的肝细胞用于生物反应器并培养以产生肝细胞产物或代谢添加到培养物中的毒素。 生物反应器是用于治疗需要肝辅助的患者的肝辅助装置的一部分或者是其组成部分。
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公开(公告)号：US06759245B1
公开(公告)日：2004-07-06
申请号：US09599891
申请日：2000-06-21
申请人： Mehmet Toner , Arno W. Tilles , Ulysses J. Balis , Martin L. Yarmush , Maury D. Cosman , Paul A. Dimilla
发明人： Mehmet Toner , Arno W. Tilles , Ulysses J. Balis , Martin L. Yarmush , Maury D. Cosman , Paul A. Dimilla
IPC分类号： C12N500
CPC分类号： A61M1/3472 , A61F2/022 , A61M1/3482 , A61M1/3489 , A61M1/3633 , A61M2210/1071 , C12M23/24 , C12M29/04 , C12M29/10 , C12N5/0068 , C12N5/0671
摘要： The invention features modular cell culturing devices including one or more flat-plate modules, and is based on the discovery that if the flows of liquid medium and oxygenated fluid are separated by a gas-permeable, liquid-impermeable membrane, and the cells are grown attached to the liquid side of the membrane, the device can be used to culture cells with transport of oxygen through the membrane (i.e., direct oxygenation), without regard for the flow rate of the liquid medium passing through the device. The new flow-through cell culturing devices can thus be used to culture cells, e.g., hepatocytes, with high levels of cell function in organ, e.g., liver, assist systems, for production of cells, for production of cell-derived products, such as, proteins or viruses, or for systems to treat biological liquids to remove toxins, such as, ammonia, or add cell-synthesized products, or both.
摘要翻译： 本发明的特征在于包括一个或多个平板模块的模块化细胞培养装置，并且基于如下发现：如果液体介质和氧合流体的流动被透气的液体不可渗透膜分离，并且细胞生长 附着于膜的液体侧，该装置可用于通过膜输送氧气（即，直接氧合）来培养细胞，而不考虑通过该装置的液体介质的流速。 因此，新的流通细胞培养装置可用于培养细胞，例如肝细胞，在器官中具有高水平的细胞功能，例如肝，辅助系统，细胞产生，细胞衍生产品的生产，例如 或蛋白质或病毒，或用于处理生物液体以除去毒素（例如氨）或添加细胞合成产物或两者的系统。
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