Quantum dot labeled stem cells for use in providing pacemaker function
    6.
    发明授权
    Quantum dot labeled stem cells for use in providing pacemaker function 有权
    用于提供起搏器功能的量子点标记的干细胞

    公开(公告)号:US08170665B2

    公开(公告)日:2012-05-01

    申请号:US12077970

    申请日:2008-03-21

    IPC分类号: A61N1/00

    摘要: The present invention provides methods and compositions relating to the labeling of target cells with nanometer scale fluorescent semiconductors referred to as quantum dots (QDs). Specifically, a delivery system is disclosed based on the use of negatively charged QDs for delivery of a tracking fluorescent signal into the cytosol of target cells via a passive endocytosis-mediated delivery process. In a specific embodiment of the invention the target cell is a stem cell, preferably a mesenchymal stem cell (MSC). Such labeled MSCs provide a means for tracking the distribution and fate of MSCs that have been genetically engineered to express, for example, a hyperpolarization-activated cyclic nucleotide-gated (“HCN”) channel and administered to a subject to create a biological pacemaker. The invention is based on the discovery that MSCs can be tracked in vitro for up to at least 6 weeks. Additionally, QDs delivered in vivo can be tracked for up to at least 8 weeks, thereby permitting for the first time, the complete 3-D reconstruction of the locations of all MSCs following administration into a host.

    摘要翻译: 本发明提供了与称为量子点(QD)的纳米尺度荧光半导体标记靶细胞有关的方法和组合物。 具体地,基于使用带负电荷的量子点,通过被动内吞作用介导的递送过程将跟踪荧光信号传递到靶细胞的胞质溶胶中,公开了递送系统。 在本发明的具体实施方案中,靶细胞是干细胞,优选间充质干细胞(MSC)。 这种标记的MSC提供了用于跟踪已经被遗传工程化以表达例如超极化激活的环核苷酸门控(“HCN”)信道并且施用于受试者以创建生物起搏器的MSC的分布和命运的手段。 本发明基于这样的发现,MSC可以在体外追踪长达至少6周。 另外,在体内交付的量测数据可以跟踪至少8周,从而允许在管理到主机之后,所有MSC的位置的完整三维重构。