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    Device and method for growing and analyzing cells
    1.
    发明授权
    Device and method for growing and analyzing cells 失效
    用于生长和分析细胞的装置和方法

    公开(公告)号：US08445276B2

    公开(公告)日：2013-05-21

    申请号：US12441117

    申请日：2007-09-10

    申请人： Richard B. Robinson ,  Lev Protas ,  Michael R. Rosen ,  Ira S. Cohen ,  Peter R. Brink

    发明人： Richard B. Robinson ,  Lev Protas ,  Michael R. Rosen ,  Ira S. Cohen ,  Peter R. Brink

    IPC分类号： C12N5/00

    CPC分类号： C12M41/46 ,  C12M23/34 ,  C12M35/08

    摘要： A device and method for analyzing cells includes a housing with a chamber, a barrier supported by a frame disposed within the chamber, and a plate arranged at a bottom surface of the housing interior of the chamber. The plate is adapted to receive and sustain cells and the barrier separates the plate into at least two contiguous separate areas. In some embodiments, a thin rubber strip is arranged at the bottom edge of the barrier, which facilitates control of the area in which each cell type is grown, the size of the gap between the cells, and helps prevents over growth of the two cell types on to each other.

    摘要翻译： 用于分析细胞的装置和方法包括具有腔室的壳体，由设置在腔室内的框架支撑的屏障，以及布置在腔室的壳体内部的底表面处的板。 板适于接收和维持细胞，并且屏障将板分隔成至少两个连续的分离区域。 在一些实施例中，薄的橡胶条布置在阻挡层的底部边缘处，这有助于控制每个细胞类型生长的区域，细胞之间的间隙的大小，并且有助于防止两个细胞的过度生长 彼此之间的类型。

    QUANTUM DOT LABELED STEM CELLS FOR USE IN CARDIAC REPAIR
    6.
    发明申请
    QUANTUM DOT LABELED STEM CELLS FOR USE IN CARDIAC REPAIR 审中-公开
    量子标签干细胞用于心脏修复

    公开(公告)号：US20100158805A1

    公开(公告)日：2010-06-24

    申请号：US12532780

    申请日：2008-03-21

    申请人： Ira S. Cohen ,  Amy Rosen Kontorovich ,  Peter R. Brink ,  Glenn Gaudette ,  Michael R. Rosen ,  Richard B. Robinson

    发明人： Ira S. Cohen ,  Amy Rosen Kontorovich ,  Peter R. Brink ,  Glenn Gaudette ,  Michael R. Rosen ,  Richard B. Robinson

    IPC分类号： A61K49/10 ,  C12N5/071 ,  A61K35/12 ,  A61K9/14 ,  C12N5/077 ,  A61P9/00

    CPC分类号： A61K35/28 ,  A61K49/0067 ,  A61K49/0097 ,  A61K49/0409 ,  A61K49/0423 ,  B82Y5/00

    摘要： The present invention provides methods and compositions relating to the labeling of target cells with quantum dots (QDs). Specifically, a delivery system is disclosed based on the use of negatively charged QDs for delivery of a tracking fluorescent signal into the cytosol of target cells via a passive endocytosis-mediated delivery process. In a specific embodiment of the invention the target cell is a stem cell, preferably a mesenchymal stem cell (MSC). Such labeled MSCs provide a means for tracking the distribution and fate of MSCs that have been administered to a subject to promote cardiac repair. The invention is based on the discovery that MSCs can be tracked in vitro for up to at least 6 weeks. Additionally, QDs delivered in vivo can be tracked for up to at least 8 weeks, thereby permitting for the first time, the complete 3-D reconstruction of the locations of all MSCs following administration into a host.

    摘要翻译： 本发明提供了关于用量子点（QD）标记靶细胞的方法和组合物。 具体地，基于使用带负电荷的量子点，通过被动内吞作用介导的递送过程将跟踪荧光信号传递到靶细胞的胞质溶胶中，公开了递送系统。 在本发明的具体实施方案中，靶细胞是干细胞，优选间充质干细胞（MSC）。 这些标记的MSC提供了跟踪已经施用于受试者以促进心脏修复的MSC的分布和命运的手段。 本发明基于这样的发现，MSC可以在体外追踪长达至少6周。 另外，在体内交付的量测数据可以跟踪至少8周，从而允许在管理到主机之后，所有MSC的位置的完整三维重构。

    Quantum dot labeled stem cells for use in providing pacemaker function
    7.
    发明申请
    Quantum dot labeled stem cells for use in providing pacemaker function 有权
    用于提供起搏器功能的量子点标记的干细胞

    公开(公告)号：US20090062876A1

    公开(公告)日：2009-03-05

    申请号：US12077970

    申请日：2008-03-21

    申请人： Ira S. Cohen ,  Amy B. Rosen ,  Peter R. Brink ,  Glenn Gaudette ,  Michael R. Rosen ,  Richard B. Robinson

    发明人： Ira S. Cohen ,  Amy B. Rosen ,  Peter R. Brink ,  Glenn Gaudette ,  Michael R. Rosen ,  Richard B. Robinson

    IPC分类号： A61N1/362 ,  C12N5/06 ,  A61K49/00 ,  A61P9/00

    CPC分类号： G01N33/588 ,  A61K35/12 ,  A61N1/372 ,  B82Y15/00 ,  C12N5/0663 ,  C12N2510/00 ,  G01N33/6872 ,  G01N2333/705

    摘要： The present invention provides methods and compositions relating to the labeling of target cells with nanometer scale fluorescent semiconductors referred to as quantum dots (QDs). Specifically, a delivery system is disclosed based on the use of negatively charged QDs for delivery of a tracking fluorescent signal into the cytosol of target cells via a passive endocytosis-mediated delivery process. In a specific embodiment of the invention the target cell is a stem cell, preferably a mesenchymal stem cell (MSC). Such labeled MSCs provide a means for tracking the distribution and fate of MSCs that have been genetically engineered to express, for example, a hyperpolarization-activated cyclic nucleotide-gated (“HCN”) channel and administered to a subject to create a biological pacemaker. The invention is based on the discovery that MSCs can be tracked in vitro for up to at least 6 weeks. Additionally, QDs delivered in vivo can be tracked for up to at least 8 weeks, thereby permitting for the first time, the complete 3-D reconstruction of the locations of all MSCs following administration into a host.

    摘要翻译： 本发明提供了与称为量子点（QD）的纳米尺度荧光半导体标记靶细胞有关的方法和组合物。 具体地，基于使用带负电荷的量子点，通过被动内吞作用介导的递送过程将跟踪荧光信号传递到靶细胞的胞质溶胶中，公开了递送系统。 在本发明的具体实施方案中，靶细胞是干细胞，优选间充质干细胞（MSC）。 这种标记的MSC提供了用于跟踪已经被遗传工程化以表达例如超极化激活的环核苷酸门控（“HCN”）信道并且施用于受试者以创建生物起搏器的MSC的分布和命运的手段。 本发明基于这样的发现，MSC可以在体外追踪长达至少6周。 另外，在体内交付的量测数据可以跟踪至少8周，从而允许在管理到主机之后，所有MSC的位置的完整三维重构。