公开(公告)号：US4885357A

公开(公告)日：1989-12-05

申请号：US184348

申请日：1988-04-21

申请人： Brian Larkins ,  Richard E. Cuellar ,  Wallace John C.

发明人： Brian Larkins ,  Richard E. Cuellar ,  Wallace John C.

IPC分类号： C07K14/425 ,  C12N15/82 ,  C12P21/02

CPC分类号： C12N15/8251 ,  C07K14/425 ,  C12N15/8253 ,  C12N15/8254 ,  C12P21/02

摘要： Modified 19kd and 22kd zeins containing lysine are provided. Lysine additions are made by modifying structural genes which encode 19kd and 22kd pre-zeins. Modified zeins produced from the modified structural gene templates are found to retain important properties of unmodified zeins, having similar molecular weights, having the ability to form protein bodies within the rough endoplasmic reticulum of a host cell and being soluble in alcohol.

摘要翻译： 提供了含有赖氨酸的19kd和22kd的玉米蛋白质。 通过修饰编码19kd和22kd前原蛋白的结构基因来制备赖氨酸添加物。 发现由修饰的结构基因模板产生的修饰的玉米醇溶蛋白保留了具有相似分子量的未修饰玉米蛋白的重要性质，具有在宿主细胞的粗糙内质网内形成蛋白质体并且可溶于醇的能力。

公开(公告)号：US5137817A

公开(公告)日：1992-08-11

申请号：US593033

申请日：1990-10-05

申请人： Heinz H. Busta ,  Michael L. Bittner ,  Richard E. Cuellar

发明人： Heinz H. Busta ,  Michael L. Bittner ,  Richard E. Cuellar

IPC分类号： A61N1/04 ,  A61N1/18 ,  C12M3/00 ,  C12N13/00 ,  C12N15/87

CPC分类号： A61N1/0424 ,  A61N1/042 ,  A61N1/0476 ,  A61N1/18 ,  C12M35/02 ,  C12N13/00 ,  C12N15/87

摘要： An electrode set for electrotransformation of a host cell by electroporation, comprising: an electrically insulating substrate; and at least one pair of interdigitated electrodes which are carried by said substrate and which lie in a generally planar array, said electrodes and said substrate being adopted to carry a cell transformant and a host cell thereon, said electrodes and said host cell and said cell transformant being adapted to pass electrical current upon the application of a potential difference across said electrodes.

摘要翻译： 一种用于通过电穿孔电转移宿主细胞的电极组，包括：电绝缘衬底; 以及由所述基板承载并位于大致平面阵列中的至少一对叉指电极，所述电极和所述基板用于承载细胞转化体和宿主细胞，所述电极和所述宿主细胞和所述细胞 转换体适于在施加跨所述电极的电位差时传递电流。

公开(公告)号：US5480805A

公开(公告)日：1996-01-02

申请号：US240496

申请日：1994-05-10

申请人： Fred R. Wolf ,  Richard E. Cuellar

发明人： Fred R. Wolf ,  Richard E. Cuellar

IPC分类号： C12N9/90 ,  C12N15/61 ,  C12N1/19 ,  C12N15/81

CPC分类号： C12N9/90

摘要： A structural gene encoding a polypeptide with .DELTA.8-7 sterol isomerase activity is disclosed, Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed. The structural gene is useful for modulating the accumulation of sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having .DELTA.8-7 sterol isomerase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided.

摘要翻译： 公开了编码具有DELTA 8-7甾醇异构酶活性的多肽的结构基因，用于转化酵母的重组DNA分子和用这种重组DNA分子转化的突变酵母。 结构基因可用于调节酵母中甾醇的积累，包括在具有单或双缺陷的甾醇生物合成酶表达的突变体酵母中增加编码具有DELTA 8-7甾醇异构酶活性的多肽的结构基因的表达水平为 提供。

公开(公告)号：US5365017A

公开(公告)日：1994-11-15

申请号：US119263

申请日：1993-09-09

申请人： Joseph Chappell ,  Court A. Saunders ,  Fred R. Wolf ,  Richard E. Cuellar

发明人： Joseph Chappell ,  Court A. Saunders ,  Fred R. Wolf ,  Richard E. Cuellar

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12N15/82 ,  C12P33/00 ,  A01H1/04 ,  C12N15/00 ,  C12N9/02 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8286

摘要： A method of increasing sterol accumulation in a plant by increasing the copy number of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The copy number is preferably increased by transforming plants with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide. Also disclosed are a method of increasing cycloartenol accumulation in a plant, a method of increasing the resistance of plants to pests and the transformed plants themselves.

公开(公告)号：US4886878A

公开(公告)日：1989-12-12

申请号：US274828

申请日：1988-11-22

申请人： Brian Larkins ,  Richard E. Cuellar ,  John C. Wallace ,  Gad Galili

发明人： Brian Larkins ,  Richard E. Cuellar ,  John C. Wallace ,  Gad Galili

IPC分类号： C07K14/425 ,  C12N15/82 ,  C12P21/02

CPC分类号： C07K14/425 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12P21/02

摘要： Modified 19kd and 22kd zeins containing lysine are provided. Lysine additions are made by modifying structural genes which encode 19kd and 22kd pre-zeins. Modified zeins produced from the modified structural gene templates are found to retain important properties of unmodified zeins, having similar molecular weights, having the ability to form protein bodies within the rough endoplasmic reticulum of a host cell and being soluble in alcohol.

摘要翻译： 提供了含有赖氨酸的19kd和22kd的玉米蛋白质。 通过修饰编码19kd和22kd前原蛋白的结构基因来制备赖氨酸添加物。 发现由修饰的结构基因模板产生的修饰的玉米醇溶蛋白保留了具有相似分子量的未修饰玉米蛋白的重要性质，具有在宿主细胞的粗糙内质网内形成蛋白质体并且可溶于醇的能力。

公开(公告)号：US5306862A

公开(公告)日：1994-04-26

申请号：US596467

申请日：1990-10-12

申请人： Joseph Chappell ,  Court A. Saunders ,  Fred R. Wolf ,  Richard E. Cuellar

发明人： Joseph Chappell ,  Court A. Saunders ,  Fred R. Wolf ,  Richard E. Cuellar

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12N15/82 ,  C12P33/00 ,  A01H1/04 ,  A01H1/02 ,  C12N15/00 ,  C12P7/22

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8286

摘要： A method of increasing sterol accumulation in a plant by increasing the copy number of a gene encoding a polypeptide having HMG-CoA reductase activity is disclosed. The copy number is preferably increased by transforming plants with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity, and a promoter suitable for driving the expression of said polypeptide. Also disclosed are a method of increasing cycloartenol accumulation in a plant, a method of increasing the resistance of plants to pests and the transformed plants themselves.

摘要翻译： 公开了通过增加编码具有HMG-CoA还原酶活性的多肽的基因的拷贝数增加植物中甾醇积累的方法。 优选通过用包含可操作地连接到编码具有HMG-CoA还原酶活性的多肽的外源DNA片段的载体的重组DNA分子和适于驱动所述多肽表达的启动子来转化植物来增加拷贝数。 还公开了增加植物中环戊烯醇积累的方法，增加植物对害虫和转化植物本身的抵抗力的方法。