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11 条记录 (耗时 0.019 秒)

    Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine
    4.
    发明授权
    Method for the extraction of periplasmic proteins from prokaryotic microorganisms in the presence of arginine 失效
    在精氨酸存在下从原核微生物中提取周质蛋白的方法

    公开(公告)号：US5856142A

    公开(公告)日：1999-01-05

    申请号：US906957

    申请日：1997-08-06

    申请人： Richard Legoux ,  Paul Maldonado ,  Marc Salome

    发明人： Richard Legoux ,  Paul Maldonado ,  Marc Salome

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K1/14 ,  C07K14/195 ,  C07K14/41 ,  C07K14/52 ,  C07K14/54 ,  C07K14/61 ,  C12N1/21 ,  C12N15/67 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

    CPC分类号： C07K14/61 ,  C07K1/145 ,  C07K14/5437 ,  C12N15/67 ,  C12P21/02

    摘要： The invention relates to a method for the extraction of recombinant periplasmic proteins wherein arginine is used as the extraction agent. In particular, the invention relates to a method for the extraction of a periplasmic protein of interest, which essentially consists in:(i) suspending the pellet of cells or of cell debris from cells, which cells originate from the culture of a prokaryotic microorganism transformed with an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and(ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.

    摘要翻译： 本发明涉及提取重组质体蛋白质的方法，其中使用精氨酸作为提取剂。 特别地，本发明涉及一种提取目标周质蛋白的方法，其基本上在于：（i）将来自细胞的细胞或细胞碎片的沉淀物悬浮于细胞中，所述细胞源自原核生物微生物转化的培养物 含有编码所述蛋白质的基因的表达载体及其在所述微生物的周质中表达的装置，在含有精氨酸的缓冲溶液中; 和（ii）从由此获得的细菌悬浮液的上清液中回收目标蛋白质。

    Urate oxidase activity protein, recombinant gene coding therefor, expression vector, micro-organisms and transformed cells
    10.
    发明授权
    Urate oxidase activity protein, recombinant gene coding therefor, expression vector, micro-organisms and transformed cells 失效
    Urate氧化酶活性蛋白，编码重组基因，表达载体，微生物和转化细胞

    公开(公告)号：US5382518A

    公开(公告)日：1995-01-17

    申请号：US920519

    申请日：1992-07-28

    申请人： Daniel Caput ,  Pascual Ferrara ,  Jean-Claude Guillemot ,  Mourad Kaghad ,  Richard Legoux ,  Gerard Loison ,  Elisabeth Larbre ,  Johannes Lupker ,  Pascal Leplatois ,  Marc Salome ,  Patrick Laurent

    发明人： Daniel Caput ,  Pascual Ferrara ,  Jean-Claude Guillemot ,  Mourad Kaghad ,  Richard Legoux ,  Gerard Loison ,  Elisabeth Larbre ,  Johannes Lupker ,  Pascal Leplatois ,  Marc Salome ,  Patrick Laurent

    IPC分类号： C07K20060101 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N5/16 ,  C12N9/06 ,  C12N15/53 ,  C12N15/63 ,  C12N9/00 ,  C12P21/00

    CPC分类号： C12N9/0046

    摘要： The invention concerns a new urate oxidase activity protein which has the following sequence:______________________________________ Ser Ala Val Lys Ala Ala Arg Tyr Gly Lys Asp Asn Val Arg Val Tyr Lys Val His Lys Asp Glu Lys Thr Gly Val Gln Thr Val Tyr Glu Met Thr Val Cys Val Leu Leu Glu Gly Glu Ile Glu Thr Ser Tyr Thr Lys Ala Asp Asn Ser Val Ile Val Ala Thr Asp Ser Ile Lys Asn Thr Ile Tyr Ile Thr Ala Lys Gln Asn Pro Val Thr Pro Pro Glu Leu Phe Gly Ser Ile Leu Gly Thr His Phe Ile Glu Lys Tyr Asn His Ile His Ala Ala His Val Asn Ile Val Cys His Arg Trp Thr Arg Met Asp Ile Asp Gly Lys Pro His Pro His Ser Phe Ile Arg Asp Ser Glu Glu Lys Arg Asn Val Gln Val Asp Val Val Glu Gly Lys Gly Ile Asp Ile Lys Ser Ser Leu Ser Gly Leu Thr Val Leu Lys Ser Thr Asn Ser Gln Phe Trp Gly Phe Leu Arg Asp Glu Tyr Thr Thr Leu Lys Glu Thr Trp Asp Arg Ile Leu Ser Thr Asp Val Asp Ala Thr Trp Gln Trp Lys Asn Phe Ser Gly Leu Gln Glu Val Arg Ser His Val Pro Lys Phe Asp Ala Thr Trp Ala Thr Ala Arg Glu Val Thr Leu Lys Thr Phe Ala Glu Asp Asn Ser Ala Ser Val Gln Ala Thr Met Tyr Lys Met Ala Glu Gln Ile Leu Ala Arg Gln Gln Leu Ile Glu Thr Val Glu Tyr Ser Leu Pro Asn Lys His Tyr Phe Glu Ile Asp Leu Ser Trp His Lys Gly Leu Gln Asn Thr Gly Lys Asn Ala Glu Val Phe Ala Pro Gln Ser Asp Pro Asn Gly Leu Ile Lys Cys Thr Val Gly Arg Ser Ser Leu Lys Ser Lys Leu ______________________________________ optionally preceeded by a methionine, or in that it may present a degree of substantial homology with this sequence.The invention is also aimed at medicines containing this protein as well as the genetic engineering implements to obtain it.

    摘要翻译： PCT No.PCT / FR90 / 00532 Sec。 371日期：1991年4月25日 102（e）日期1991年4月25日PCT提交1990年7月13日PCT公布。 公开号WO91 / 00909 1991年1月24日。本发明涉及新的尿酸氧化酶活性蛋白，其具有以下序列： - Ser Ala Val Lys Ala Ala Arg Tyr Gly -Lys Asp Asn Val Arg Val Tyr Lys Val His -Lys Asp Glu Lys Thr Gly Val Gln Thr Val -Tyr Glu Met Thr Val Cys Val Leu Leu Glu -Gly Glu Ile Glu Thr Ser Tyr Thr Lys Ala -Asp Asn Ser Val Ile Val Ala Thr Asp SerIle Lys Asn Thr Ile Tyr Ile Thr Ala Lys -Gln Asn Pro Val Thr Pro Pro Glu Leu Phe -Gly Ser Ile Leu Gly Thr His Phe Ile Glu -Lys Tyr Asn His Ile His Ala Ala His Val-Asn Ile Val Cys His Arg Trp Thr Arg Met -Asp Ile Asp Gly Lys Pro His Pro His Ser -Phe Ile Arg Asp Ser Glu Glu Lys Arg Asn -Val Gln Val Asp Val Val Glu Gly Lys Gly -Ile Asp Ile Lys Ser Ser Leu Ser Gly Leu -Thr Val Leu Lys Ser Thr Asn Ser Gln Phe -Trp Gly Phe Leu Arg Asp Glu Tyr Thr Thr -Leu Lys Glu Thr Trp Asp Arg Ile Leu Ser -Thr Asp Val Asp Ala Thr Trp Gln Trp Lys-Asn Phe Ser Gly Leu Gln Glu Val Arg Ser -His Val Pro Lys Phe Asp Ala Thr Trp Ala -Thr Ala Arg Glu Val Thr Leu Lys Thr Phe -Ala Glu Asp Asn Ser Ala Ser Val Gln Ala -Thr Met Tyr Lys Met Ala Glu Gln Ile Leu -Ala Arg Gln Gln Leu Ile Glu Thr Val Glu -Tyr Ser Leu Pro Asn Lys His Tyr Phe Glu -Ile Asp Leu Ser Trp His Lys Gly Leu Gln-Asn Thr Gly Lys Asn Ala Glu Val Phe Ala -Pro Gln Ser Asp Pro Asn Gly Leu Ile Lys -Cys Thr Val Gly Arg Ser Ser Leu Lys Ser -Lys Leu - 任选地被甲硫氨酸 ，或者它可以呈现与该序列一定程度的基本同源性。 本发明还涉及含有该蛋白质的药物以及获得该蛋白质的遗传工程工具。