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公开(公告)号：US08415155B2
公开(公告)日：2013-04-09
申请号：US12907714
申请日：2010-10-19
申请人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
发明人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
CPC分类号： C12N5/0657 , A61K48/00 , C07H21/04 , C07K14/475 , C07K14/50 , C12N5/0606 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/48 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2510/00
摘要： Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
摘要翻译： 提供了从多能干细胞分化产生心肌细胞的方法和组成。 例如，在某些方面，描述了在ROCK抑制剂存在下将大量悬浮培养物中的多能干细胞分化的方法。 在另外的方面，提供了将干细胞分化为心肌细胞的方法，其克服不同干细胞克隆和不同批次的培养基之间的变异性。
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公开(公告)号：US20120129211A1
公开(公告)日：2012-05-24
申请号：US13276832
申请日：2011-10-19
申请人： STEVEN KATTMAN , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
发明人： STEVEN KATTMAN , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
CPC分类号： C12N5/0696 , C12N5/0607 , C12N2503/00 , C12N2506/00
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括筛选所选批次的培养基中所选干细胞克隆的最佳分化条件的方法，以及使用确定的最佳条件分化成特定细胞谱系。
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公开(公告)号：US20090269314A1
公开(公告)日：2009-10-29
申请号：US12410782
申请日：2009-03-25
申请人： Gordon Keller , Lei Yang , Steven Kattman
发明人： Gordon Keller , Lei Yang , Steven Kattman
CPC分类号： C12N5/0657 , A61K35/12 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/165 , C12N2501/415 , C12N2506/02
摘要： The present invention provides populations of human cardiovascular progenitor cells, methods of making such cells, and methods of using the cells for production of populations of cardiovascular colonies and populations of cardiomyocytes. Methods of cardiomyocytes replacement therapy are also provided.
摘要翻译： 本发明提供人类心血管祖细胞群体，制备此类细胞的方法，以及使用该细胞产生心血管菌落群体和心肌细胞群体的方法。 还提供了心肌细胞替代疗法。
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公开(公告)号：US10077428B2
公开(公告)日：2018-09-18
申请号：US12410782
申请日：2009-03-25
申请人： Gordon Keller , Lei Yang , Steven Kattman
发明人： Gordon Keller , Lei Yang , Steven Kattman
摘要： The present invention provides populations of human cardiovascular progenitor cells, methods of making such cells, and methods of using the cells for production of populations of cardiovascular colonies and populations of cardiomyocytes. Methods of cardiomyocytes replacement therapy are also provided.
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公开(公告)号：US09181529B2
公开(公告)日：2015-11-10
申请号：US13276832
申请日：2011-10-19
申请人： Steven Kattman , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
发明人： Steven Kattman , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
CPC分类号： C12N5/0696 , C12N5/0607 , C12N2503/00 , C12N2506/00
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括筛选所选批次的培养基中所选干细胞克隆的最佳分化条件的方法，以及使用确定的最佳条件分化成特定细胞谱系。
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公开(公告)号：US09034584B2
公开(公告)日：2015-05-19
申请号：US13560159
申请日：2012-07-27
IPC分类号： G01N33/567 , C12Q1/00 , C12N5/077
CPC分类号： C12N5/0657 , C12N2500/25 , C12N2500/32 , C12N2500/33 , C12N2500/36 , C12N2500/90 , C12N2500/99
摘要： The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.
摘要翻译： 本发明涉及用于促进干细胞或从其衍生的糖酵解转化成有氧呼吸及其相关途径（包括氧化磷酸化，TCA循环，脂肪酸氧化和丙酮酸脱羧）的转化方法和组合物。 在具体实施方案中，使用某些培养基来改善特定干细胞衍生细胞（包括人胚胎干细胞和人诱导的多能干细胞）的代谢成熟。 特别的培养基包括不含葡萄糖或任选不含丙酮酸的培养基，脂肪酸与L-肉毒碱，牛磺酸，肌酸，非必需氨基酸，L-谷氨酰胺，以及抗氧化剂或自由基清除剂。
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公开(公告)号：US20130029368A1
公开(公告)日：2013-01-31
申请号：US13560159
申请日：2012-07-27
CPC分类号： C12N5/0657 , C12N2500/25 , C12N2500/32 , C12N2500/33 , C12N2500/36 , C12N2500/90 , C12N2500/99
摘要： The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.
摘要翻译： 本发明涉及用于促进干细胞或从其衍生的糖酵解转化成有氧呼吸及其相关途径（包括氧化磷酸化，TCA循环，脂肪酸氧化和丙酮酸脱羧）的转化方法和组合物。 在具体实施方案中，使用某些培养基来改善特定干细胞衍生细胞（包括人胚胎干细胞和人诱导的多能干细胞）的代谢成熟。 特别的培养基包括不含葡萄糖或任选不含丙酮酸的培养基，脂肪酸与L-肉毒碱，牛磺酸，肌酸，非必需氨基酸，L-谷氨酰胺，以及抗氧化剂或自由基清除剂。
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公开(公告)号：US20110097799A1
公开(公告)日：2011-04-28
申请号：US12907714
申请日：2010-10-19
申请人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
发明人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
CPC分类号： C12N5/0657 , A61K48/00 , C07H21/04 , C07K14/475 , C07K14/50 , C12N5/0606 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/48 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2510/00
摘要： Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
摘要翻译： 提供了从多能干细胞分化产生心肌细胞的方法和组成。 例如，在某些方面，描述了在ROCK抑制剂存在下将大量悬浮培养物中的多能干细胞分化的方法。 在另外的方面，提供了将干细胞分化为心肌细胞的方法，其克服不同干细胞克隆和不同批次的培养基之间的变异性。
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