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公开(公告)号：US09181529B2
公开(公告)日：2015-11-10
申请号：US13276832
申请日：2011-10-19
申请人： Steven Kattman , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
发明人： Steven Kattman , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
CPC分类号： C12N5/0696 , C12N5/0607 , C12N2503/00 , C12N2506/00
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括筛选所选批次的培养基中所选干细胞克隆的最佳分化条件的方法，以及使用确定的最佳条件分化成特定细胞谱系。
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公开(公告)号：US20120129211A1
公开(公告)日：2012-05-24
申请号：US13276832
申请日：2011-10-19
申请人： STEVEN KATTMAN , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
发明人： STEVEN KATTMAN , Wen Bo Wang , Peter Fuhrken , Nathan Meyer , Matthew George , Casey Stankewicz
CPC分类号： C12N5/0696 , C12N5/0607 , C12N2503/00 , C12N2506/00
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括筛选所选批次的培养基中所选干细胞克隆的最佳分化条件的方法，以及使用确定的最佳条件分化成特定细胞谱系。
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公开(公告)号：US20110097799A1
公开(公告)日：2011-04-28
申请号：US12907714
申请日：2010-10-19
申请人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
发明人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
CPC分类号： C12N5/0657 , A61K48/00 , C07H21/04 , C07K14/475 , C07K14/50 , C12N5/0606 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/48 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2510/00
摘要： Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
摘要翻译： 提供了从多能干细胞分化产生心肌细胞的方法和组成。 例如，在某些方面，描述了在ROCK抑制剂存在下将大量悬浮培养物中的多能干细胞分化的方法。 在另外的方面，提供了将干细胞分化为心肌细胞的方法，其克服不同干细胞克隆和不同批次的培养基之间的变异性。
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公开(公告)号：US08415155B2
公开(公告)日：2013-04-09
申请号：US12907714
申请日：2010-10-19
申请人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
发明人： Casey Stankewicz , Matt Riley , Nathaniel Beardsley , Wen Bo Wang , Peter Fuhrken , Steven Kattman
CPC分类号： C12N5/0657 , A61K48/00 , C07H21/04 , C07K14/475 , C07K14/50 , C12N5/0606 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/48 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2510/00
摘要： Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
摘要翻译： 提供了从多能干细胞分化产生心肌细胞的方法和组成。 例如，在某些方面，描述了在ROCK抑制剂存在下将大量悬浮培养物中的多能干细胞分化的方法。 在另外的方面，提供了将干细胞分化为心肌细胞的方法，其克服不同干细胞克隆和不同批次的培养基之间的变异性。
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公开(公告)号：US09487752B2
公开(公告)日：2016-11-08
申请号：US13435698
申请日：2012-03-30
申请人： Nathan Meyer , Matthew George , Casey Stankewicz , Deepika Rajesh
发明人： Nathan Meyer , Matthew George , Casey Stankewicz , Deepika Rajesh
CPC分类号： C12N5/0619 , A61K35/12 , A61K35/30 , C12N5/0622 , C12N2501/119 , C12N2501/155 , C12N2506/45
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGFβ. As an advantage, the neural cells may be provided with improved consistency and purity.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括在基本上不含生长因子如FGF和TGFβ的培养基中用于神经分化的引发干细胞的方法。 作为优点，神经细胞可以具有改善的一致性和纯度。
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6.发明申请公开(公告)号：US20120276063A1
公开(公告)日：2012-11-01
申请号：US13435698
申请日：2012-03-30
申请人： Nathan Meyer , Matthew George , Casey Stankewicz , Deepika Rajesh
发明人： Nathan Meyer , Matthew George , Casey Stankewicz , Deepika Rajesh
CPC分类号： C12N5/0619 , A61K35/12 , A61K35/30 , C12N5/0622 , C12N2501/119 , C12N2501/155 , C12N2506/45
摘要： Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGFβ. As an advantage, the neural cells may be provided with improved consistency and purity.
摘要翻译： 提供了多能干细胞分化的方法和组成。 例如，在某些方面，包括在基本上不含生长因子例如FGF和TGF-bgr的培养基中用于神经分化的引发干细胞的方法。 作为优点，神经细胞可以具有改善的一致性和纯度。
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7.发明申请Metal ion mediated fluorescence superquenching assays, kits and reagents 审中-公开金属离子介导的荧光超强化测定，试剂盒和试剂公开(公告)号：US20070238143A1
公开(公告)日：2007-10-11
申请号：US11219673
申请日：2005-09-07
申请人： Wensheng Xia , Frauke Rininsland , Sriram Kumaraswamy , Stuart Kushon , Liangde Lu , Xiaobo Shi , Casey Stankewicz , Shannon Wittenburg , Komandoor Achyuthan , Duncan McBranch , David Whitten
发明人： Wensheng Xia , Frauke Rininsland , Sriram Kumaraswamy , Stuart Kushon , Liangde Lu , Xiaobo Shi , Casey Stankewicz , Shannon Wittenburg , Komandoor Achyuthan , Duncan McBranch , David Whitten
CPC分类号： G01N33/542 , C12Q1/42 , C12Q1/44 , C12Q1/485 , G01N2333/912 , G01N2333/916 , G01N2333/95
摘要： Reagents and assays for kinase, phosphatase and protease enzyme activity which employ metal ion-phosphate ligand specific binding and fluorescent polymer superquenching are described. The assays provide a general platform for the measurement of kinase, phosphatase and protease enzyme activity using peptide and protein substrates. Reagents and assays based on DNA hybridization and reagents and assays for proteins which employ aptamers, antibodies and other ligands are also described.
摘要翻译： 描述了使用金属离子 - 磷酸盐配体特异性结合和荧光聚合物超强化的激酶，磷酸酶和蛋白酶活性的试剂和测定法。 测定提供了使用肽和蛋白质底物测量激酶，磷酸酶和蛋白酶活性的通用平台。 还描述了基于DNA杂交的试剂和测定以及使用适体，抗体和其它配体的蛋白质的试剂和测定法。
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8.发明申请Metal mediated fluorescence superquenching assays for detection of phosphorylation and dephosphorylation of quencher-labeled substrates 审中-公开金属介导的荧光超强化测定法用于检测猝灭剂标记的底物的磷酸化和去磷酸化公开(公告)号：US20060292653A1
公开(公告)日：2006-12-28
申请号：US11378470
申请日：2006-03-20
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N33/92
摘要： A method includes contacting an analyte; a phosphorylatable or dephosphorylatable substrate bound to a quencher; and a sensor that includes at least one fluorescent polymer complexed to one or more metal species; to form a composition; and determining the fluorescence of the composition, if any; wherein when the substrate is phosphorylated, an association occurs between the substrate and the sensor such that fluorescent superquenching occurs between the fluorescent polymer and the quencher.
摘要翻译： 一种方法包括使分析物接触; 与猝灭剂结合的可磷酸化或可去磷酸化的底物; 以及传感器，其包括与一种或多种金属物质复合的至少一种荧光聚合物; 形成组合物; 并确定组合物的荧光（如果有的话） 其中当衬底被磷酸化时，在衬底和传感器之间发生关联，使得在荧光聚合物和猝灭剂之间发生荧光超淬火。
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