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1.发明授权Glutaryl cephalosporin amidase from Pseudomonas diminuta BS-203 失效来自Pseudomonas diminuta BS-203的甲基头孢菌素酰胺酶公开(公告)号：US06528300B2
公开(公告)日：2003-03-04
申请号：US09957960
申请日：2001-09-21
申请人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
发明人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
IPC分类号： C12N980
摘要： The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
摘要翻译： 本发明提供了来自减毒假单胞菌菌株BS-203的戊二酰7-ACA酰胺酶，其催化7-β-（4-羧基丁酰氨基） - 头孢烷酸至7-氨基头孢烷酸和戊二酸的水解。 本发明还提供了可用于生产该酰胺酶的核酸序列，载体和宿主细胞。 戊二酰7-ACA酰胺酶可用于制备7-氨基头孢烷酸。
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公开(公告)号：US20050208620A1
公开(公告)日：2005-09-22
申请号：US10915172
申请日：2004-08-09
申请人： Jonathan Basch , Shu-Jen Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
发明人： Jonathan Basch , Shu-Jen Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
CPC分类号： C12N9/0077 , C07K14/79 , C07K2299/00 , C12P17/181
摘要： Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.
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3.发明授权Glutaryl cephalosporin amidase from Pseudomonas diminuta BS-203 失效来自Pseudomonas diminuta BS-203的甲基头孢菌素酰胺酶公开(公告)号：US06942990B2
公开(公告)日：2005-09-13
申请号：US10346662
申请日：2003-01-17
申请人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
发明人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
IPC分类号： C12N15/09 , C07K14/21 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/80 , C12N15/31 , C12P35/02 , C12R1/38 , C12P35/04 , A01N43/20 , C12N1/20
摘要： The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-β-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
摘要翻译： 本发明提供了来自减毒假单胞菌菌株BS-203的戊二酰7-ACA酰胺酶，其催化7-β-（4-羧基丁酰氨基） - 头孢烷酸至7-氨基头孢烷酸和戊二酸的水解。 本发明还提供了可用于生产该酰胺酶的核酸序列，载体和宿主细胞。 戊二酰7-ACA酰胺酶可用于制备7-氨基头孢烷酸。
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公开(公告)号：US06884608B2
公开(公告)日：2005-04-26
申请号：US10321188
申请日：2002-12-17
申请人： Jonathan David Basch , Shu-Jen David Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
发明人： Jonathan David Basch , Shu-Jen David Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
CPC分类号： C12N9/0077 , C07K14/79 , C07K2299/00 , C12P17/181
摘要： Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.
摘要翻译： 提供了分离的核酸序列和由此编码的用于埃坡霉素B羟化酶及其突变体和变体以及位于埃坡霉素B羟化酶基因下游的铁氧还蛋白的多肽。 还提供了含有这些载体的载体和细胞。 此外，提供了用于制备重组微生物的方法，使用这些重组微生物制备含有羟烷基的埃坡霉素的方法和由埃坡霉素B羟化酶的突变体产生的埃坡霉素类似物。
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公开(公告)号：US08158394B2
公开(公告)日：2012-04-17
申请号：US12756700
申请日：2010-04-08
CPC分类号： C12N9/0008 , C12N9/0018
摘要： Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.
摘要翻译： 提供了用于表达甲酸脱氢酶（FDH）和改性苯丙氨酸脱氢酶（PDHmod）的双顺反子质粒。
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公开(公告)号：US20100261236A1
公开(公告)日：2010-10-14
申请号：US12756700
申请日：2010-04-08
CPC分类号： C12N9/0008 , C12N9/0018
摘要： Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.
摘要翻译： 提供了用于表达甲酸脱氢酶（FDH）和改性苯丙氨酸脱氢酶（PDHmod）的双顺反子质粒。
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公开(公告)号：US07214527B2
公开(公告)日：2007-05-08
申请号：US10915172
申请日：2004-08-09
申请人： Jonathan David Basch , Shu-Jen David Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
发明人： Jonathan David Basch , Shu-Jen David Chiang , Suo-Win Liu , Akbar Nayeem , Yuhua Sun , Li You
CPC分类号： C12N9/0077 , C07K14/79 , C07K2299/00 , C12P17/181
摘要： Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.
摘要翻译： 提供了分离的核酸序列和由此编码的用于埃坡霉素B羟化酶及其突变体和变体以及位于埃坡霉素B羟化酶基因下游的铁氧还蛋白的多肽。 还提供了含有这些载体的载体和细胞。 此外，提供了用于制备重组微生物的方法，使用这些重组微生物制备含有羟烷基的埃坡霉素的方法和由埃坡霉素B羟化酶的突变体产生的埃坡霉素类似物。
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