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公开(公告)号：US20170096686A1
公开(公告)日：2017-04-06
申请号：US15314191
申请日：2015-05-27
Applicant: Tyton BioSciences, LLC
Inventor： Vyacheslav Adrianov , Igor Kostenyuk , Peter Majeranowski , Iulian Bobe , Mintu K. Desai
IPC: C12P7/06 , C07K14/415 , C12N9/10 , C12N15/82
CPC classification number: C12P7/06 , C07K14/415 , C12N9/1092 , C12N15/8226 , C12N15/8227 , C12N15/8245 , C12N15/8246 , C12N15/8275 , C12Y205/01019 , Y02E50/17
Abstract: Genetically modified tobacco plants are provided having altered hexose accumulation. Methods are provided for producing ethanol from fermentation of tobacco biomass derived from the tobacco plants having altered hexose accumulation. The altered hexose accumulation can be an increase in total hexose content or an increase in hexose content in the phloem or the roots/shoots as compared to non-genetically modified tobacco plants. Expression vectors are provided for tobacco plant transformation having a gene encoding a sucrose invertase inhibitor operably linked to a promoter, such that expression of the inhibitor in the plant can increase and/or alter hexose accumulation in the plant. The genetically modified tobacco plants having altered hexose accumulation can further contain a transgenic construct to confer resistance to a glyphosate herbicide or a phosphinothricin (PPT) herbicide.
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公开(公告)号：US20150344978A1
公开(公告)日：2015-12-03
申请号：US14723397
申请日：2015-05-27
Applicant: Tyton Biosciences, LLC
Inventor： Hsi-Chuan Chen , Peter Majeranowski , Igor Kostenyuk , Iulian Bobe
Abstract: Compositions and methods are provided for enhanced production of ethanol from fermentation of tobacco biomass. Nicotine resistant microorganisms are provided, as well as methods for making these nicotine resistant microorganisms. A biologically pure culture is provided of a nicotine resistant Saccharomyces cerevisiae strain or a mutant thereof having all the identifying characteristics thereof. Methods are provided for producing ethanol from fermentation of tobacco biomass in which the nicotine resistant microorganisms are used in the fermentation of the tobacco biomass, wherein a higher amount of ethanol can be produced from the fermentation. The nicotine resistant yeast strains of the present disclosure can improve ethanol production in tobacco biomass extract fermentations and shorten the fermentation time.
Abstract translation: 提供了组合物和方法，用于从烟草生物质发酵中增强生产乙醇。 提供了尼古丁抗性微生物，以及制备这些耐受尼古丁的微生物的方法。 提供具有所有鉴定特征的尼古丁耐药酿酒酵母菌株或其突变体的生物学纯培养物。 提供了用于从烟草生物质的发酵中生产乙醇的方法，其中在烟草生物质的发酵中使用尼古丁抗性微生物，其中可以从发酵产生更高量的乙醇。 本公开的尼古丁耐药酵母菌株可以改善烟草生物质提取物发酵中的乙醇生产并缩短发酵时间。
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公开(公告)号：US20190276983A1
公开(公告)日：2019-09-12
申请号：US16424899
申请日：2019-05-29
Applicant: Tyton Biosciences, LLC
Inventor： Iulian BOBE , Florin G. BARLA , Hsun-Cheng SU , Conor Hartman
Abstract: The presently disclosed subject matter relates to a method and system of providing customized bioprocessing conditions for a variety of feedstock materials. Particularly, it has been surprisingly found that there is a correlation between the weight percent of lignin, cellulose, and/or hemicellulose and various reaction conditions. The weight percent lignin, cellulose, and hemicellulose are used to calculate indexes used to create a process index for sorting the feedstock into categories. A process adjustment factor can also be calculated and used to adjust the reaction conditions for each feedstock material.
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公开(公告)号：US20190218362A1
公开(公告)日：2019-07-18
申请号：US16246044
申请日：2019-01-11
Applicant: Tyton Biosciences, LLC
Inventor： Florin G. Barla , Todd Showalter , Hsun-Cheng Su , Jeremy Jones , Iulian Bobe
IPC: C08J11/26 , C08L1/02 , C07C63/26 , D06M13/184
CPC classification number: C08J11/26 , C07C63/26 , C08J2367/02 , C08J2401/02 , C08L1/02 , D06M13/184
Abstract: Systems and methods are provided that involve a subcritical water reaction to recycle the cellulose and polyester components of waste cotton and cotton/polyester blend textiles that would otherwise be discarded or disposed of. Specifically, the disclosed methods provide for treatment of the waste textiles to produce advanced materials including cellulose and terephthalic acid (TPA) with a low environmental impact. The cellulose and TPA that are produced are of a high quality allowing for production of regenerated cellulose and regenerated polyethylene terephthalate (PET) suitable for fiber spinning and textile applications.
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公开(公告)号：US20170298459A1
公开(公告)日：2017-10-19
申请号：US15637386
申请日：2017-06-29
Applicant: Tyton Biosciences, LLC
Inventor： Sandeep KUMAR , Jose Luis Garcia MOSCOSO , Iulian BOBE , Peter MAJERANOWSKI
CPC classification number: C13K1/04 , A24B7/00 , A24B15/24 , A24B15/243 , C07D401/04 , C08H8/00 , C11B1/10 , C13K1/02
Abstract: Processes for recovering sugars and nicotine from a tobacco biomass include feeding a biomass of tobacco plants and subcritical water to a reactor, hydrolyzing the biomass of tobacco plants with the subcritical water at a temperature between about 150° C. and 305° C. and recovering a liquid product and a solid product from the reactor, wherein the liquid product contains water-soluble sugars and nicotine.
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Patent Agency Ranking
公开(公告)号：US09765411B2
公开(公告)日：2017-09-19
申请号：US14270839
申请日：2014-05-06
Applicant: TYTON BIOSCIENCES, LLC
Inventor： Sandeep Kumar , Jose Luis Garcia Moscoso , Iulian Bobe , Peter Majeranowski
IPC: C13K1/02 , C07D401/04 , A24B15/24
CPC classification number: C13K1/04 , A24B7/00 , A24B15/24 , A24B15/243 , C07D401/04 , C08H8/00 , C11B1/10 , C13K1/02
Abstract: Processes for recovering sugars and nicotine from a tobacco biomass include feeding a biomass of tobacco plants and subcritical water to a reactor, hydrolyzing the biomass of tobacco plants with the subcritical water at a temperature between about 150° C. and 305° C. and recovering a liquid product and a solid product from the reactor, wherein the liquid product contains water-soluble sugars and nicotine.
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公开(公告)号：US20170253829A1
公开(公告)日：2017-09-07
申请号：US15595133
申请日：2017-05-15
Applicant: Tyton Biosciences, LLC
Inventor： Sandeep Kumar , Peter J. Majeranowski
CPC classification number: C11B1/104 , A23J1/14 , C10L5/44 , C10L5/447 , C10L2200/0484 , C10L2290/02 , C10L2290/544 , C10L2290/545 , C11B1/02 , C11B1/04 , C11B1/08 , C11B1/10 , C11B3/00 , C11C3/003 , Y02E50/10 , Y02E50/30
Abstract: Provided herein are methods of optimizing energy recovery from oilseeds. The methods disclosed provide at least the ability to swell oilseeds and disrupt the cell walls (hulls) without changing the functionality and quality of oil; the process integration of removing the impurities from oilseeds, oil extraction, protein hydrolysis, and green coal production to maximize the energy recovery in the form of crude oil, polypeptides, oligopeptides, amino acids, and/or green coal from oilseeds; and heat integration during processing stages including subcritical water pretreatment, oil extraction, subcritical water post-treatment, and subcritical water carbonization to minimize the process heat requirement.
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公开(公告)号：US10322395B2
公开(公告)日：2019-06-18
申请号：US15822414
申请日：2017-11-27
Applicant: Tyton Biosciences, LLC
Inventor： Sandeep Kumar , Peter Majeranowski , Igor Kostenyuk , Iulian Bobe , Florin Barla
IPC: B01J19/00 , C10G7/06 , C12P7/10 , C12P7/64 , C12M1/00 , D21D1/14 , D21H11/02 , D21C1/02 , D21C3/02 , D21C3/22 , D21C5/02 , D21C7/00 , D21C11/00 , B01D11/02 , C10G1/04 , D21B1/14 , C10G1/06
Abstract: The presently disclosed subject matter relates to an industrial system for processing various plant materials to produce marketable materials. Particularly, the system integrates subcritical water extraction technology and includes a pre-processing module and a two-stage extractor (processing module) with constant control of temperature, pressure, and/or residence time. In some embodiments, the final product of the disclosed system can include feedstock constituents for biofuel production (sugars and/or oil), biochar, raw materials for various industries (such as pulp for manufacturing paper or cellulose for use in various industries). The disclosed system can be modular or non-modular, stationary or mobile, and can include prefabricated elements with programmed automatic or manual operation so that it can be easily moved and/or assembled on site.
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公开(公告)号：US09518304B2
公开(公告)日：2016-12-13
申请号：US14723397
申请日：2015-05-27
Applicant: Tyton Biosciences, LLC
Inventor： Hsi-Chuan Chen , Peter Majeranowski , Igor Kostenyuk , Iulian Bobe
Abstract: Compositions and methods are provided for enhanced production of ethanol from fermentation of tobacco biomass. Nicotine resistant microorganisms are provided, as well as methods for making these nicotine resistant microorganisms. A biologically pure culture is provided of a nicotine resistant Saccharomyces cerevisiae strain or a mutant thereof having all the identifying characteristics thereof. Methods are provided for producing ethanol from fermentation of tobacco biomass in which the nicotine resistant microorganisms are used in the fermentation of the tobacco biomass, wherein a higher amount of ethanol can be produced from the fermentation. The nicotine resistant yeast strains of the present disclosure can improve ethanol production in tobacco biomass extract fermentations and shorten the fermentation time.
Abstract translation: 提供了组合物和方法，用于从烟草生物质发酵中增强生产乙醇。 提供了尼古丁抗性微生物，以及制备这些耐受尼古丁的微生物的方法。 提供具有所有鉴定特征的尼古丁耐药酿酒酵母菌株或其突变体的生物学纯培养物。 提供了用于从烟草生物质的发酵中生产乙醇的方法，其中在烟草生物质的发酵中使用尼古丁抗性微生物，其中可以从发酵产生更高量的乙醇。 本公开的尼古丁耐药酵母菌株可以改善烟草生物质提取物发酵中的乙醇生产并缩短发酵时间。
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10.发明授权公开(公告)号：US09328312B2
公开(公告)日：2016-05-03
申请号：US14437846
申请日：2013-10-15
Applicant: Tyton Biosciences, LLC
Inventor： Sandeep Kumar , Sergiy Popov , Peter J. Majeranowski , Igor Kostenyuk
CPC classification number: C11B1/104 , C10L5/44 , C10L2200/0484 , C10L2290/544 , C11B1/04 , C11B1/10 , Y02E50/10 , Y02E50/30
Abstract: Provided herein are methods of optimizing energy recovery from oilseeds. The methods disclosed provide at least the ability to swell oilseeds and disrupt the cell walls (hulls) without changing the functionality and quality of oil; the process integration of oil extraction and green coal production to maximize the energy recovery in the form of crude oil and green coal from oilseeds; and heat integration during processing stages including subcritical water pretreatment, oil extraction, and subcritical water carbonization to minimize the process heat requirement.
Abstract translation: 本文提供了优化来自油籽的能量回收的方法。 所公开的方法至少提供膨胀油籽并破坏细胞壁（船体）而不改变油的功能和质量的能力; 石油开采和绿色煤炭生产过程整合，最大限度地发挥油籽原油和绿色煤的能源回收; 和加工阶段的热集成，包括亚临界水预处理，抽油和亚临界水碳化，以最小化工艺热需求。
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